Summary
In vitro proliferated sentang shoots were cultured onto half-strength Murashige and Skoog (MS) medium containing combinations of 1-naphthyleneacetic acid (NAA) and indole-3-butyric acid (IBA). Sentang shoots were unable to root in the absence of both auxins. A combination of 0.5 mg NAA per and 1 mg IBA per 1 induced the most shoots to form roots. With the addition of 2.5 g activated charcoal per 1 into half-strength MS medium containing 0.5 mg NAA per 1 and 1 mg IBA per 1, roots were more numerous and longer. Substances like gelrite and phloroglucinol and sugar content which would commonly influence in vitro rooting were inhibitory to adventitious root formation of sentang. Maximal rooting of 100% was achieved in “Culture Pack,” made of fluorocarbon polymer film containing charcoal-free medium with 0.5 mg NAA per 1 and 1 mg IBA per 1. Rooted shoots were acclimatized for 4 wk. Overall survival was 80%. These findings suggest the use of Culture Pack as the culture vessels, with 0.5 mg NAA per 1 and 1 mg IBA per 1 in half-stength MS media to effectively induce roots in sentang shoots.
Similar content being viewed by others
References
Anonymous. In vitro multiplication of woody species. Round Table Conf., Gembloux, Belgium, Centre Rech. Agron. Etat: 1–295; 1978.
Bhansali, R. R. Bud culture for shoot multiplication and plantlet formation of Tecomella undulata (Rohida), a woody tree of the arid zone. Trop. Sci. 33:1–8; 1993.
George, E. F.; Sherrington, P. D. Plant propagation by tissue culture. Reading, Berks: Eastern Press; 1984.
Hartmann, H. T.; Kester, D. E. Plant propagation. Principles and practices. 3rd ed. Englewood Cliffs, NJ: Prentice-Hall, Inc.; 1975.
Kamil, H.; Umboh, M. I. J. Root induction of Santalum album by using IBA and NAA. Seameo Biotrop, Indonesia, BIOTROP Spec. Publ. 49:67–73; 1992.
Kyte, L. Plants from test tubes. An introduction to micropropagation. Portland, Oregon: Timber Press; 1987.
Liew, T. K.; Teo, K. H. Multiple shoot production in vitro of the tropical timber tree, sentang (Azadirachta excelsa Linn). HortScience 33:1073–1075; 1998.
Lineberger, R. D. Care and handling of micropropagated plants. URL http://Aggie-Horticulture.tamu.edu/tisscult/microprop/micropro.html. 1997.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Pierik, R. L. M. In vitro culture of higher plants. Dordrecht, The Netherlands: Martinus Nijhoff Publishers; 1987.
Standardi, A.; Romani, F. Effects of some antioxidants on in vitro rooting of apple shoots. HortScience 25:1435–1436; 1990.
Watson, L.; Dallwitz, M. J. The families and flowering plants: descriptions and illustrations. URL http://muse.bio.cornell.edu/delta/1995.
Zar, J. H. Biostatistical analysis. Englewood Cliffs, NJ: Prentice-Hall, Inc.; 1996.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kooi, L.T., Keng, C.L. & Hoe, C.T.K. In vitro rooting of sentang shoots (Azadirachta excelsa L.) and acclimatization of the plantlets. In Vitro Cell.Dev.Biol.-Plant 35, 396–400 (1999). https://doi.org/10.1007/s11627-999-0053-6
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11627-999-0053-6