Abstract
To examine the degree of conservation of gene organization in and around the class II regions of the major histocompatibility complexes of mouse and human, we have established the positions of sequences homologous to five human non-class II genes (RING1-5) in mouse, and the positions of sequences homologous to three mouse non-class II genes (KE3-5) in human. The resulting comparative map reveals that the organization of genes in the entire proximal region of the MHCs of mouse and human is remarkably conserved, apart from the H-2K gene pair in mouse, which can be accounted for by a 60 kilobase (kb) insertion. The characterization of the novel human gene RING5 is also presented. This gene, which is widely expressed, maps 85 kb proximal to the DPB2 gene. Partial nucleotide sequencing of a RING5 cDNA clone reveals that it is the human homolog of the mouse KE4 gene.
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Abe, K., Wei, J.-F., Wei, F.-S., Hsu, Y.-C., Uehara, H., Artzt, K., and Bennett, D.: Searching for coding sequences in the mammalian genome: the H-2K region of the mouse MHC is replete with genes expressed in embryos. EMBO J 7: 3441–3449, 1988
Artzt, K., Abe, K., Uehara, H., and Bennett, D.: Intra-H-2 recombination in t haplotypes shows a hot spot and close linkage of l tw5 to H-2K. Immunogenetics 28: 30–37, 1988
Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Smith, J. A., Seidman, J. G., and Struhl, K. (eds.): Current Protocols in Molecular Biology, Wiley Interscience, New York, 1987
Bodmer, W. F.: HLA structure and function: a contemporary view. Tissue Antigens 17: 9–20, 1981
Bodmer, W. F., Trowsdale, J., Young, J., and Bodmer, J.: Gene cluster and the evolution of the major histocompatibility system. Phil Trans R Soc Lond Biol 312: 313–315, 1986
Denaro, M., Gustafsson, K., Larhammar, D., Steinmetz, M., Peterson, P. A., and Rask, L.: Mouse MHC class II gene Eβ2 is closely related to Eβ and to HLA-DR. Immunogenetics 21: 613–616, 1985
Deverson, T., Gow, I., Coadwell, J., Monaco, J. J., Butcher, G. W., and Howard, J. C.: The class II region of the MHC encodes proteins related to the multidrug resistance series of transmembrane transporters. Nature 348: 738–741, 1990
Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983
Hanson, I. M., Gorman, P., Lui, V. C. H., Cheah, K. S. E., Solomon, E., and Trowsdale, J.: The human α2(XI) collagen gene (COL11A2) maps to the centromeric border of the major histocompatibility complex on chromosome 6. Genomics 5: 925–931, 1989
Hanson, I. M., Poustka, A., and Trowsdale, J.: New genes in the class II region of the human major histocompatibility complex. Genomics, in press, 1991
Hardy, D. A., Bell, J. I., Long, E. O., Lindsten, T., and McDevitt, H. O.: Mapping of the class II region of the human major histocompatibility complex by pulse-field gel electrophoresis. Nature 323: 453–455, 1986
Kaufman, J. F., Auffray, C., Korman, A. J., Shackleford, D. A., and Strominger, J.: The class II molecules of the human and murine major histocompatibility complex. Cell 36: 1–13, 1984
Klein, J.: Natural History of the Major Histocompatibility Complex, Wiley, New York, 1986
Koller, B. H., Geraghty, D., Orr, H. T., Shimuzu, Y., and DeMars, R.: Organization of the human class I major histocompatibility complex genes. Immunol Res 6: 1–10, 1987
Larhammar, D., Hammerling, U., Rask, L., and Perterson, P. A.: Sequence of gene and cDNA encoding murine major histocompatibility complex class II gene Aβ2. J Biol Chem 260: 14111–14119, 1986
St.-Jacques, B., Han, T.-H., MacMurray, A., and Shin, H.-S.: A putative transmembrane protein with histidine rich charge clusters encoded in the H-2K/t w5region of mice. Mol Cell Biol 10: 138–145, 1990
Sambrook, J., Fritsch, E. F., Maniatis, T.: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1989
Seed, B.: An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2. Nature 329: 840–842, 1987
Steinmetz, M., Stephan, D., and Fischer-Lindahl, K.: Gene organization and recombinational hot spots in the murine major histocompatibility complex. Cell 44: 895–904, 1986
Tonnelle, C., DeMars, R., and Long, E.: DOβ: a new β chain gene in HLA-D with a distinct regulation of expression. EMBO J 4: 2839–2847, 1985
Trowsdale, J. and Kelly, A.: The human HLA class II α chain gene DZα is distinct from genes in the DP, DQ, and DR subregions. EMBO J 4: 2231–2237, 1985
Trowsdale, J., Hanson, I., Mockridge, I., Beck, S., Townsend, A., and Kelly, A.: Sequences encoded in the class II region of the MHC related to the ‘ABC’ family of transporters. Nature 348: 741–744, 1990
Weiss, E. H., Golden, L., Fahrner, K., Mellor, A. L., Devlin, J. J., Bullman, H., Tiddens, H., Bud, H., and Flavell, R. A.: Organization and evolution of the class I genes family in the major histocompatibility complex of the C57BL/10 mouse. Nature 310: 650–655, 1984
Widera, G. and Flavell, R. A.: The I region of the C57BL/10 mouse: characterization and physical linkage to H-2K of an SBβ-like class II pseudogene, =Aβ3. Proc Natl Acad Sci USA 82: 5500–5504, 1985
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M58660.
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Hanson, I.M., Trowsdale, J. Colinearity of novel genes in the class II regions of the MHC in mouse and human. Immunogenetics 34, 5–11 (1991). https://doi.org/10.1007/BF00212306
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DOI: https://doi.org/10.1007/BF00212306