Probiotics and Antimicrobial Proteins

, Volume 5, Issue 4, pp 259-263

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Lactobacillus paracasei DSMZ16671 Reduces Mutans Streptococci: A Short-Term Pilot Study

  • Caterina HolzAffiliated withORGANOBALANCE GmbH
  • , Christiane AlexanderAffiliated withanalyze&realize AG
  • , Christina BalckeAffiliated withORGANOBALANCE GmbH
  • , Margret MoréAffiliated withanalyze&realize AG
  • , Annegret AuingerAffiliated withanalyze&realize AG
  • , Maren BauerAffiliated withBASF New Business GmbHBASF SE
  • , Lauren JunkerAffiliated withBASF Corporation, Fine Chemical and Biocatalysis Research
  • , Jörg GrünwaldAffiliated withanalyze&realize AG
  • , Christine LangAffiliated withORGANOBALANCE GmbH Email author 
    • , Markus PompejusAffiliated withBASF New Business GmbHBASF Corporation, Research Bioactive Materials


Reducing the burden of pathogenic mutans streptococci is a goal of oral health. Lactobacillus paracasei DSMZ16671, even after heat-killing, specifically co-aggregates mutans streptococci in vitro and retains this activity in human saliva. In rats, it reduces mutans streptococcal colonization of teeth and caries scores. This pilot study sought to assess the potential of heat-killed L. paracasei DSMZ16671 (pro-t-action®) to reduce levels of salivary mutans streptococci in humans, using sugar-free candies as a delivery vehicle. A randomized, placebo-controlled, double-blind in vivo study of three groups examined the short-term effect of sugar-free candies containing 0 (placebo), 1, or 2 mg/candy piece of heat-killed L. paracasei DSMZ16671 on the levels of salivary mutans streptococci determined before and after consumption of the candies. The candies were consumed 4 times during 1.5 consecutive days. Compared to the placebo group, the test groups’ saliva had significantly reduced mutans streptococci as an immediate effect. These results suggest the use of heat-killed L. paracasei DSMZ16671 in suckable candies as a method to reduce mutans streptococci in the mouth and, thereby, caries risk. We think this a new concept and strategy for caries prevention and management.


Dental caries Humans Sugar-free candy Lactobacillus pro-t-action Co-aggregation Saliva