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Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

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Abstract

Transgenic potato plants, cultivar Désirée, were produced that contained the coat protein gene of potato leafroll luteovirus (PLRV). The transformed potato plants expressed the PLRV coat protein (CP) RNA sequences but accumulation of coat protein in transgenic tissues could not be detected. Upon inoculation with PLRV, the PLRV CP RNA expressing potato plants showed a reduced rate of virus multiplication.

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References

  1. Cadman CH, Harrison BD: Studies on the properties of soil-borne viruses of the tobacco-rattle type occurring in Scotland. Ann Appl Biol 47: 542–556 (1959).

    Google Scholar 

  2. Cuozzo M, O'Conell KM, Kaniewski W, Fang RX, Chua NH, Tumer NE: Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/technology 6: 549–557 (1988).

    Google Scholar 

  3. van Dun CMP, Bol JF, van Vloten-Doting L: Expression of alfalfa mosaic virus and tobacco rattle virus coat protein genes in transgenic tobacco plants. Virology 159: 229–305 (1987).

    Google Scholar 

  4. van Dun CMP, Bol JF: Transgenic tobacco plants accumulating tobacco rattle virus coat protein resist infection with tobacco rattle virus and pea early browning virus. Virology 167: 649–652 (1988).

    Google Scholar 

  5. van Dun CMP, Overduin B, van Vloten-Doting L, Bol JF: Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection. Virology 164: 383–389 (1988).

    Google Scholar 

  6. Gibson RW, Jones MGK, Fish N: Resistance to potato leafroll virus and potato virus Y in somatic hybrids between dihaploidSolanum tuberosum andS. brevidens. Theor Appl Genet 76: 113–117 (1988).

    Google Scholar 

  7. Harrison BD: Potato leafroll virus. CMI/AAB Descriptions of plant viruses, 291 (1984).

  8. Hemenway C, Fang RX, Kaniewski W, Chua NH, Tumer NE: Analysis of the mechanism of protection in transgenic plants expressing the potato virus X coat protein or its antisense RNA. EMBO J 7: 1273–1280 (1988).

    Google Scholar 

  9. van den Heuvel JFJM, Peters D: Improved detection of potato leafroll virus in plant material and in aphids. Phytopathology 79: 963–967 (1989).

    Google Scholar 

  10. Hoekema A, Huisman MJ, Molendijk L, van den Elzen PJM, Cornelissen BJC: The genetic engineering of two commercial potato cultivars for resistance to potato virus X. Bio/technology 7: 273–278 (1989).

    Google Scholar 

  11. Khoury J, Singh RP, Boucher A, Coombs DH: Concentration and distribution of mild and severe strains of potato spindle tuber viroid in cross-protected tomato plants. Phytopathology 78: 1331–1336 (1988).

    Google Scholar 

  12. Kojima N, Lapierre H: Potato leafroll virus. In Smith IM, Dunez J, Philips DH, Leliott RA, Archer SA (eds), European Handbook of Plant Diseases, pp. 23–24, Blackwell Scientific Publications, Oxford (1988).

    Google Scholar 

  13. Kozak M: The scanning model for translation: an update. J Cell Biol 108: 229–241 (1989).

    Google Scholar 

  14. Laemmli UK: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 277: 680–685 (1970).

    Google Scholar 

  15. Lawson C, Kaniewski W, Haley L, Rozman R, Newell C, Sanders P, Tumer NE: Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/technology 8: 127–134 (1990).

    Google Scholar 

  16. Loesch-Fries LS, Merlo D, Zinnen T, Burhop L, Hill K, Krahn K, Jarvis N, Nelson SE, Halk EL: Expression of alfalfa mosaic virus RNA4 in transgenic plants confers virus resistance. EMBO J 6: 1845–1851 (1987).

    Google Scholar 

  17. Mayo MA, Barker H, Robinson DJ, Tamada T, Harrison BD: Evidence that potato leafroll virus is positivestranded, is linked to a small protein and does not contain polyadenylate. J Gen Virol 59: 163–167 (1982).

