Skip to main content
SpringerLink
Log in

Come together, right now….

  • Bits and Bytes
  • Published:
Journal of Cell Communication and Signaling Aims and scope

Abstract

Editing the genome using approaches like TALEN and siRNA are already well tested. The new kid on the block is CRISPR-Cas9. CRISPR-Cas9 is rapidly evolving with impressive refinements for specificity, eliminating off-target effects, and versatility. One can adjust constructs and conditions to produce opposite effects on the genome and for a specific purpose. The nuances of the system, the means to significantly reduce off-targeting, and numerous applications are now emerging rapidly. This B&B commentary looks forward into how the CRISPR-Cas9 tool might serve the CCN field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Aguiar DP, Correo de Farias G, Branco de souse E, de Mattos Coelho-Aguiar J, Lobo JC, Casado PL, Duarte MEL, Abreu JGA Jr (2014) New strategy to control cell migration and metastasis regulated by CCN2/CTGF. Cancer Cell Int 14:61

    Article  PubMed Central  PubMed  Google Scholar 

  • Bedore J, Leask A, Seguin CA (2014) Targeting the extracellular matrix: matricellular proteins regulate cell-extracellular matrix communication with distinct niches of the intervertebral disc. Matrix Biol 37:124–130

    Article  CAS  PubMed  Google Scholar 

  • Boettcher M, McManus MT (2015) Choosing the right tool for the job: RNAi, TALEN, or CRISPR. Mol Cell 58:575–585

    Article  CAS  PubMed  Google Scholar 

  • Corti S, Faravelli I, Cardano M, Conti L (2015) Human pluripotent stem cells as tools for neurodegenerative and neurodevelopmental disease modeling and drug discovery. Expert Opin Drug Discov 10:615–629

    Article  CAS  PubMed  Google Scholar 

  • Curry EL, Moad M, Robson CN, Heer R (2015) Using pluripotent stem cells as a tool for modeling carcinogenesis. World J Stem Cells 7:461–469

    Article  PubMed Central  PubMed  Google Scholar 

  • Gilles AF, Averof M (2015) Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution. EvoDevo 5:43

    Article  Google Scholar 

  • Harrison MM, Jenkins BV, O’Connor-Giles KM, Wildonger J (2014) A CRISPR view of development. Genes Dev 28:1859–1872

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hartenian E, Doench JG (2015) Genetic screens and functional genomics using CRISPR/Cas9 technology. FEBS J 282:1383–1393

    Article  CAS  PubMed  Google Scholar 

  • Hecki D, Charpantier E (2015) Toward whole-transcriptome editing with CRISPR-Cas9. Mol Cell 58:560–562

    Article  Google Scholar 

  • Humphrey SE, Kasinski AL (2015) RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes. J Hematol Oncol 8:31

    Article  PubMed Central  PubMed  Google Scholar 

  • Ishihara J, Umemoto T, Yamato M, Shiratsuchi Y, Takaki S, Petrich BG, Nakauchi H, Eto K, Kitamura T, Okana T (2014) Nov/CCN3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3. Int J Hematol 99:393–406

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kubota S, Takigawa M (2015) Cellular and molecular actions of CCN2/CTGF and its role under physiological and pathological conditions. Clin Sci 128:181–196

    Article  CAS  PubMed  Google Scholar 

  • Li M, Suzuki K, Young Kim N, Liu G-H, Izpisua Belmonte JC (2014) A cut above the rest: targeted genome editing technologies in human pluripotent stem cells. J Biol Chem 289:4594–4599

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Moore JD (2015) The impact of CRISPR-Cas9 on target identification and validation. Drug Discov Today 20:450–457

    Article  CAS  PubMed  Google Scholar 

  • Murphy-Ullrich JE, Sage EH (2014) Revisiting the matricellular concept. Matrix Biol 37:1–14

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Nihongaki Y, Kawano F, Nakajima T, Sato M (2015) Photoactavable CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol. doi:10.1038/nbt.3245

    PubMed  Google Scholar 

  • Ousterout DG, Kabadi AM, Thakore PI, Majoros WH, Reddy TE, Gersbach CA (2015) Multiplex CRISPR/Cas9-based genome editing for corrections of dystrophin mutations that cause Duchenne muscular dystrophy. Nat Commun 6:6244. doi:10.1038/ncomms7244

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Song B, Fan Y, He W, Zhu D, Niu X, Wang D, Ou Z, Luo M, Sun X (2015) Improved hematopoietic differentiation efficiency of gene-corrected Beta-thalassemia induced pluripotent stem cells by CRISPR/Cas9 system. Stem Cells Dev 24:1053–1065

    Article  CAS  PubMed  Google Scholar 

  • Stemberg SH, Doudna JA (2015) Expanding the biologist’s toolkit with CRISPR-Cas9. Mol Cell 58:568–574

    Article  Google Scholar 

  • Wells JE, Howlett M, Cole CH, Kees UR (2015) Deregulated expression of connective tissue growth factor (CTGF/CCN2) is linked to poor outcome in human cancer. Int J Cancer 137:504–511

    Article  CAS  PubMed  Google Scholar 

  • Yan Q, Zhang Q, Yang H, Zou Q, Tang C, Fan N, Lai L (2014) Generation of multi-gene knockout rabbits using the Cas9/gRNA system. Cell Regen 3:12

    Article  Google Scholar 

  • Yang C, Al-Aama J, Stojkovic M, Keavney B, Trafford A, Lako M, Armstrong L (2015) Cardiac disease modeling using induced pluripotent stem cells. Stem Cells. doi:10.1002/stem.207

    PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada

    Herman Yeger

Authors
  1. Herman Yeger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Herman Yeger.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yeger, H. Come together, right now….. J. Cell Commun. Signal. 9, 283–284 (2015). https://doi.org/10.1007/s12079-015-0301-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12079-015-0301-y

Keywords

Search

Navigation