Abstract
The presence of polygalacturonase and its correlation with the formation of lateral roots in leek (Allium porrum L.) seedlings have been investigated. During root growth, a steady increase in polygalacturonase activity was associated with that of the lateral root primordia. Fractionation of root extract by fast protein liquid chromatography resolved at least two polygalacturonase isoforms. One of the isoforms, a 75-kdalton protein, strongly reacted on Western blots probed with a polyclonal antibody raised against tomato polygalacturonase. It also reacted with both polyclonal and monoclonal antisera raised against Fusarium moniliforme polygalacturonase. In situ localization with these three antibodies showed that polygalacturonase was present over the meristems of lateral root primordia. Antibodies against pectins (Knox et al. 1990, Planta 181, 512–521) detected large amounts of pectic material filling the area between the apex of the primordium and the mother root tissues. We suggest that a polygalacturonase plays an important role in leek root morphogenesis, particularly during lateral root outgrowth.
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Abbreviations
- FPLC:
-
fast protein liquid chromatography
- RGU:
-
one unit of polygalacturonase activity
- SDS-PAGE:
-
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
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The Authors are grateful to Dr. Dean Della Penna (Department of Vegetable Crops, University of California, Davis, Calif., USA) for generously providing the polyclonal antibody raised against the tomato polygalacturonase. This research was supported by National Research of Italy, Special project RAISA, Subproject N∘2, N∘360.
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Peretto, R., Favaron, F., Bettini, V. et al. Expression and localization of polygalacturonase during the outgrowth of lateral roots in Allium porrum L.. Planta 188, 164–172 (1992). https://doi.org/10.1007/BF00216810
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DOI: https://doi.org/10.1007/BF00216810