Abstract
In two pot-culture experiments with maize in a silty loam (P2 soil) contaminated by atmospheric deposition from a metal smelter, root colonization with indigenous or introduced arbuscular mycorrhizal (AM) fungi and their influence on plant metal uptake (Cd, Zn, Cu, Pb, Mn) were investigated. Soil was γ-irradiated for the nonmycorrhizal control. In experiment 1, nonirradiated soil provided the mycorrhizal treatment, whereas in experiment 2 the irradiated soil was inoculated with spores of a fungal culture from P2 soil or a laboratory reference culture, Glomus mosseae. Light intensity was considerably higher in experiment 2 and resulted in a fourfold higher shoot and tenfold higher root biomass. Under the conditions of experiment 1, biomass was significantly higher and Cd, Cu, Zn and Mn concentrations significantly lower in the mycorrhizal plants than in the nonmycorrhizal plants, suggesting a protection against metal toxicity. In contrast, in experiment 2, biomass did not differ between treatments and only Cu root concentration was decreased with G. mosseae-inoculated plants, whereas Cu shoot concentration was significantly increased with the indigenous P2 fungal culture. The latter achieved a significantly higher root colonization than G. mosseae (31.7 and 19.1%, respectively) suggesting its higher metal tolerance. Zn shoot concentration was higher in both mycorrhizal treatments and Pb concentrations, particularly in the roots, also tended to increase with mycorrhizal colonization. Cd concentrations were not altered between treatments. Cu and Zn, but not Pb and Cd root-shoot translocation increased with mycorrhizal colonization. The results show that the influence of AM on plant metal uptake depends on plant growth conditions, on the fungal partner and on the metal, and cannot be generalized. It is suggested that metal-tolerant mycorrhizal inoculants might be considered for soil reclamation, since under adverse conditions AM may be more important for plant metal resistance. Under the optimized conditions of normal agricultural practice, however, AM colonization even may increase plant metal absorption from polluted soils.
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References
Alexander M (1982) Most probable number method for microbial populations. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, vol. 2: Chemical and microbiological properties. American Society of Agronomy, Madison, Wis, pp 815–820
Bethlenfalvay GJ, Franson RL (1989) Manganese toxicity alleviated by mycorrhizae in soybean. J Plant Nutr 12:953–970
Bowen HJM, Cawse PA (1964) Some effects of gamma radiation on the composition of the soil solution and soil organic matter. Soil Sci 98:358–361
Bradley R, Burt AJ, Read DJ (1982) The biology of mycorrhiza in the Ericaceae. VIII. The role of mycorrhizal infection in heavy metal resistance. New Phytol 91:197–209
Commission of the European Community (CEC) (1986) Council directive on the protection of the environment and in particular of the soil, when sewage sludge is used in agriculture. Official J Eur Communities L181:6–12
Cooper KM, Tinker PB (1978) Translocation and transfer of nutrients in vesicular-arbuscular mycorrhizas. II. Uptake and translocation of phosphorus, zinc and sulphur. New Phytol 81:43–52
Dueck TA, Visser P, Ernst WHO, Schat H (1986) Vesicular-arbuscular mycorrhizae decrease zinc-toxicity to grasses growing in zinc-polluted soil. Soil Biol Biochem 18:331–337
El-Kherbawy M, Angle JS, Heggo A, Chaney RL (1989) Soil pH, rhizobia, and vesicular-arbuscular mycorrhizae inoculation effects on growth and heavy metal uptake of alfalfa (Medicago sativa L.). Biol Fertil Soils 8:61–65
Faber BA, Zasoski RJ, Burau RG, Uriu K (1990) Zinc uptake by corn affected by vesicular-arbuscular mycorrhizae. Plant Soil 129:121–130
Gildon A, Tinker PB (1983) Interactions of vesicular-arbuscular mycorrhizal infection and heavy metals in plants. I. The effect of heavy metals on the development of vesicular arbuscular mycorrhizas. New Phytol 95:247–261
Graham JH, Timmer LW, Fardelman D (1986) Toxicity of fungicidal copper in soil to citrus seedlings and vesicular-arbuscular mycorrhizal fungi. Phytopathology 76:66–70
Harley JL, Harley EL (1987) A check-list of mycorrhiza in the British flora. New Phytol [Suppl] 105:1–102
Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Calif Agric Exp Sat Circ 347:1–32
Killham K (1985) Vesicular-arbuscular mycorrhizal mediation of trace and minor element uptake in perennial grasses: relation to livestock herbage. In: Fitter AH, Atkinson D, Read DJ, Usher MB (eds) Ecological interactions in soil: plants, microbes and animals. Blackwell, Palo Alto, Calif, pp 225–232
Killham K, Firestone MK (1983) Vesicular-arbuscular mycorrhizal mediation of grass response to acidic and heavy metal deposition. Plant Soil 72:39–48
