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Dietary Fish Oil Supplements Increase Tissue n-3 Fatty Acid Composition and Expression of Delta-6 Desaturase and Elongase-2 in Jade Tiger Hybrid Abalone

  • Original Article
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Lipids

Abstract

This study was conducted to investigate the effects of fish oil (FO) supplements on fatty acid composition and the expression of ∆6 desaturase and elongase 2 genes in Jade Tiger abalone. Five test diets were formulated to contain 0.5, 1.0, 1.5, 2.0 and 2.5% of FO respectively, and the control diet was the normal commercial abalone diet with no additional FO supplement. The muscle, gonad and digestive glands (DG) of abalone fed with all of the five test diets showed significantly high levels of total n-3 polyunsaturated fatty acid (PUFA), eicosapentaenoic acid (EPA), docosapentaenoic acid n-3 (DPAn-3), and docosahexaenoic acid (DHA) than the control group. In all three types of tissue, abalone fed diet supplemented with 1.5% FO showed the highest level of these fatty acids (P < 0.05). For DPAn-3 the higher level was also found in muscle and gonad of abalone fed diet supplemented with 2% FO (P < 0.05). Elongase 2 expression was markedly higher in the muscle of abalone fed diet supplemented with 1.5% FO (P < 0.05), followed by the diet containing 2% FO supplement. For ∆6 desaturase, significantly higher expression was observed in muscle of abalone fed with diet containing 0.5% FO supplement (P < 0.05). Supplementation with FO in the normal commercial diet can significantly improve long chain n-3 PUFA level in cultured abalone, with 1.5% being the most effective supplementation level.

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Abbreviations

FO:

Fish oil

DG:

Digestive gland

PUFA:

Polyunsaturated fatty acid(s)

EPA:

Eicosapentaenoic acid

DHA:

Docosahexaenoic acid

DPAn-3:

Docosapentaenoic acid, n-3

ALA:

Alpha-linolenic acid

LA:

Linoleic acid

ARA:

Arachidonic acid

MUFA:

Monounsaturated fatty acid(s)

SFA:

Saturated fatty acid(s)

FAME:

Fatty acid methyl ester(s)

RT-PCR:

Real-time polymerase chain reaction

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Acknowledgments

This project was funded by a postgraduate scholarship at Victoria University, Australia. We thank the Great Southern Waters abalone farm, Victoria, Australia for suppling abalone samples and ADAM and AMOS Pty Ltd, SA, Australia for their assistance in formulating the experimental diets. The authors also thank Melrose Laboratories Pty Ltd, Victoria, Australia for the supply of fish oil, and Assoc. Prof. David Cameron-Smith, Deakin University, Victoria, Australia for providing the PCR primers.

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Authors and Affiliations

  1. School of Biomedical and Health Sciences, Victoria University, P.O. Box 14428, MCMC, Melbourne, VIC, 8001, Australia

    Hintsa T. Mateos & Xiao Q. Su

  2. Molecular Nutrition Unit, School of Medicine, Deakin University, Geelong, VIC, 3217, Australia

    Paul A. Lewandowski

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  1. Hintsa T. Mateos
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  2. Paul A. Lewandowski
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  3. Xiao Q. Su
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Correspondence to Xiao Q. Su.

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Mateos, H.T., Lewandowski, P.A. & Su, X.Q. Dietary Fish Oil Supplements Increase Tissue n-3 Fatty Acid Composition and Expression of Delta-6 Desaturase and Elongase-2 in Jade Tiger Hybrid Abalone. Lipids 46, 741–751 (2011). https://doi.org/10.1007/s11745-011-3565-x

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  • DOI: https://doi.org/10.1007/s11745-011-3565-x

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