Abstract
Calli were formed on the explants of midrib, meristem and immature stipe parts from freshly collected Undaria pinnatifida sporophytes. Each part was sterilized by Betadine and ethanol, and was cut into explants. The explants were incubated on an agar medium at 10 hours light and 14 hours dark photoperiod under a photon flux density of 80 µmol m−2 s−1. Callus was formed best on the explants of meristem parts at a temperature of 13 °C on PESI medium. Calli were cut off from the explants and were transferred into a sterile liquid PESI medium in flasks. Callus was dark brown in colour and was composed of well-pigmented cells. The cells were loosely bound and were separated by low power sonication, and were easy to attach to vinylon strings. From the calli formed on the explants of meristem parts, entire fronds were regenerated, but from the calli formed on the explants of midrib parts, only thin layered laminae were regenerated. The calli formed on the explants of immature stipe parts did not exhibit any regeneration at all.
Similar content being viewed by others
References
Fang Z., Z. Yan & Z. Wang, 1983. Some preliminary observations on tissue culture in Laminaria japonica and Undaria pinnatifida. Kexue Tongbao 28: 247–249.
Fries, L., 1980. Axenic tissue cultures from the sporophytes of Laminaria digitata and Laminaria hyperborea (Phaeophyta). J. Phycol. 16: 475–477.
Ishikawa, Y. & N. Saga, 1989. The diseases of economically valuable Seaweeds and pathology in Japan. In S. Miyachi, I. Karube & Y. Ishida (eds), Current Topics in Marine Biotechnology. The Japanese Society for Marine Biotechnology, Tokyo: 215–218.
Iwasaki, H., 1961. The life cycle of Porphyra tenera in vitro. Biol. Bull. 121: 173–187.
Kain, J. M., 1991. Cultivation of attached Seaweeds. In M. J. Guiry & G. Blunden (eds), Seaweed resources in Europe: Uses and potential. J. Wiley & Sons, Chichester: 309–377.
Kawashima, Y. & H. Tokuda, 1990. Callus formation in Ecklonia cava Kjellman (Laminariales, Phaeophyta). Hydrobiologia 204/205: 375–380.
Kawashima, Y. & H. Tokuda, 1992. Effects of plant hormones on the induction of adventitious embryos from calluses of a brown algae, Ecklonia cava (Laminariales). Oceanis 18: 1–9.
Lawlor, H. J., J. A. McComb & M. A. Borowitzka, 1988. The development of filamentous and callus-like growth in Axenic tissue culture of Ecklonia radiata (Phaeophyta). In T. Stadler, J. Mollion, M.-C. Verdus, Y. Karamanos, H. Morvan & D. Christiaen (eds), Algal Biotechnology. Elsevier Applied Science, London & New York: 139–150.
Lawlor, H. J., J. A. McComb & M. A. Borowitzka, 1989. Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins. J. appl. Phycol. 1: 105–112.
Lee, T. F., 1985. Aposporous gametophyte formation in stipe explants from Laminaria saccharina (Phaeophyta). Bot. Mar. 28: 179–185.
McLean, R. O. & D. J. Connolly, 1989. Callus and suspension culture in the Laminariales. XIIIth Int. Seaweeds Symp., Programme & Abstracts: A-98.
Notoya, M., 1988. Tissue culture from the explants of Ecklonia stolonifera Okamura (Phaeophyta, Laminariales). Jap. J. Phycol. 36: 175–177.
Notoya, M. & Y. Aruga, 1989. Tissue culture from the explants of Ecklonia cava Kjellman (Laminariales, Phaeophyta). Jap. J. Phycol. 37: 302–304.
Notoya, M. & Y. Aruga, 1990. Tissue culture from the explant of stipe of Eisenia bicyclis (Kjellman) Setchell (Laminariales, Phaeophyta). Jap. J. Phycol. 38: 387–390.
Ohkubo, T., 1992. Market of Wakame in Japan. Nori & Sea vegetables. 39: 1–8. (in Japanese).
Polne-Fuller, M. & A. Gibor, 1986. Tissue culture of seaweeds. In K. Bird & P. H. Benson (eds), Seaweed cultivation for renewable resources. Gas Research Institute, Chicago.: 219–239.
Polne-Fuller, M. & A.Gibor, 1987. Callus and callus-like growth in Seaweeds: induction and culture. Hydrobiologia 151/152: 131–138.
Polne-Fuller, M., N. Saga & A. Gibor, 1986. Algal cell and tissue cultures and selection of algal strains. Bein. Nova Hedwigia 83: 30–36.
Provasoli, L., 1963. Growing marine Seaweeds. Proc. int. Seaweed Symp. 4: 9–14.
Saga, N. & Y. Sakai, 1983. Axenic tissue culture and callus formation of the marine brown alga Laminaria angustata. Bull. Jan. Soc. Sci. Fish. 49: 1561–1563.
Saga, N., T. Uchida & Y. Sakai, 1978. Clone Laminaria from single isolated cell. Bull. Jap. Soc. Sci. Fish. 44: 87.
Tatewaki, M., 1966. Formation of a crustaceous sporophyte with unilocular sporangia in Scytosiphon lomentaria. Phycologia 6: 62–66.
Tokuda, H. & Y. Kawashima, 1988. Protoplast isolation and culture of a brown alga, Undaria pinnatifida. In T.Stadler, J. Mollion, M.-C. Verdus, Y. Karamanos, H. Morvan & D. Christiaen (eds), Algal Biotechnology. Elsevier Applied Science, London & New York: 151–157.
Tokuda, H., M. Ohno & H. Ogawa, 1987. The Resources and Cultivation of Seaweeds. Midori Shobo, Tokyo.: 136. (in Japanese).
Yan, Z-M., 1984. Studies on tissue culture of Laminaria japonica and Undaria pinnatifida. Hydrobiologia 116/117: 314–316.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kawashima, Y., Tokuda, H. Regeneration from callus of Undaria pinnatifida (Harvey) Suringar (Laminariales, Phaeophyta). Hydrobiologia 260, 385–389 (1993). https://doi.org/10.1007/BF00049045
Issue Date:
DOI: https://doi.org/10.1007/BF00049045