Kothari SK, Marschner H, Römheld V (1990) Direct and indirect effects of VA mycorrhizal fungi and rhizosphere microorganisms on acquisition of mineral nutrients by maize (Zea mays L.) in a calcareous soil. New Phytol 116:637–646
Kothari SK, Marschner H, Römheld V (1991) Contribution of the VA mycorrhizal hyphae in acquisition of phosphorus and zinc by maize grown in a calcerous soil. Plant Soil 131:177–185
Kraffczyk I, Trolldenier G, Beringer H (1984) Soluble root exudates of maize: Influence of potassium supply and rhizosphere microorganisms. Soil Biol Biochem 16:315–322
Laheurte F, Berthelin J (1988) Effect of a phosphate solubilizing bacteria on maize growth and root exudation over four levels of labile phosphorus. Plant Soil 105:11–17
Leyval C, Berthelin J (1982) Role of symbiotic and nonsymbiotic microflora on biotite weathering and maize growth: influence of plant growth conditions. Sci Sol 1:95–96
Leyval C, Berthelin J, Schontz D, Weissenhorn I, Morel JL (1991) Influence of endomycorrhizas on maize uptake of Pb, Cu, Zn and Cd applied as mineral salts or sewage sludges. In: Farmer JG (ed) Heavy metals in the environment. CEP Consultants, Edinburgh, pp 204–207
Li X-L, Marschner H, Römheld V (1991) Acquisition of phosphorus and copper by VA-mycorrhizal hyphae and root-to-shoot transport in white clover. Plant Soil 136:49–57
Marschner H (1986) Mineral nutrition of higher plants. Academic Press, London
McGee PA (1987) Alteration of growth of Solanum opacum and Plantago drummondii and inhibition of regrowth of hyphae of vesicular-arbuscular mycorrhizal fungi from dried root pieces by manganese. Plant Soil 101:227–233
Mench M, Morel JL, Guckert A (1987) Metal binding properties of high molecular weight soluble exudates from maize (Zeamays L.) roots. Biol Fertil Soils 3:165–169
Morel JL, Mench M, Guckert A (1986) Measurements of Pb2+, Cu2+ and Cd2+ binding with mucilage exudates from maize (Zea mays L.) roots. Biol Fertil Soils 2:29–34
Olsen SR, Cole CV, Watenabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Dept Agric Circ 939
Perrin R (1990) Interactions between mycorrhizae and diseases caused by soil-born fungi. Soil Use Manag 6:189–195
Rauser WE, Glover J (1984) Cadmium binding protein in roots of maize. Can J Bot 62:1645–1650
Rogers RD, Williams SE (1986) Vesicular-arbuscular mycorrhiza: influence on plant uptake of caesium and cobalt. Soil Biol Biochem 18:371–376
Rovira AD, Davey CB (1974) Biology of the rhizosphere. In: Carson EW (ed) The plant root and its environment. University of Virginia, Charlottesville, Va, p 153–204
Sanders FE, Tinker PB, Black RLB, Palmerley (1977) The development of endomycorrhizal root systems. I. Spread of infection and growth-promoting effects with four species of vesicular-arbuscular endophytes. New Phytol 78:257–268
Sauerbeck D (1982) Welche Schwermetallgehalte in Pflanzen dürfen nicht überschritten werden, um Wachstumsbeeinträchtigungen zu vermeiden? Landwirtschaftl Forsch 39:105–129
Schönbeck F, Dehne HW (1981) Mycorrhiza and plant health. Gesunde Pflanz 33:186–190
Schüepp H, Dehn B, Sticher H (1987) Interaktionen zwischen VA-Mykorrhizen und Schwermetallbelastungen. Angew Bot 61:85–95
Tinker PB, Gildon A (1983) Mycorrhizal fungi and ion uptake. In: Robb DA, Pierpoint WS (eds) Metals and micronutrients. Uptake and utilization of metals by plants. Academic Press, London, pp 21–32
Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986) Mesure du taux de mycorhization VA d'un système radiculaire. Recherche de méthodes d'estimation ayant une signification fonctionelle. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Physiological and genetical aspects of mycorrhizae. INRA, Paris, pp 217–221
Turnau K, Kottke I, Oberwinkler F (1993) Element localization in mycorrhizal roots of Pteridium aquilinum (L.) Kuhn collected from experimental plots treated with cadmium dust. New Phytol 123:313–324
Weissenhorn I, Leyval C, Berthelin J (1993) Cd-tolerant arbuscular mycorrhizal (AM) fungi from heavy-metal polluted soils. Plant Soil 157:247–256
Weissenhorn I, Leyval C, Berthelin J (1995) Bioavailability of heavy metals and abundance of arbuscular mycorrhiza (AM) in a soil polluted by atmospheric deposition from a smelter. Biol Fertil Soils 19:22–28
Wilkinson L (1989) SYSTAT: The system for statistics. SYSTAT, Evanston, Ill
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Weissenhorn, I., Leyval, C., Belgy, G. et al. Arbuscular mycorrhizal contribution to heavy metal uptake by maize (Zea mays L.) in pot culture with contaminated soil. Mycorrhiza 5, 245–251 (1995). https://doi.org/10.1007/BF00204957
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DOI: https://doi.org/10.1007/BF00204957