Abstract
Molecular analysis of the transgenes bar and gus was carried out over successive generations in six independent transgenic lines of wheat, until the plants attained homozygosity. Data on expression and integration of the transgenes is presented. Five of the lines were found to be stably transformed, duly transferring the transgenes to the next generation. The copy number of the transgenes varied from one to five in the different lines. One line was unstable, first losing expression of and then eliminating both the transgenes in R3 plants. Although the gus gene was detected in all the lines, GUS expression had been lost in R2 plants of all but one line. Rearrangement of transgene sequences was observed, but it had no effect on gene expression. All the stable lines were found to segregate for transgene activity in a Mendelian fashion.
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References
Becker D, Brettschneider R, Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant Jour 5:299–307
Castillo AM, Vasil V, Vasil IK (1994) Rapid production of fertile transgenic plants of rye (Secale cereale L.). Bio/Technol 12:1366–1371
Cedar H (1988) DNA methylation and gene activity. Cell 53:3–4
Christensen AH, Sharrock RA, Quail PH (1992) Maize ubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol 18:675–689
Cooley J, Ford T, Christou P (1995) Molecular and genetic characterization of elite transgenic rice plants produced by electric discharge particle acceleration. Theor Appl Genet 90:97–104
Finnegan J, McElroy D (1994) Transgene inactivation: plants fight back! Bio/Technol 12:883–888
Flavell RB (1994) Inactivation of gene expression in plants as a consequence of specific gene duplication. Proc Natl Acad Sci USA 91:3490–3496
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Inheritance and expression of chimeric genes in the progenies of transgenic maize plants. Bio/Technol 8:833–839
Goto F, Toki S, Uchimiya H (1993) Inheritance of a co-transferred foreign gene in the progenies of transgenic rice plants. Transgenic Res 2:300–305
Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
Lassner NW, Peterson P, Yoder JI (1989) Simultaneous amplification of multiple DNA fragments by polymerase chain reaction in the analysis of transgenic plants and their progeny. Plant Mol Biol Rep 7:116–128
Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant Jour 5:285–297
Register III JC, Peterson DJ, Bell PJ, Bullock WP, Evans BF, Greenland AJ, Higgs NS, Jepson I, Jiao S, Lewnau CJ, Sillick JM, Wilson HM (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25:951–961
Spencer TM, O'Brien JV, Start WG, Adams TR, Gordan-Kamm WJ, Lemaux PG (1992) Segregation of transgenes in maize. Plant Mol Biol 18:201–210
Vasil IK (1994) Molecular improvement of cereals. Plant Mol Biol 25:925–937
Vasil V, Castillo AM, Fromm ME, Vasil I K (1992) Herbicideresistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Biol/Technol 10:667–674
Vasil V, Srivastava V, Castillo AM, Fromm ME, Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technol 11:1553–1558
Weeks JT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077–1084
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Communicated by G. Wenzel
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Srivastava, V., Vasil, V. & Vasil, I.K. Molecular characterization of the fate of transgenes in transformed wheat (Triticum aestivum L.). Theoret. Appl. Genetics 92, 1031–1037 (1996). https://doi.org/10.1007/BF00224045
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DOI: https://doi.org/10.1007/BF00224045