Summary
Histological, histochemical, and electron microscopical investigations of the rat pancreas, one to five hours after pilocarpine injection (4 mg in 1 ml normal saline solution), showed different secretory responses in periinsular and apoinsular acinar cells. The responsiveness to parasympathetic stimulation is markedly retarded and weakened in the periinsular area. It is supposed that the halo phenomenon is related not only to an inhibition of the secretion, but also to a longer lasting synthesis of zymogen granules. The insular hormones, glucagon and insulin, which are possibly locally enriched by insulo-acinar capillary anastomoses or by diffusion, may be involved in the regulation of activity in the periinsular cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alm, P., Cegrell, L., Ehinger, B., Falck, B.: Remarkable adrenergic nerves in the exocrine pancreas. Z. Zellforsch. 83, 178–186 (1967)
Bargmann, W.: Histologie und mikroskopische Anatomie des Menschen, 6. Aufl. Stuttgart: Thieme 1967
Becker, V.: Sekretionsstudien am Pankreas. Stuttgart: Thieme 1957
Burkl, W.: Über Zymogenhöfe in der menschlichen Bauchspeicheldrüse. Wien. klin. Wschr. 61, 264–267 (1949)
McCuskey, R. S., Chapman, T. M.: Microscopy of the living pancreas in situ. Amer. J. Anat. 126, 395–407 (1969)
Ferner, H.: Die orthologische Histologie und Cytologie der Langerhansschen Inseln. Verh. dtsch. Ges. Path. 42. Tagg. 1958, 25–41 (1959)
Goldfischer, S., Essner, E., Novikoff, A. B.: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem. 12, 72–95 (1964)
Hebb, C. O.: The effect of insulin administration on the response of the pancreas to parasympathic stimulation. Quart. J. exp. Physiol. 26, 339–354 (1937)
Hellman, B., Wallgren, A., Petersson, B.: Cytological characteristics of the exocrine pancreatic cells with regard to their position in relation to the islets of Langerhans. Acta endocr. (Kbh.) 39, 465–473 (1962)
Jarotsky, A. J.: Über die Veränderungen in der Größe und im Bau der Pankreaszellen mit einigen Arten der Inanition. Virchows Arch. path. Anat. 156, 407–450 (1899)
Kramer, M. F., Poort, C.: Protein synthesis in the pancreas of the rat after stimulation of secretion. Z. Zellforsch. 86, 475–486 (1968)
Kramer, M. F., Tan, H. T.: The peri-insular acini of the pancreas of the rat. Z. Zellforsch. 86, 163–170 (1968)
Legg, P. G.: Fluorescence studies on nerval structures and endocrine cells in the pancreas of the cat. Z. Zellforsch. 88, 487–495 (1968)
Nakajima, S., Magee, D. F.: Inhibition of exocrine pancreatic secretion by glucagon and D-glucose given intravenously. Canad. J. Physiol. 48, 299–305 (1970)
Nevalainen, T. J.: Effects of pilocarpine stimulation on rat pancreatic acinar cells. An electron microscopic study with morphometric analysis. Acta path, microbiol. scand., Sect. A, Suppl. 210 (1970)
Pearse, A. G. E.: Histochemistry, theoretical and applied. London: Churchill Ltd. 1961
Richter, K., Putzke, H.-P., Bienengräber, A.: Histochemische und elektronenmikroskopische Untersuchungen der Azinuszelle des Rattenpankreas im Hungerzustand. Exp. Path. 5, 192–199 (1971)
Said, F. M.: Tierexperimentelle Untersuchungen über den Einfluß des Inselorgans auf die exokrine Pankreasfunktion unter besonderer Berücksichtigung der juxtainsulären Azini am Modell der Inselschädigung durch Alloxan. Dissertation, Rostock 1974
Sergeyeva, M. A.: Microscopic changes in the pancreatic gland of the cat produced by sympathetic and parasympathetic stimulation. Anat. Rec. 71, 319–335 (1938)
Sobel, H.-J., Avrin, E.: Localization of acid phosphatase activity in rat pancreatic acinar cells: a light and electron microscopic study. J. Histochem. Cytochem. 13, 301–303 (1965)
Stahl, M.: Elektronenmikroskopische Untersuchungen über die vegetative Innervation der Bauchspeicheldrüse. Z. mikr.-anat. Forsch. 70, 62–102 (1963)
Stremmel, W.: Glukagon zur Behandlung der Pankreatitis. Münch. med. Wschr. 116, 69–70 (1974)
Thiel, A.: Untersuchungen über das Gefäß-System der Pankreasläppchens bei verschiedenen Säugern mit besonderer Berücksichtigung der Kapillarknäuel der Langerhansschen Inseln. Z. Zellforsch. 39, 339–372 (1954)
Watari, N., Baba, N.: Several findings on the fine structural alterations of the exocrine pancreas after the administration of some chemicals. J. Electron Micr. (Tokyo) 17, 327–341 (1968)
Wool, J. G., Stirewalt, W. S., Kurikara, K. L. R. B., Bailey, P., Oyer, D.: Mode of action of insulin in the regulation of protein biosynthesis in muscle. Recent Progr. Hormone Res. 24 139–208 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Putzke, HP., Said, F. Different secretory responses of periinsular and other acini in the rat pancreas after pilocarpine injection. Cell Tissue Res. 161, 133–143 (1975). https://doi.org/10.1007/BF00222119
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00222119