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Characterization of Brazilian populations of Pratylenchus brachyurus using morphological and molecular analyses

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Abstract

In Brazil, Pratylenchus brachyurus damages economic important crops and it is managed with non-chemical methods such as crop rotation or resistant cultivars. However, the variability in reproduction and damaging effects observed among P. brachyurus populations could limit the efficacy and long lasting effect of nematode control practices. Twelve populations of P. brachyurus from different geographical areas of Brazil were analyzed morphologically and molecularly. The morphological and morphometric characters of the populations studied were variable, but fitted those of P. brachyurus. Assemblages of populations with different host preferences and reproductive capabilities were revealed by PCA of these populations using selected morphological characters. Phylogenetic analysis using ITS-rDNA sequences distinguished statistically supported groups of sequences that matched those of other populations of P. brachyurus deposited in the GenBank, confirming the identity of populations here studied. Intraspecific sequence diversity was observed among the populations. These variant sequences suggest the presence of microheterogeneity between ITS-1 copies in individuals. The findings of this study did not provide evidence of cryptic species in the P. brachyurus populations studied. The variability within and among populations of P. brachyurus observed in this work must be taken into consideration in breeding programs for resistance to this nematode.

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References

  • Al-Banna L, Williamson V, Gardner SL (1997) Phylogenetic analysis of nematodes of the genus Pratylenchus using nuclear 26S rDNA. Mol Phylogenet Evol 7:94–102

    Article  CAS  PubMed  Google Scholar 

  • Andrés MF, Pinochet J, Hernández-Dorrego A, Delibes A (2000) Detection and analysis of inter- and intraspecific diversity of Pratylenchus spp. using isozyme markers. Plant Pathol 49:640–649

    Article  Google Scholar 

  • Anwar SA, Mckenry MV, Faddoul J (2000) Reproductive variability of field populations of Meloidogyne spp. on grape rootstocks. J Nematol 32:265–270

    CAS  PubMed Central  PubMed  Google Scholar 

  • Boutsika K (2002) Molecular identification and phylogenies of virus and non-virus vector trichidorid nematodes. Ph.D Thesis, University of Dundee. Dundee, UK

  • Café Filho AC, Huang CS (1988) Nematoides do gênero Pratylenchus no Brasil. Fitopatol Bras 13:232–235

    Google Scholar 

  • Campanella JJ, Bitincka L, Smalley J (2003) MatGAT: An application that generates similarity/ identity matrices using protein of DNA sequences. BMC Bioinf 4:29

    Article  Google Scholar 

  • Castillo P, Vovlas N (2007) Pratylenchus (Nematoda: Pratylenchidae): diagnosis, biology, pathogenicity and management. Nematology Monographs and Perspectives, v. 6. Leiden, The Netherlands. Koninklijke Brill NV

  • Coolen WA, D’Herde DJ (1972) A method for the quantitative extraction of nematodes from plant tissue. State Nematology and Entomology Research Station, Ghent

    Google Scholar 

  • Corbett DCM, Clark SA (1983) Surface features in taxonomy of Pratylenchus species. RévNématol 6:85–98

    Google Scholar 

  • De Luca F, Troccoli A, Duncan LW, Subbotin SA, Waeyenberge L, Coyne DL, Brentu FC, Inserra RN (2012) Pratylenchus speijeri n. sp. (Nematoda: Pratylenchidae) a new root-lesion nematode pest of plantain in West Africa. Nematology 14:987–1004

    Article  Google Scholar 

  • Duncan LW, Inserra RN, Thomas WK, Dunn D, Mustika I, Frisse LM, Mendes ML, Morris K, Kaplan T (1999) Molecular and morphological analysis of isolates of Pratylenchus coffeae and closely related species. Nematropica 29:61–80

    Google Scholar 

  • Eisenback J (1985) Techniques for preparing nematodes for scanning electron microscopy. In: Barker KR, Carter CC, Sasser JN (eds) An advanced treatise on Meloidogyne. North Carolina State University, Raleigh, pp 79–105

