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Myocardial potency of Bio-tea against Isoproterenol induced myocardial damage in rats

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Abstract

Kombucha (Bio-tea) is a beverage produced by the fermentation of sugared black tea using a symbiotic association of bacteria and yeasts. Traditional claims about Kombucha report beneficial effects such as antibiotic properties, gastric regulation, relief from joint rheumatism and positive influence on the cholesterol level, arteriosclerosis, diabetes, and aging problems. The present investigation was carried out to understand the preventive effect of Kombucha on heart weight, blood glucose, total protein, lipid profile and cardiac markers in rats with myocardial damage induced using Isoproterenol. As Bio-tea is produced by fermenting tea, the parameters were compared in rats pre-treated with normal black tea and Bio-tea for 30 days followed by subcutaneous injection of Isoproterenol (85 mg/kg body weight). Normal rats as well as Isoproterenol induced myocardial infarcted rats were also used, which served as controls. Isoproterenol induced myocardial infarcted control rats showed a significant increase in heart weight, blood glucose and cardiac markers and a decrease in plasma protein. Increased levels of cholesterol, triglycerides, low density lipids (LDL) and very low density lipids (VLDL) were also observed, while the high density lipid (HDL) content decreased. Bio-tea showed a higher preventive effect against myocardial infarction when compared to tea, as was observed by the significant reduction in heart weight, and blood glucose and increase in plasma albumin levels. Bio-tea significantly decreased cholesterol, triglycerides, LDL and VLDL while simultaneously increasing the levels of HDL. Similarly a decrease in leakage of cardiac markers from the myocardium was also observed.

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References

  • Adams MR (2002) Prevention of myocardial infarction. Int Med J 32:595–600

    Article  CAS  Google Scholar 

  • Agarwal VK, Basannan DR, Sinha RP, Dutt M, Abraham D, Mustafa MS (2006) Coronary risk factors in a rural community. Indian J Public Health 50:19–23, PMID: 17193754

    Google Scholar 

  • Allen CM (1998) Past research on Kombucha tea. The Kombucha FAQ Part 6. Research and tests results. http://persweb.direct.ca/chaugen/kombucha_faq_part06.html. Accessed 5 January, 2012

  • Anandan R, Mathew S, Sankar TV, Viswanathan Nair PG (2007) Protective effect of n-3 polyunsaturated fatty acids concentrate on isoproterenol induced myocardial infarction in rats. Prostaglandins Leukot Essent Fat Acids 76:153–158

    Article  CAS  Google Scholar 

  • Annoni GG, Bottasso RM, Ciaci D, Donato MF Tripoli A (1982) Clinical Investigation of triglycerides. J Res Lab Med 9:115

    CAS  Google Scholar 

  • Bishop ML, Duben-Engelkirk FEP (2000) Clinical chemistry, 4th edn. Lippincott Williams & Wilkins, Philadelphia

    Google Scholar 

  • Boudina S, Laclau MN, Tariosse L, Daret D, Gouverneur G, Bonoron-Adele S, Saks V, Dos Santos P (2002) Alteration of mitochondrial function in a model of chronic ischemia in vivo in rat heart. Am J Physiol Heart Circ Physiol 282:H821–H831

    Article  CAS  Google Scholar 

  • Buring JE, O’Connor GT, Goldhaber SZ, Rosner B, Herbert PN, Blum CB, Breslow JL, Hennekens CH (1992) Decreased HDL2 and HDL3 cholesterol, Apo-A-I and Apo-A-II, and increased risk of myocardial infarction. Circulation 85:22–29

    Article  CAS  Google Scholar 

  • Burtis CA, Ashwood ER (1999) Tietz textbook of clinical chemistry, 3rd edn. W.B.Saunders Co., Philadelphia

    Google Scholar 

  • Choukroun G, Force T, Hajar R (2002) Mécanisme moléculaire de l’hypertrophie ventriculaire gauche. Flammarion Médecine science, Actualités néphrologiques 221–236

  • Chu SC, Chen C (2006) Effects of origins and fermentation time on the antioxidant activities of Kombucha. Food Chem 98:502–507

    Article  CAS  Google Scholar 

  • De Bono DP, Boon NA (1992) Diseases of the cardiovascular system. In: Edwards CRW, Boucheir IAS, editors. Davidson’s principles of practice and medicine. Hong Kong

  • Doumas BT, Watson WA, Biggs HG (1971) Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chem Acta 31:87–96

    Article  CAS  Google Scholar 

  • Ebenezar KK, Sathish V, Devaki T (2003) Effect of arginine and lysine on mitochondrial function during isoproterenol induced myocardial infarction. Nutr Res 23:1269–1277

