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Extraction of Total Phenolic Content from Garcinia mangostana Linn. hull. I. Effects of Solvents and UV–Vis Spectrophotometer Absorbance Method

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Abstract

The effect of solvents with different polarity on the extraction of total phenolic content from mangosteen hull was investigated. The Folin–Ciocalteu (FC) method, expressed in equivalent to gallic acid concentration was used to quantify total phenolic content. Methanol was the most effective solvent in giving the highest recovery (p < 0.05) of phenolic compounds, compared to ethanol, acetone, isopropanol, ethyl acetate and distilled water. Regression analysis was performed to evaluate the relationship between the direct absorbance method and the FC method for total phenolic contents determination. Strong coefficients of determinations (R 2 ≥ 0.990) were obtained for the absorbance of acetone, ethanol, and methanol extracts suggesting the regression equations obtained can be used to determine the total phenolic contents without using the FC method.

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Correspondence to Nyuk L. Chin.

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Cheok, C.Y., Chin, N.L., Yusof, Y.A. et al. Extraction of Total Phenolic Content from Garcinia mangostana Linn. hull. I. Effects of Solvents and UV–Vis Spectrophotometer Absorbance Method. Food Bioprocess Technol 5, 2928–2933 (2012). https://doi.org/10.1007/s11947-011-0627-2

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