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Encapsulated Hsp70 decreases endotoxin-induced production of ROS and TNFα in human phagocytes

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Cell Stress and Chaperones Aims and scope

An Erratum to this article was published on 28 February 2017

Abstract

Human heat shock protein Hsp70 was experimentally inserted into polyelectrolyte microcapsules. Encapsulated recombinant Hsp70 was studied in terms of its effects on neutrophil apoptosis, the production of reactive oxygen species, and the secretion of tumor necrosis factor alpha by promonocytic THP-1 cells. It was found that encapsulated Hsp70 effectively inhibits neutrophil apoptosis, unlike free exogenous protein used in solution. In THP-1 cells, encapsulated and free Hsp70 reduced LPS-induced tumor necrosis factor alpha production with a similar efficiency. Encapsulated Hsp70 reduces LPS-induced reactive oxygen species production by neutrophils in the course of its release from the microcapsules but not as much as free Hsp70. Thus, the polyelectrolyte microcapsules can be used as containers for the effective delivery of Hsp70 to neutrophils and monocytes to significantly improve the functioning of the innate immune system.

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Acknowledgments

This work was supported by the Russian Science Foundation grant (no. 14-50-00060).

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Str. 32, Moscow, Russian Federation, 119991

    Yurinskaya M.M. & Evgen’ev M.B.

  2. Institute of Cell Biophysics, Russian Academy of Science, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290

    Yurinskaya M.M., Kochetkova O.Yu., Antonova O.Yu., Evgen’ev M.B. & Vinokurov M.G.

  3. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Str. 3, Pushchino, Moscow Region, Russian Federation, 142290

    Kochetkova O.Yu. & Shabarchina L.I.

  4. Hospital of the Pushchino Research Center, Institutskaya Str. 1, Pushchino, Moscow Region, Russian Federation, 142290

    Suslikov A.V.

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  1. Yurinskaya M.M.
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  2. Kochetkova O.Yu.
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  3. Shabarchina L.I.
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  6. Evgen’ev M.B.
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Correspondence to Evgen’ev M.B..

Additional information

Yurinskaya M.M. and Kochetkova O.Yu. contributed equally to this work.

An erratum to this article is available at http://dx.doi.org/10.1007/s12192-017-0777-x.

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M.M., Y., O.Yu., K., L.I., S. et al. Encapsulated Hsp70 decreases endotoxin-induced production of ROS and TNFα in human phagocytes. Cell Stress and Chaperones 22, 163–171 (2017). https://doi.org/10.1007/s12192-016-0743-z

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