    Article  Google Scholar 

  • Esposito K, Nappo F, Marfella R, Giugliano G, Giugliano F, Ciotola M, Quagliaro L, Ceriello A, Giugliano D (2002) Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress. Circulation 106:2067–2072

    Article  CAS  Google Scholar 

  • Flegg HM (1973) Ames award lecture 1972. An investigation of the determination of serum cholesterol by an enzymatic method. Ann Clin Biochem 10:79–84

    Article  CAS  Google Scholar 

  • Friedewald WT, Levy RT, Fredrickson DS (1972) Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502

    CAS  Google Scholar 

  • Gordon T, Castelli WP, Hjortland MC, Kannel WB, Dawber TR (1977) High density lipoprotein as a protective factor against coronary heart disease: the Framingham study. Am J Med 62:707–714

    Article  CAS  Google Scholar 

  • Gornall AG, Bardawill CJ, David MM (1949) Determination of proteins by means of the biuret reaction. J Biol Chem 177:751–766

    CAS  Google Scholar 

  • Gosling P, Beevers DG (1989) Urinary albumin excretion and blood pressure in the general population. Clin Sci 76:39–42

    CAS  Google Scholar 

  • Goyal S, Siddiqui MK, Siddiqui KM, Arora S, Mittal R, Joshi S, Arya DS (2009) Cardioprotective effect of ‘Khamira Abresham Hakim Arshad Wala’ a unani formulation in isoproterenol-induced myocardial necrosis in rats. Exp Toxicol Pathol 62:61–74

    Article  Google Scholar 

  • Heather LC, Catchpole AF, Stuckey DJ, Cole MA, Carr CA, Clarke K (2009) Isoproterenol induces in vivo functional and metabolic abnormalities; similar to those found in the infracted rat heart. J Physiol Pharmacol 3:31–39, PMID: 19826179

    Google Scholar 

  • Ishihara M, Inoue I, Kawagoe T, Shimatani Y, Kurisu S, Nishioka K, Umemura T, Nakamura S, Yoshida M (2003) Impact of acute hyperglycemia on left ventricular function after reperfusion therapy in patients with a first anterior wall acute myocardial infarction. Am Heart J 146:674–678

    Article  CAS  Google Scholar 

  • Jensen CJ, Eberle HC, Nassenstein K, Schlosser T, Farazandeh M, Naber CK, Sabin GV, Bruder O (2011) Impact of hyperglycemia at admission in patients with acuteST-segment elevation myocardial infarction as assessed by contrast-enhanced MRI. Clin Res Cardiol 100:649–659

    Article  CAS  Google Scholar 

  • Katz AM, Messineo FC (1981) Lipid-membrane interactions and the pathogenesis of ischemic damage in the myocardium. Circ Res 48:1–16

    Article  CAS  Google Scholar 

  • Kersten JR, Montgomery MW, Ghassemi T, Gross ER, Toller WG, Pagel PS, Warltier DC (2001) Diabetes and hyperglycemia impair activation of mitochondrial K (ATP) channels. Am J Physiol Heart Circ Physiol 280:H1744–H1750

    CAS  Google Scholar 

  • Lavi S, Kapeliovich M, Gruberg L, Roquin A, Boulos M, Grenadier E, Amikam S, Markiewicz W, Beyar R, Hammerman H (2008) Hyperglycemia during acute myocardial infarction in patients who are treated by primary percutaneous coronary intervention: impact on long-term prognosis. Int J Cardiol 123:117–122

    Article  Google Scholar 

  • Lopez MJB, Garmendia LJR, Aguilar GMD, de Llano AJM, López AC, Fernández AJ (2004) Cardiovascular risk factors in the circadian rhythm of acute myocardial infarction. Rev Esp Cardiol 57:850–858, PMID: 15373991

    Article  Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Luxton R (1999) Clinical biochemistry, 1st edn. Butterworth-Heineman, Oxford

    Google Scholar 

  • Marfella R, Verza M, Coppola L (2005) Myocardial infarction and hyperglycemia. Am J Cardiol 96:1468

    Article  Google Scholar 

  • Mediene-Benchekor S, Brousseau T, Richard F, Benhamamouch S, Amouyel P (2001) Blood lipid concentrations and risk of myocardial infarction. Lance 358:1064–1065

    Article  CAS  Google Scholar 

  • Murray CJ, Lopez AD (1996) The Global Burden of Disease: a comprehensive assessment of mortality and disability from diseases, injuries and risk factors in 1990 and projected to 2020, Cambridge, MA, Harvard School of Public Health, (Global Burden of Disease and Injury Series, vol. I)

  • Nandave M, Ojha SK, Joshi S, Kumari S, Arya DS (2009) Moringa oleifera leaf extract prevents isoproterenol-induced myocardial damage in rats: evidence for an antioxidant, antiperoxidative and cardio protective intervention. J Med Food 12:47–55

