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Identification and evaluation of antioxidant activities of bamboo extracts

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Forestry Studies in China

Abstract

The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the inhibition activity for peroxidation of linoleic acid, and the reduction power. The methanol-extracts of moso bamboo culms and madake bamboo leaves presented stronger antioxidant activity compared with DPPH scavenging activity. Methanol-extract of moso bamboo culms was further fractionated by different solvents and n-butanol soluble fraction exhibited the most significant activity in the DPPH scavenging assay. The fractionation of n-butanol soluble extract was isolated by silica gel column with gradient mixture solvent of chloroform and methanol. The isolated fractions were directed by the antioxidant activity measured by scavenging the stable DPPH free radical. It was observed that most of the eluted fractions showed the antioxidative activity. Fractions acquired from elution with the mixture solvent of chloroform and methanol (10:1–5:1) showed stronger antioxidant activity than the other fractions.

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Authors and Affiliations

  1. Faculty of Science and Engineering, Shimane University, 690-8504, Matsue, Japan

    Mu Jun, Uehara Tohru & Furuno Takeshi

  2. College of Material Sciences and Technology, Beijing Forestry University, 100083, Beijing, P. R. China

    Li Jianzhang

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  1. Mu Jun
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  2. Uehara Tohru
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  3. Li Jianzhang
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  4. Furuno Takeshi
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Correspondence to Uehara Tohru.

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Mu, J., Uehara, T., Li, J. et al. Identification and evaluation of antioxidant activities of bamboo extracts. For. Stud. China 6, 1–5 (2004). https://doi.org/10.1007/s11632-004-0011-7

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  • DOI: https://doi.org/10.1007/s11632-004-0011-7

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