    Google Scholar 

  • Elbadri GAA, De Ley P, Waeyenberge L, Vierstraete A, Moens M, Vanfleteren J (2002) Intraspecific variation in Radopholus similis isolates assessed with restriction fragment length polymorphism and DNA sequencing of the internal transcribed spacer region of the ribosomal RNA cistron. Int J Parasitol 32:199–205

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J, Churchill GA (1996) A hidden Markov model approach to variation among sites in rate of evolution. Mol Biol Evol 13:93–104

    Article  CAS  PubMed  Google Scholar 

  • Fortuner R, Quénéhervé P (1980) Morphometrical variability in Helicotylenchus Steiner, 1945: influence of the host on H. dihystera (Cobb, 1893) Sher, 1961. Rév Nématol 3:291–296

    Google Scholar 

  • Godfrey GH (1929) A destructive root disease of pineapples and other plants due to Tylenchus brachyurus, n.sp. Phytopathology 7:611–630

    Google Scholar 

  • Goulart AMC (2008) Aspectos gerais sobre nematoides das lesões radiculares (gênero Pratylenchus). Embrapa Cerrados, Planaltina

    Google Scholar 

  • Grewal PS, Richardson PN, Wright DJ (1990) Effects of killing, fixing and mounting methods on taxonomic characters of parthenogenetic adult female Caenorhabditis elegans (Nematoda: Rhabditidae). Rév Nématol 13:437–444

    Google Scholar 

  • Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Jenkins WR (1964) A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Dis Rep 48:692

    Google Scholar 

  • Kaplan M, Caswell-Chen EP, Williamson VM (1999) Assessment of host-induced selection on three geographic isolates of Heterodera schachtii using RAPD and AFLP markers. Phytopathology 89:68–73

    Article  CAS  PubMed  Google Scholar 

  • Lax P, Doucet M, Di Rienzo JM, Pinochet J, Baujard P (2004) Inter-population variability in Pratylenchus vulnus Allen & Jensen, 1951 (Nematoda: Tylenchida). Nematology 6:257–260

    Article  Google Scholar 

  • Loof PAA (1960) Taxonomic studies on the genus Pratylenchus (Nematoda). Tijdschr Over Plantenziekten 66:29–90

    Google Scholar 

  • Machado ACZ, Ferraz LCCB, Inomoto MM (2007a) Response of cotton cultivars to two Brazilian populations of Pratylenchus brachyurus (Godfrey) Filipjev & Sch. Stekh. J Cotton Sci 11:177–181

    Google Scholar 

  • Machado ACZ, Motta LCC, Siqueira KMS, Ferraz LCCB, Inomoto MM (2007b) Host status of green manures for two isolates of Pratylenchus brachyurus in Brazil. Nematology 9:799–805

    Article  Google Scholar 

  • Machado ACZ, Ferraz LCCB, Oliveira CMG (2007c) Development of a species-specific reverse primer for the molecular diagnostic of Pratylenchus brachyurus. Nematropica 37:249–257

    Google Scholar 

  • Mizukubo T (1995) Evidence for Pratylenchus coffeae races in differential reproduction on fifteen cultivars (Nematoda: Pratylenchidae). Jpn J Nematol 25:85–93

    Article  Google Scholar 

  • O’Bannon JH, Tommerlin AT (1970) Pratylenchus as citrus pathogens. Phytopathology 60:1540

    Google Scholar 

  • Oliveira CMG, Hübschen J, Brown DJF, Ferraz LCCB, Wright F, Neilson R (2004) Phylogenetic relationships among Xiphinema and Xiphidorus nematode species from Brazil inferred from 18S rDNA sequences. J Nematol 36:153–159

    CAS  PubMed Central  PubMed  Google Scholar 

  • Olowe T, Corbett DC (1984) Morphology and morphometrics of Pratylenchus brachyurus and P. zeae II. Influence of environmental factors. Indian J Nematol 14:6–17

    Google Scholar 

  • Payan LA, Dickson DW (1988) Host specificity of four populations of Pratylenchus brachyurus. Ann Appl Nematol 2:140–143