    Google Scholar 

  18. Mettler IJ: A simple and rapid method for minipreparation of DNA from tissue cultured plant cells. Plant Mol Biol Rep 5: 346–349 (1987).

    Google Scholar 

  19. Miller WA, Waterhouse PM, Gerlach WL: Sequence and organization of barley yellow dwarf virus genomic RNA. Nucl Acids Res 13: 6097–6111 (1988).

    Google Scholar 

  20. Nelson RS, Powell Abel P, Beachy RN: Lesions and virus accumulation in inoculated transgenic tobacco plants expressing the coat protein of tobacco mosaic virus. Virology 158: 126–132 (1987).

    Google Scholar 

  21. Powell PA, Nelson RS, De B, Hoffman N, Rogers SG, Fraley RT, Beachy RN: Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743 (1986).

    Google Scholar 

  22. Powell PA, Sanders PR, Tumer N, Fraley RT, Beachy RN: Protection against tobacco mosaic virus infection in transgenic plants requires accumulation of coat protein rather than coat protein RNA sequences. Virology 175: 124–130 (1990).

    Google Scholar 

  23. Register JC, Beachy RN: Resistance to TMV in transgenic plants results from interference with an early event in infection. Virology 166: 524–532 (1988).

    Google Scholar 

  24. Reinink K, Dieleman FL, Jansen J, Montenarie AM: Interactions between plant and aphid genotypes in resistance of lettuce toMyzus persicae andMacrosiphum euphorbiae. Euphytica 43: 215–222 (1989).

    Google Scholar 

  25. Sherwood JL: Mechanisms of cross-protection between plant virus strains. In: Evered D, Harnett S (eds) Plant Resistance to Viruses, pp. 136–144, John Wiley (1987).

  26. Sijmons PC, Dekker BMM, Schrammeijer B, Verwoerd TC, van den Elzen PJM, Hoekema A: Production of correctly processed human serum albumin in transgenic plants. Bio/technology 8: 217–221 (1990).

    Google Scholar 

  27. Stark DM, Beachy RN: Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/technology 7: 1257–1262 (1989).

    Google Scholar 

  28. Tumer NE, O'Conell KM, Nelson RS, Sanders PR, Beachy RN, Fraley RT, Shah DM: Expression of alfalfa mosaic virus coat protein virus gene confers cross-protection in transgenic tobacco and tomato plants. EMBO J 6: 1181–1188 (1987).

    Google Scholar 

  29. Veidt I, Lot H, Leiser M, Scheidecker D, Guilley H, Richards K, Jonard G: Nucleotide sequence of beet western yellows RNA. Nucl Acids Res 16: 9917–9932 (1988).

    Google Scholar 

  30. Verwoerd TC, Dekker BM, Hoekema A: A small-scale procedure for the rapid isolation of plant RNAs. Nucl Acids Res 17: 2362 (1989).

    Google Scholar 

  31. van der Wilk F, Huisman MJ, Cornelissen BJC, Huttinga H, Goldbach R: Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Lett 245: 51–56 (1989).

    Google Scholar 

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Authors and Affiliations

  1. Research Institute for Plant Protection, P.O. Box 9060, 6700 GW, Wageningen, Netherlands

    Frank van der Wilk & Harm Huttinga

  2. MOGEN International NV, Einsteinweg 97, 2333 CB, Leiden, Netherlands

    Dinie Posthumus-Lutke Willink & Marianne J. Huisman

  3. Department of Virology, Agricultural University Wageningen, P.O. Box 8045, 6700 EM, Wageningen, Netherlands

    Rob Goldbach

Authors
  1. Frank van der Wilk
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  2. Marianne J. Huisman
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  3. Harm Huttinga
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  4. Rob Goldbach
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van der Wilk, F., Willink, D.PL., Huisman, M.J. et al. Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. Plant Mol Biol 17, 431–439 (1991). https://doi.org/10.1007/BF00040637

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  • DOI: https://doi.org/10.1007/BF00040637

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