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Promoting effects of organic medium supplements on the micropropagation of promising ornamental Daphne species (Thymelaeaceae)

  • Micropropagation
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Abstract

Three Daphne species (Thymelaeaceae) were propagated in vitro using media enriched with natural ingredients including coconut water, pineapple pulp, arabinogalactan, chitosan, and conditioned medium containing exudates of the green alga Desmodesmus subspicatus. High vigor of proliferative shoots and enhanced rooting efficiency were obtained. The propagation rate for shoot cultures of Daphne caucasica and Daphne tangutica increased significantly when cultured in the presence of 10 ml L−1 coconut water or 10 ml L−1 pineapple pulp. Addition of 10 ml L−1 pineapple pulp, 10 ml L−1 coconut water, or 20% conditioned medium to the culture medium stimulated organogenesis in D. caucasica. The percentage of rooted shoots in this difficult-to-root species reached 80% in enriched medium. Daphne jasminea plants rooted efficiently on media without growth regulators but supplemented with 10 ml L−1 pineapple pulp or 10 ml L−1 coconut water. Plants of D. caucasica and D. jasminea were successfully acclimatized to greenhouse conditions. Biochemical evaluation of pineapple pulp using thin-layer chromatography revealed the absence of natural auxins. However, the low-molecular-weight fraction (<500 Da) obtained via dialysis significantly stimulated rhizogenesis in each species tested.

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Acknowledgment

This research was partly supported by The Scholarship for the Employees of University of Agriculture in Kraków granted to A.W.

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Authors and Affiliations

  1. Department of Botany and Plant Physiology, Faculty of Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425, Kraków, Poland

    Alina Wiszniewska & Ewa Hanus-Fajerska

  2. Department of Plant Physiology, University of Gdańsk, Piłsudskiego 46, 81-378, Gdynia, Poland

    Krzysztof Grabski & Zbigniew Tukaj

Authors
  1. Alina Wiszniewska
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  2. Ewa Hanus-Fajerska
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  3. Krzysztof Grabski
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  4. Zbigniew Tukaj
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Correspondence to Alina Wiszniewska.

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Editor: Jeffrey Adelberg

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Wiszniewska, A., Hanus-Fajerska, E., Grabski, K. et al. Promoting effects of organic medium supplements on the micropropagation of promising ornamental Daphne species (Thymelaeaceae). In Vitro Cell.Dev.Biol.-Plant 49, 51–59 (2013). https://doi.org/10.1007/s11627-012-9480-x

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  • DOI: https://doi.org/10.1007/s11627-012-9480-x

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