Abstract
A bacterial strain NF3 was isolated from a sludge sample from Inlet Admiralty Bay (Antarctica) and has been identified as belonging to the genus Alteromonas. The isolate was a Gram-negative rod with a single polar flagellum arrangement. The strain was catalase and oxidase positive and capable of gelatin hydrolysis. The G + C content was 42 mol%. The fatty acid composition and the protein profile has confirmed its identification as Alteromonas sp. During cell growth, the strain produced an exopolymer composed of 76–86% protein and 8–14% sugars depending on the medium used.
Similar content being viewed by others
References
Baumann L, Baumann P, Mandel M, Allen RD (1972) Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
Baumann P, Gauthier MJ, Baumann L (1984) Genus Alteromonas In: Murray RGE, Brenner DJ, Bryant MP, Holt JG, Krieg NR, Moulder JW, Pfennig N, Sneath PHA, Staley JT, Lapage SP, Lautrop H, Liston J, Niven CF (eds) Bergey's Manual of Systematic Bacteriology, vol 1. Williams and Wilkins, Baltimore, pp 343–352
Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4:330–334
Chan KY, Baumann L, Garza MM, Baumann P (1978) Two new species of Alteromonas: Alteromonas espejiana and Alteromonas undina. Int J Syst Bacteriol 28:217–222
Corpe WA (1970) An acid polysaccharide produced by primary film-forming marine bacterium. Dev Ind Microbiol 2:299–324
Cowan, Steel's (1974) Manual for the identification of medical bacteria, 2nd edn. Cambridge University Press
Dische Z (1947) A new specific color reaction of hexuronic acid. J Biol Chem 167:189–198
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Ferragut C, Leclerc H (1976) Etude comparative des méthodes de determination du Tm de l'ADN bactérien. Ann Microbiol 127:223–235
Gauthier MJ (1976) Alteromonas rubra sp.nov., a new marine antibiotic-producing bacterium. Int J Syst Bacteriol 26:459–466
Gauthier MJ, Breittmayer VA (1979) A new antibiotic-producing bacterium from seawater: Alteromonas aurantia sp.nov. Int J Syst Bacteriol 29:366–372
Gauthier MJ, Breittmayer VA (1991) The genera Alteromonas and Marinomonas. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The Prokaryotes, 2nd edn, vol 3. Springer-Verlag, Berlin, pp 3046–3084
Hugh R, Leifson E (1953) The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bact 66:24–26
Kóvacs N (1956) Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature (Lond) 178:703
Lelliot RA, Billing E, Hayward AC (1966) A determinative scheme for the fluorescent plant phatogenic Pseudomonads. J App Bacteriol 29:470–489
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin reagent. J Biol Chem 193: 265–275
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218
Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118
Mian FA, Jarman TR, Righelato RC (1978) Biosynthesis of Exopolysaccharide by Pseudomonas aeruginosa. J Bacteriol 134:418–422
Owen RJ, Hill LR (1979) The estimation of base composition, base pairing and genome size of bacterial deoxyribonucleic acids. In: Skinner FA, Lovelock DW (eds) Identification methods for microbiologists, 2nd edn. Academic Press, London
Owen RJ, Pitcher D (1985) Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. In: Goodfellow M, Minnikin E (eds) Chemical methods in bacterial systematic. Academic Press, London, pp 67–93
Pot B, Kersters K (1991) Practical exercise “SDS-PAGE Protein patterns of microorganisms”. Advanced course on chemotaxonomic and molecular methods in bacterial taxonomy. Coimbra, 16–27 Sept
Read R, Costerton JW (1987) Purification and characterization of adhesive exopolysaccharides from Pseudomonas putida and Pseudomonas fluorescens. Can J Microbiol 33:1080–1090
Sambrook J, Fritson EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press
Sierra G (1957) A simple method for the detection of lipolytic activity of microorganisms and some observations on the influence of contact between cells and fatty substrates. Antonie Van Leuwenhoek 23:37–52
Sutherland IW (1979) Microbial exopolysaccharide. In: Berkeley RCW, Gooday GW, Euwood DC (eds) Microbial polysaccharides and polysaccharides
Sutherland IW (1982) Biosynthesis of microbial exopolysaccharides. In: Rose, Garred (eds) Advances in microbial physiology, vol 23, Academic Press, London
Vernon FK, Bernlohr RW (1977) A new spectrophotometric assay for protein in cell extracts. Anal Biochem 82:362–371
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bozal, N., Manresa, A., Castellvi, J. et al. A new bacterial strain of Antarctica, Alteromonas sp. that produces a heteropolymer slime. Polar Biol 14, 561–567 (1994). https://doi.org/10.1007/BF00238226
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00238226