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Bioremediation of dry olive-mill residue removes inhibition of growth induced by this waste in tomato plants

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Abstract

The disposal of dry olive-mill residue, the waste product from olive oil production, is a serious environmental issue. Dry olive-mill residue, being rich in organic and inorganic nutrients, could be used as fertilizer; however, it contains phenolic compounds that can inhibit plant growth. In order to clarify whether bioremediation of this waste could be a valuable strategy for its reuse, the effect of aqueous extract of dry olive-mill residue, untreated or bioremediated by the saprobe fungi Coriolopsis rigida and Penicillium chrysogenum-10, has been analyzed in relation to some physiological parameters of tomato plants. The data show that aqueous dry olive-mill residue significantly reduces the biomass of roots and shoots. In particular, it causes a dramatic reduction in root length, area, and volume as well as in the number of root tips. At an early stage, aqueous dry olive-mill residue also reduces the content of chlorophyll a and b and the efficiency of PS II. The inhibition of growth seems to be due to the increase in phenolic compounds that induce oxidative stress. Interestingly, when plants are treated with aqueous dry olive-mill residue bioremediated by saprobe fungi a decrease in phenolic content and an alleviation of oxidative stress occur. In conclusion, the results show that bioremediation of aqueous dry olive-mill residue is a useful tool to remove most of the inhibiting effects of this waste on plant growth.

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Acknowledgments

This study, funded by the Spanish Ministry of Science and Innovation (project AGL2008-572), was carried out at the Estación Experimental del Zaidín (EEZ) in Spain and the University of Bari in Italy. The authors gratefully acknowledge the assistance provided by the Andalusian regional authority’s Study Extension Programs (13P and JAE), co-funded by the Consejo Superior de Investigaciones Científicas (CSIC) and the European Social Fund, to Mercedes García-Sánchez for her pre-doctoral grant and Elisabet Aranda for her postdoctoral research contract. The authors are thankful to Michael O’Shea for proof-reading the English text.

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Authors and Affiliations

  1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental Del Zaidín (EEZ), Consejo Superior de Investigaciones Científicas (CSIC), Prof. Albareda 1 Apdo. 419, 18008, Granada, Spain

    M. García-Sánchez, I. García-Romera & E. Aranda

  2. Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70125, Bari, Italy

    A. Paradiso & M. C. de Pinto

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  1. M. García-Sánchez
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  2. A. Paradiso
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  3. I. García-Romera
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  4. E. Aranda
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  5. M. C. de Pinto
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Correspondence to M. C. de Pinto.

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García-Sánchez, M., Paradiso, A., García-Romera, I. et al. Bioremediation of dry olive-mill residue removes inhibition of growth induced by this waste in tomato plants. Int. J. Environ. Sci. Technol. 11, 21–32 (2014). https://doi.org/10.1007/s13762-012-0170-3

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  • DOI: https://doi.org/10.1007/s13762-012-0170-3

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