Physiology and Molecular Biology of Plants

, Volume 16, Issue 2, pp 177–185

Studies on antidermatophytic activity of waste leaves of Curcuma longa L.

  • Kumar Pankaj Pandey
  • Rohit Kumar Mishra
  • Ahsan Kamran
  • Piyush Mishra
  • A. K. Bajaj
  • Anupam Dikshit
Research Article

DOI: 10.1007/s12298-010-0019-5

Cite this article as:
Pandey, K.P., Mishra, R.K., Kamran, A. et al. Physiol Mol Biol Plants (2010) 16: 177. doi:10.1007/s12298-010-0019-5

Abstract

During antidermatophytic screening of some essential oils, Curcuma longa L. exhibited the strongest antifungal activity, completely inhibiting the mycelial growth of ringworm, caused by the fungi- Microsporum gypseum and Trichophyton mentagrophytes. The essential oil from leaves of Curcuma longa was fungicidal at 2.5 μl/ml at which it tolerated heavy doses of inoculum. The fungicidal activity of the oil was thermostable up to 80 °C and self life up to 24 months in storage. The oil also showed a broad fungitoxic spectrum, inhibiting the mycelial growth of other fungi, viz., Epidermophyton floccosum, M. nanum, T. rubrum, T. violaceum. Moreover, up to 5 % concentration it did not exhibit any adverse effect on mammalian skins. The oil has been formulated in the form of an ointment, 1 % w/v and subjected to topical testing on patients of the Out Patient Department (OPD) at Moti Lal Nehru Medical College, Allahabad. Patients were selected on the basis of KOHpositive results and diagnosed tenia corporis. After the second week of treatment, all patients were KOH- negative. At the end of medication, 75 % of patients recovered completely while 15 % showed significant improvement from the disease. The ointment thus, can be exploited commercially after ongoing successful clinical trials. Relationship of the dermatophytes to the toxicity of the oil vis-a-vis phylogeny using molecular data of the pathogens have also been discussed.

Keywords

AntidermatophyticCurcuma longaessential oilmicrosporum gypseumtenia corporistrichophyton mentagrophytes

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2010

Authors and Affiliations

  • Kumar Pankaj Pandey
    • 1
  • Rohit Kumar Mishra
    • 1
  • Ahsan Kamran
    • 1
  • Piyush Mishra
    • 1
  • A. K. Bajaj
    • 2
  • Anupam Dikshit
    • 1
  1. 1.Biological Product Laboratory, Department of BotanyUniversity of AllahabadAllahabadIndia
  2. 2.Ex-Head, Department of Dermatology and Veneral DiseasesM.L.N. Medical CollegeAllahabadIndia