    Article  CAS  Google Scholar 

  • Nordin C, Amiruddin R, Rucker L, Choi J, Kohli A, Marantz PR (2005) Diabetes and stress hyperglycemia associated with myocardial infarctions at an urban municipal hospital: prevalence and effect on mortality. Cardiol Rev 13:223–230

    Article  Google Scholar 

  • Prince SM, Kumaran S (2012) Preventive effects of caffeic acid on lipids, lipoproteins and glycoproteins in isoproterenol induced myocardial infarcted rats. Food Res Int 45:155–160

    Article  Google Scholar 

  • Rajadurai M, Stanely Mainzen Prince P (2006) Preventive effect of naringin on lipids, lipoproteins and lipid metabolic enzymes in isoproterenol –induced myocardial infarction in Wistar rats. J Biochem Mol Toxicol 20:191–197

    Article  CAS  Google Scholar 

  • Sasikumar CS, Shyamala Devi CS (2000) Protective effect of Abana®, a poly-herbal formulation, on isoproterenol-induced myocardial infarction in rats. Indian J Pharmacol 32:198–201 URL: http://www.ijp-online.com/temp/IndianJPharmacol323198-1106503_030425.pdf

  • Sharmila ST, Rajadurai M (2012) Preventive Effect Of Bio-Aq On Cardiac Markers, Lipids, And Membrane Bound Enzymes In Isoproterenol—Induced Myocardial Infarction In Rats. Asian J Pharm Clin Res 5:107–113 URL: http://www.ajpcr.com/Vol5Suppl2/941.pdf

  • Shiomi T, Tsutsui H, Ikeuchi M, Matsusaka H, Hayashidani S, Suematsu N, Wen J, Kubot T, Takeshita A (2003) Streptozotocin-induced hyperglycemia exacerbates left ventricular remodeling and failure after experimental myocardial infarction. J Am Coll Cardiol 42:165–172

    Article  CAS  Google Scholar 

  • Sparagna GC, Hickson Bick DL (1999) Cardiac fatty acid metabolism and the induction of apoptosis. Am J Med Sci 318:15–21

    Article  CAS  Google Scholar 

  • Subramanian R, Ramaswamy M, Wasan KM (2003) Role of lipid and lipoprotein metabolizing enzymes in the development of atherosclerosis. Ind J Exp Biol 41:14–25

    CAS  Google Scholar 

  • Sushmakumari S, Varghese A, Muraleedharan D, Menon VP (1990) Protective action of aspirin in experimental myocardial infarction induced by isoproterenol in rats and its effect on lipid peroxidation. Ind J Exp Biol 28:480–485, PMID: 2401523

    Google Scholar 

  • Trinder P (1969) Determination of glucose in blood using glucose oxidase with an alternative oxygen receptor. Ann Clin Biochem 6:24–27

    Article  CAS  Google Scholar 

  • Vlasov YA, Volkov AM (2004) Dependence of the heart weight on the body weight in patients with cardiovascular diseases. Hum Physiol 30:428–434

    Article  Google Scholar 

  • Wang Y, Ji B, Wu W, Wang R, Yang Z, Zhang D, Tian W (2014) Hepatoprotective effects of kombucha tea: identification of functional strains and quantification of functional components. J Sci Food Agric 94(2):265–272

    Article  CAS  Google Scholar 

  • Whellan DJ (2005) Heart failure disease management: implementation and outcomes. Card Rev 13:231–239

    Article  Google Scholar 

  • WHO (2002a) Health situation in south east region. In. World health report. WHO Publications, New Delhi

    Google Scholar 

  • WHO (2002b) Reducing risks, promoting healthy life. In. World health report. WHO Publications, Geneva

    Google Scholar 

  • Williams SB, Goldfine AB, Timimi FK, Ting HH, Roddy MA, Simonson DC, Creager MA (1998) Acute hyperglycemia attenuates endothelium-dependent vasodilation in humans in vivo. Circulation 97:1695–1701

    Article  CAS  Google Scholar 

  • Yeagle PL (1986) Cholesterol and the cell membrane. Biochim Biophys Acta 822:267–287

    Article  Google Scholar 

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  1. Department of Biosciences, Mangalore University, Mangalagangothri, Mangalore, 574199, Karnataka, India

    Reema Orison Lobo & Chandrakala K. Shenoy

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  1. Reema Orison Lobo
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  2. Chandrakala K. Shenoy
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Correspondence to Reema Orison Lobo.

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Lobo, R.O., Shenoy, C.K. Myocardial potency of Bio-tea against Isoproterenol induced myocardial damage in rats. J Food Sci Technol 52, 4491–4498 (2015). https://doi.org/10.1007/s13197-014-1492-6

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  • DOI: https://doi.org/10.1007/s13197-014-1492-6

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