Abstract
The developmental capacity of mouse embryos in the Chinese SJ-8 Satellite was observed by real time micrography and telecontrol image transmission. Frozen/thawed 4-cell embryos and blastocysts injected with mouse epidemical stem cells were placed in a specially sealed embryonic incubator, and then the incubator was loaded in a space embryonic culture box devised for space-flight. After the satellite launched and arrived at the anticipated orbit, the real time micrography device was opened based on the telecontrol operational technology. Real time micrographs of the mouse embryos were obtained and stored every 3 hours, then the data of images were transmitted at the suitable time. The experiment persisted for 72 hours. The results showed that during space-flight, most mouse embryos cultured in the sealed culture unit kept integrity and natural structure, their location had minor change, but the embryos did not develop. However, the experiment performed on the ground in the same device showed that 4-cell mouse embryos could develop to blastocysts and hatched blastocysts. It may be concluded that the space environment, especially the change of gravity was likely to harm development of the mouse embryo.
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Andras, N., Marina, G., Kristina, V., Richard, B.: Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press, New York (2003)
Andrea, T.B., Carl, L.K., Eric, G.: Alteration in human mononuclear leucocytes following space flight. Immunology 76, 491–497 (1992)
Cao, Y.J., Fan, X.J., Shen, Z., Ma, B.H., Duan, E.K.: Nitric oxide affects preimplantation embryonic development in a rotating wall vessel bioreactor simulating microgravity. Cell Biol. Int. 31, 24–29 (2007)
Izumi-Kurotani, A., Imai, M., Eguchi, H.: Effects of gravity on spicule formation in culture micromeres of sea urchin embryo. Adv. Space Res. 38, 1112–1116 (2006)
Jones, P.H., Watt, F.M.: Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 73, 713–724 (1993)
Kawakami, S., Kashiwagi, K., Furuno, N., Yamashita, M., Kashiwagi, A.: Effects of hypergravity environments on amphibian development, gene expression and apoptosis. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 145, 65–72 (2006)
Kohtaro, K., Akira, Y., Toshimasa, S., Sumihare, N., Satoshi, Y., Tomoichiro, Y., Hiroyoshi, E., Shusaku, Y., Etsuko, A., Tatsuo, S.: Microgravity generated by space flight has little effect on the growth and development of chick embryonic bone. Biological Sciences in Space 9, 82–94 (1995)
Kojma, Y., Sasaki, S., Kubota, Y., Ikeuchi, T., Hayashi, Y., Kohri, K.: Effects of simulated microgravity on mammalian fertilization and preimplantation embryonic development in vitro. Journal of Fertility and Sterility 74, 1142–1147 (2000)
Leibo, S.P.: Fundamental cryobiology of mouse ova and embryos. In: Elliot, K., Whelan, J. (eds) The Freezing of Mammalian Embryos, pp. 69–92. ES/North Holland, Amsterdam, (1977)
Luchuan, L., Jackie, R.B.: Somatic epidermal stem cells can produce multiple cell lineages during development. Stem Cells 20, 21–31 (2000)
Moody, S., Golden, C.: Developmental biology research in space: issues and directions in the era of the International Space Station. Dev. Biol. 228, 1–5 (2000)
Olenev, S.N., Danilov, A.R., Kryuchkova, T.A., Sorokin, L.M.: Effect of weightlessness on some indices of brain development (results of residence of pregnant rats aboard the Cosmos 1514 biosatellite and investigation of subsequent development of their offspring on Earth). Neurosci. Behav. Physiol. 19, 191–195 (1989)
Paula-Lopes, F.F., Chase, C.C., Alkatanani, Y.M., Krininger, C.E., Rivera, R.M., Tekin, S.: Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperature versus hot climate in responses of preimplantation embryos,reproductive tract tissue and lymphocytes to increased culture temperatures. Reproduction 125, 285–294 (2003)
Rambaut, P.C., Johnston, R.S.: Prolonged weightlessness and calcium loss in man. Acta Astronaut. 6, 1113–1122 (1979)
Souza, K.A., Black, S.D., Wassersug, R.J.: Amphibian development in the virtual absence of gravity. Proc. Natl. Acad. Sci. USA. 92, 1975–1978 (1995)
Zheng, X., Zhang, X.P., Wang, J.J., Li, Y.H.: Effects of simulated weightlessness on reproduction in male rats. Space Medicine & Medical Engineering 16, 379–381 (2003)
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Ma, BH., Cao, YJ., Zheng, WB. et al. Real-Time Micrography of Mouse Preimplantation Embryos in an Orbit Module on SJ-8 Satellite. Microgravity Sci. Technol 20, 127–136 (2008). https://doi.org/10.1007/s12217-008-9013-8
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DOI: https://doi.org/10.1007/s12217-008-9013-8