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Optimization of Lactobacillus brevis NS01 Brevicin Production and Its Application in Apple Juice Biopreservation Using Food-Grade Clarifying Agent Silica as a Carrier

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Abstract

Bacteriocins are nowadays recognized as good biopreservatives that are able to contain microbial contaminant development in several food products. In this study, the goat milk isolate Lactobacillus brevis NS01 brevicin activity on common foodborne pathogenic organisms and its characterization, stability, and statistical response surface methodology (RSM) optimization of media components for enhanced production were determined. Its adsorption onto common stabilizing agent silica and applicability in apple juice preservation were also determined. The L. brevis NS01 produced 3.2 kDa common foodborne pathogen inhibitory brevicin having more polar (68.6 %) than non-polar (30.8 %) amino acids. Optimization of growth conditions by RSM demonstrated that glucose, sodium acetate, and triammonium sulfate concentration of 33.41, 8.36, and 2.22 g/L, respectively, increased its production. Brevicin activity was constant over a broad range of pH (3–8) and temperature (20–100 °C) and reached a maximum at pH 6 and 40 °C. Maximum adsorption of brevicin onto silica took place at pH 6–7, 40 °C for 240 min. The carrier silica exhibited significant Fourier transform infrared (FTIR) spectral changes upon brevicin adsorption and presented good biopreservative activity. This study demonstrates the potential large-scale use of brevicin adsorbed silica for shelf life increase and clarification at a same time.

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Acknowledgments

This project was supported by King Saud University, Deanship of Scientific Research, College of Sciences Research Center.

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The authors report no conflicts of interest in this work.

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Authors and Affiliations

  1. Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, 637 408, Tamil Nadu, India

    Senbagam Duraisamy & Senthilkumar Balakrishnan

  2. Department of Microbiology and Botany, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia

    Murugan Kasi & Saleh Al-Sohaibani

  3. Department of Medical Microbiology, Health and Medical Science College, Haramaya University, P.O. Box 235, Harar, Ethiopia

    Senthilkumar Balakrishnan

  4. Department of Science and Humanities, Hindustan Institute of Technology, Coimbatore, 641 032, Tamil Nadu, India

    Gurusamy Ramasamy

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  1. Senbagam Duraisamy
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  2. Murugan Kasi
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  3. Senthilkumar Balakrishnan
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  4. Saleh Al-Sohaibani
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  5. Gurusamy Ramasamy
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Correspondence to Murugan Kasi.

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Duraisamy, S., Kasi, M., Balakrishnan, S. et al. Optimization of Lactobacillus brevis NS01 Brevicin Production and Its Application in Apple Juice Biopreservation Using Food-Grade Clarifying Agent Silica as a Carrier. Food Bioprocess Technol 8, 1750–1761 (2015). https://doi.org/10.1007/s11947-015-1536-6

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  • DOI: https://doi.org/10.1007/s11947-015-1536-6

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