Abstract
Adventive embryogenesis in vitro-grown somatic cells of Daucus carota L. was increased three-fold by a 45 min plasmolysis pre-treatment using 1M sucrose solutions. A high degree of synchronous development also resulted from this treatment. The enhancement of embryogenesis is interpreted as an increase in the regeneration of cells which have become physiologically somewhat isolated from the tissue of their origin by the plasmolysis-caused rupture of plasmodesmata. Possible causes of the increased synchrony are discussed.
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References
Carr DJ (1976) Plasmodesmata in growth and development. In: Gunning BES, Robards AW (eds) Intercellular communication in plants: Studies on plasmodesmata. Heidelberg: Springer pp 243–290
Dickson H (1932) Polarity and the productions of adventitious growing points in Marchantia polymorpha. Ann Bot 46:683–701
Drake GA, Carr DJ, Anderson WP (1978) Plasmolysis, plasmodesmata, and the electrical coupling of oat coleoptile cells. J Exp Bot 29:1205–1214
Halperin W (1967) Population density effects on embryogenesis in carrot cell cultures Exp Cell Res 48:170–173
Halperin W (1970) Embryos from somatic plant cells. Symp Soc Cell Biol 9:169–191
Halperin W, Jensen WA (1967) Ultrastructural changes during growth and embryogenesis in carrot cell cultures. J Ultrastruc Res 18:428–443
Konar RN, Thomas E, Street HE (1972) Origin and structure of embroids arising from epidermal cells of the stem of Ranunculus sceleratus L. J Cell Sci 11:77–93
Miller JH (1968) Fern gametophytes as experimental material. Bot Rev 34: 361–426
Nagai I (1914) Physiologische Untersuchungen uber Farnprothallien. Flora 106: 281–330
Nagai I (1919) Induced adventitious growth in the gemmae of Marchantia. Bot Mag Tokyo 33:99–109
Narayanaswami S, LaRue CD (1955) The morphogenic effects of various physical factors on the gemmae of Lunularia. Phytomorphology 5:356–372
Price SR (1914) Some studies on the structure of the plant cell by the method of dark-ground illumination. Ann Bot 28:601–632
Schultz R, Jensen WA (1968) Capsella embryogenesis, the early embryo. J Ultrastruct Res 22:376–392
Street HE, Withers LA (1974) The anatomy of embryogenesis in culture. In: Street HE (ed) Tissue culture and plant science. London and New York: Academic Press pp 71–100
Wetherell DF (1969) Phytochrome in cultured wild carrot tissue. I. Synthesis. Plant Physiol 44:1734–1737
Wetherell DF (1978) In vitro embryoid formation in cells derived from somatic plant tissues. In: Hughes K W et al. Propagation of higher plants through tissue culture. Washington: United States Department of Energy pp 102–124
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Wetherell, D.F. Enhanced adventive embryogenesis resulting from plasmolysis of cultured wild carrot cells. Plant Cell Tiss Organ Cult 3, 221–227 (1984). https://doi.org/10.1007/BF00040341
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DOI: https://doi.org/10.1007/BF00040341