Summary
Using an antiserum directed against the C-terminus of hGRH(1–44)NH2 and another recognizing the mid portion to C-terminal of hGRH(1–40)OH, we identify two immunocytochemically distinct GRH-immunoreactive systems in the brain of the codfish, Gadus morhua. The antiserum directed against GRF(1–44)NH2 stains cell bodies exclusively in the rostral pars distalis. The other antiserum immunoreactive with GRF(1–40)OH reacts with a population of parvocellular and magnocellular neuronal cell bodies in the hypothalamus and with two major axonal pathways which project toward the median eminence and terminate primarily in the pars nervosa. These results indicate the presence of at least two forms of hGRH-like peptides in the teleost which may have different roles in the regulation of pituitary function.
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Abraham M, Dinari-Lavie V, Lotan R (1977) The pituitary of Aphanius dispar (Rüppell) from hypersaline marshes and freshwater. Cell Tissue Res 179:317–330
Ball JN (1981) Hypothalamic control of the pars distalis in fishes, amphibians, and reptiles. Gen Comp Endocrinol 44:135–170
Barnet FH, Sohn J, Reichlin S, Jackson IMD (1982) Three luteinizing hormone-releasing hormone like substances in a teleost fish brain: none identical with the mammalian LH-RH decapeptide. Biochem Biophys Res Commun 105:209–216
Batten T, Ball T (1975) Ultrastructure of the adenohypophysis in the teleost Poecilia latipinna. Cell Tissue Res 101:239–261
Böhlen P, Brazeau P, Bloch P, Ling N, Gaillard R, Guillemin R Biochem. (1983) Human hypothalamic growth hormone releasing factor (GRF): Evidence for two forms identical to tumor-derived GRF-44-NH2 and GRF-40. Biophys Res Commun 114:930–936
Cook AF, Peter RE (1983) Effects of hypothalamic lesions on serum growth hormone levels and growth rates in goldfish, Carassius auratus. Gen Comp Endocrinol 51:175–182
Crim JW, Urano A, Gorbman A (1979) Immunocytochemical studies of luteinizing hormone-releasing hormone in brains of Agnathan fishes. Gen Comp Endocrinol 38:290–299
Dubois MP, Billard R, Breton B, Peter RE (1979) Comparative distribution of somatostatin, LH-RH, neurophysin, and α-endorphin in the rainbow trout: an immunocytological study. Gen Comp Endocrinol 37:220–232
Fryer JW, Maler L (1981) Hypophysiotropic neurons in the goldfish hypothalamus demonstrated by retrograde transport of horseradish peroxidase. Cell Tissue Res 218:93–102
Gill VE, Burford GD, Lederis K, Zimmerman EA (1977) An immunocytochemical investigation for arginine vasotocin and neurophysin in the pituitary gland and the caudal neurosecretory system of Catostomus commersoni. Gen Comp Endocrinol 32:505–511
Goldman JA, Moltich ME, Thorner MO, Rivier J, Vale W, Reichlin S (1984) Growth hormone releasing hormone 1-40-OH(GHRH) is a prolactin (PRL) — releasing factor. Abstracts of 7th International Congress of Endocrinology p 606
Gossens N, Dierickx K, Vandesande F (1977) Immunocytochemical localization of vasotocin and isotocin in the preopticohypophysial neurosecretory system of teleosts. Gen Comp Endocrinol 32:371–375
Gubler U, Monahan JJ, Lomedico PT, Bhatt RS, Collier KJ, Hoffman BJ, Böhlen P, Esch F, Ling N, Zeytin F, Brazeau P, Poonian MS, Gage LP (1983) Cloning and sequence analysis of cDNA for the precursor of human growth hormone-releasing factors, somatocrinin. Proc Natl Acad Sci 80:4311–44314
Guillemin R, Brazeau P, Böhlen P, Esch F, Ling N, Wehrenberg WB (1982) Growth hormone releasing factor from a human pancreatic tumor that caused acromegaly. Science 218:585–587
Guillemin R, Ling N, Esch F, Böhlen P, Brazeau P, Wehrenberg W, Baird A, Bloch B, Mougin C, Zeytin F (1984a) Growth hormone-releasing factor. An update. Abstracts of 7th International Congress of Endocrinology p 179
Guillemin R, Zeytin F, Ling N, Böhlen P, Esch F, Brazeau P, Bloch B, Wehrenberg WB (1984b) Growth hormone-releasing factor: chemistry and physiology. Proc Soc Exp Biol Med 175:407–413
Harrison RJ, Young BA (1969) Stellate cells in the Delphinid adenohypophysis. J Endocrinol 43:323–324
King JA, Millar RP (1980) Comparative aspects of luteinizing hormone-releasing hormone structure and function in vertebrate phylogeny. Endocrinology 106:707–717
King JC, Lechan RM, Kugel G, Anthony ELP (1983) Acrolein: a fixative for immunocytochemical localization of peptides in the central nervous system. J Histochem Cytochem 31:62–68
Leatherland JF, Percy R (1976) Structure of the nongranulated cells in the hypophyseal rostral pars distalis of cyclostomes and actinopterygians. Cell Tissue Res 166:185–200
Lin HD, Bollinger J, Ling N, Reichlin S (1984) Immunoreactive growth hormone-releasing factor in human stalk median eminence. J Clin Endocrinol Metab 58:1197–1199
Mayo KE, Vale W, Rivier J, Rosenfeld MG, Evans RM (1983) Expression cloning and sequence of a cDNA encoding human growth hormone-releasing factor. Nature 306:86–88
Pan JX, Lechan R, Lin HD, Reichlin S, Jackson IMD (1984) Human pancreatic growth hormone releasing factor (hpGRH) in teleost brain and pituitary: multiple molecular forms and two distinct neuronal systems. Abstracts of 7th International Congress of Endocrinology, p 1154
Rivier J, Spiess J, Thorner M, Vale W (1982) Characterization of a growth hormone releasing factor from a human pancreatic islet tumor. Nature (Lond.) 300:276–278
Sherwood N, Eiden L, Brownstein M, Spiess J, Rivier J, Vale W (1983) Characterization of a teleost gonadotropin-releasing hormone. Proc Natl Acad Sci (USA) 80:2794–2798
Singh IJ, Haider LS (1977) Hypothalamo-hypophysial neurosecretory system of the teleost Trichagaster fasciatus. Z Mikrosk Anat Forsch 91:509–520
Spiess J, Rivier J, Vale W (1983) Characterization of rat hypothalamic growth hormone-releasing factor. Nature (Lond.) 303:532–535
Stachura ME, Tyler JM, Farmer PK, Kent PG, Lee YH (1983) Somatocrinin preferentially stimulates secretion of newly synthesized growth hormone and increases prolactin release in vitro. Clin Res 31:870A
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyers HG (1970) The unlabeled antibody-enzyme method of immunocytochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in the identification of spirochetes. J Histochem Cytochem 18:315–333
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Pan, J.X., Lechan, R.M., Lin, H.D. et al. Immunoreactive neuronal pathways of growth hormone-releasing hormone (GRH) in the brain and pituitary of the teleost Gadus morhua . Cell Tissue Res. 241, 487–493 (1985). https://doi.org/10.1007/BF00214567
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DOI: https://doi.org/10.1007/BF00214567