    Google Scholar 

  • Payan LA, Dickson DW (1990) Comparison of populations of Pratylenchus brachyurus based on isozyme phenotypes. J Nematol 22:538–545

    CAS  PubMed Central  PubMed  Google Scholar 

  • Pinochet P, Cenis JL, Fernández C, Doucet M, Marull J (1994) Reproductive fitness and random amplified polymorphic DNA variation among isolates of Pratylenchus vulnus. J Nematol 26:271–277

    CAS  PubMed Central  PubMed  Google Scholar 

  • Román J, Hirschmann H (1969) Morphology and morphometrics of six species of Pratylenchus. J Nematol 1:363–386

    PubMed Central  PubMed  Google Scholar 

  • Silva RA, Serrano MAS, Gomes AC, Borges DC, Souza AA, Asmus GL, Inomoto MM (2004) Ocorrência de Pratylenchus brachyurus e Meloidogyne incognita na cultura do algodoeiro no Estado do Mato Grosso. Fitopatol Bras 29:337

    Article  Google Scholar 

  • Siqueira KMS, Inomoto MM (2008) Pathogenicity and reproductive fitness of Pratylenchus brachyurus on cowpea. Nematology 10:495–500

    Article  Google Scholar 

  • Southey JF (1986) Laboratory methods for work with plant and soil nematodes. HM’s Stationery Office, London

    Google Scholar 

  • Stanton JM, McNicol CD, Steele V (1998) Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australas Plant Pathol 27:112–115

    Article  Google Scholar 

  • Strimmer K, Von Haeseler A (1996) Quartet puzzling, a quartet maximum likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969

    Article  CAS  Google Scholar 

  • Subbotin SA, Sturhan D, Chizhov VN, Vovlas N, Baldwin JG (2006) Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rDNA gene sequences. Nematology 8:455–474

    Article  CAS  Google Scholar 

  • Tarjan AC, Frederick JJ (1978) Intraspecific morphological variation among populations of Pratylenchus brachyurus and P. penetrans. J Nematol 10:152–160

    CAS  PubMed Central  PubMed  Google Scholar 

  • Uehara T, Kushida A, Momota Y (1999) Rapid and sensitive identification of Pratylenchus spp. using reverse dot blot hybridization. Nematology 1:549–555

    Article  CAS  Google Scholar 

  • Waeyenberge L, Ryss A, Moens M, Pinochet J, Vrain TC (2000) Molecular characterization of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology 2:135–142

    Article  CAS  Google Scholar 

  • Zijlstra C, Lever AEM, Venk BJ, Van Silfhout CH (1995) Differences between ITS regions of isolates of root-knot nematodes Meloidogyne hapla and M. chitwoodi. Phytopathology 85:1231–1237

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Andressa C. Z. Machado

    Present address: Instituto Agronômico do Paraná, 86047-902, Londrina, PR, Brazil

Authors and Affiliations

  1. Instituto Biológico, 13001-970, Campinas, SP, Brazil

    Andressa C. Z. Machado & Cláudio M. G. Oliveira

  2. Departamento de Entomologia, Fitopatologia e Zoologia Agrícola, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil

    Kércya M. S. Siqueira, Luiz Carlos C. B. Ferraz, Mário M. Inomoto & Rosana Bessi

  3. Instituto Biológico, 04014-002, São Paulo, SP, Brazil

    Ricardo Harakava

Authors
  1. Andressa C. Z. Machado
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  2. Kércya M. S. Siqueira
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  3. Luiz Carlos C. B. Ferraz
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  7. Cláudio M. G. Oliveira
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Correspondence to Andressa C. Z. Machado.

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Section Editor: Rosângela D. L. Oliveira

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Machado, A.C.Z., Siqueira, K.M.S., Ferraz, L.C.C.B. et al. Characterization of Brazilian populations of Pratylenchus brachyurus using morphological and molecular analyses. Trop. plant pathol. 40, 102–110 (2015). https://doi.org/10.1007/s40858-015-0013-0

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  • DOI: https://doi.org/10.1007/s40858-015-0013-0

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