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Three new species of Phomopsis in Gutianshan Nature Reserve in China

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Abstract

Three new species of Phomopsis causing leaf spots on multiple hosts in Gutianshan Nature Reserve are characterized and illustrated. Phylogenetic analyses and morphological characteristics are employed to justify the introduction of the new species. The phylogenetic relationships of the three new species were inferred by analyses based on combined rDNA-internal transcribed spacer (ITS) and partial translation elongation factor 1-alpha (TEF1) gene regions. Comparisons are made with morphologically similar and phylogenetically closely related species.

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References

  • Arnold RH (1967) A canker and foliage disease of yellow birch. Can J Bot 45:783–801

    Article  Google Scholar 

  • Brayford D (1990) Variation in Phomopsis isolates from Ulmus species in the British Isles and Italy. Mycol Res 94:691–697

    Article  Google Scholar 

  • Cai L, Hyde KD, Taylor PWJ, Weir B, Waller J, Abang MM, Zhang ZJ, Yang YL, Phoulivong S, Liu ZY, Prihastuti H, Shivas RG, McKenzie EHC, Johnston PR (2009) A polyphasic approach for studying Colletotrichum. Fungal Divers 39:183–204

    Google Scholar 

  • Cai L, Giraud T, Zhang N, Begerow D, Cai GH, Shivas RG (2011a) The evolution of species concepts and species recognition criteria in plant pathogenic fungi. Fungal Divers 50:121–133. doi:10.1007/s13225-011-0127-8

    Article  Google Scholar 

  • Cai L, Udayanga D, Manamgoda DS, Maharachchikumbura SSN, McKenzie EHC, Guo LD, Liu XZ, Bahkali A, Hyde KD (2011b) The need to carry out re-inventory of plant pathogenic fungi. Trop Plant Pathol 36:205–213. doi:10.1590/S1982-56762011000400001

    Article  Google Scholar 

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Castlebury LA, Farr DF, Rossman AY, Jaklitsch W (2003) Diaporthe angelicae comb. nov., a modern description and placement of Diaporthopsis in Diaporthe. Mycoscience 44:203–208

    Google Scholar 

  • Crous PW (2005) Impact of molecular phylogenetics on the taxonomy and diagnostics of fungi. Bull OEPP/EPPO 35:47–51. doi:10.1111/j.1365-2338.2005.00811.x

    Article  Google Scholar 

  • Crous PW, Gams W, Stalpers JA, Robert V, Stegehuis G (2004) MycoBank: an online initiative to launch mycology into the 21st century. Stud Mycol 50:19–22

    Google Scholar 

  • Cubero OF, Crespo A, Fatehi J, Bridge PD (1999) DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi. Plant Syst Evol 216:243–249. doi:10.1007/BF01084401

    Article  CAS  Google Scholar 

  • Diedicke H (1911) Die Gattung Phomopsis. Ann Mycologici 9:8–35

    Google Scholar 

  • Farr DF, Castlebury LA (2012) Fungal Databases, Systematic Mycology and Microbiology Laboratory, ARS, USDA. http://nt.ars-grin.gov/fungaldatabases/. Retrieved 13 August 2012

  • Farr DF, Castlebury LA, Rossman AY (2002a) Morphological and molecular characterization of Phomopsis vaccinii and additional isolates of Phomopsis from blueberry and cranberry in the eastern United States. Mycologia 94:494–504

    Article  CAS  PubMed  Google Scholar 

  • Farr DF, Castlebury LA, Rossman AY, Putnam ML (2002b) A new species of Phomopsis causing twig dieback of Vaccinium vitis-idaea (lingonberry). Mycol Res 106:745–752. doi:10.1017/S095375620200583X

    Article  Google Scholar 

  • Giraud T, Gladieux P, Gavrilets S (2010) Linking the emergence of fungal plant diseases with ecological speciation. Trends Ecol Evol 25:387–395. doi:10.1016/j.tree.2010.03.006

    Article  PubMed Central  PubMed  Google Scholar 

  • Glienke C, Pereira OL, Stringari D, Fabris J, Kava-Cordeiro V, et al (2011)Endophytic and pathogenic Phyllosticta species, with reference to thoseassociated with Citrus Black Spot. Persoonia 26:47–56

    Google Scholar 

  • González FR (1916) Micromicetos varios de España y de Cerdaña. Trab Mus Nac Ci Nat Ser Bot 9:1–115

    Google Scholar 

  • Grove WB (1930) New or noteworthy fungi. XI [part 2]. J Bot 68:293–297

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hyde KD, Bussaban B, Paulus B, Crous PW, Lee S, McKenzie EHC, Photita W, Lumyong S (2007) Biodiversity of saprobic fungi. Biodivers Conserv 16:17–35

    Article  Google Scholar 

  • Keissler K (1922) Mykologische Mitteil. 1. Nr. 24. Ann Naturhist Mus Wien 35:1–35

    Google Scholar 

  • Kodsueb R, McKenzie EHC, Lumyong S, Hyde KD (2008) Fungal succession on woody litter of Magnolia liliiflora (Magnoliaceae). Fungal Divers 30:55–57

    Google Scholar 

  • Lue JF, Chi PK (1994) Fungal diseases of cultivated medicinal plants in Guangdong province. Guangdong Science & Technology Press, Guangzhou

    Google Scholar 

  • Mostert L, Crous PW, Petrini O (2000) Endophytic fungi associated with shoots and leaves of Vitis vinifera, with specific reference to the Phomopsis viticola complex. Sydowia 52:46–58

    Google Scholar 

  • Mostert L, Crous PW, Kang JC, Phillips AJL (2001a) Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia 93:146–167

    Article  Google Scholar 

  • Mostert L, Kang JC, Crous PW, Denman S (2001b) Phomopsis saccharata sp. nov., causing a canker and die-back disease of Protea repens in South Africa. Sydowia 53:227–235

    Google Scholar 

  • Nylander JAA (2004) MrModeltest v. 2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University

  • Page RDM (1996) TreeView: An application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Petch T (1925) Additions to Ceylon fungi. III. Ann Roy Bot Gard (Peradeniya) 9:313–328

    Google Scholar 

  • Punithalingam E (1973) Two new species of Phomopsis. Trans Br Mycol Soc 60:157–160. doi:10.1016/S0007-1536(87)80132-8

    Article  Google Scholar 

  • Rannala B, Yang Z (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. J Mol Evol 43:304–311

    Google Scholar 

  • Rayner RW (1970) A mycological colour chart. Commonwealth Mycological Institute, Kew

    Google Scholar 

  • Rehner SA, Uecker FA (1994) Nuclear ribosomal internal transcribed spacer phylogeny and host diversity in the coelomycete Phomopsis. Can J Bot 72:1666–1674. doi:10.1139/b94-204

    Article  CAS  Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. doi:10.1093/bioinformatics/btg180

    Article  CAS  PubMed  Google Scholar 

  • Rossman AY, Palm-Hernández ME (2008) Systematics of plant pathogenic fungi: why it matters. Plant Dis 92:1376–1386. doi:10.1094/PDIS-92-10-1376

    Article  Google Scholar 

  • Rossman AY, Farr DF, Castlebury LA (2007) A review of the phylogeny and biology of the Diaporthales. Mycoscience 48:135–144. doi:10.1007/s10267-007-0347-7

    Article  Google Scholar 

  • Saccardo PA (1905) Notae mycologicae. Ann Mycol 3:166

    Google Scholar 

  • Santos JM, Phillips AJL (2009) Resolving the complex of Diaporthe (Phomopsis) species occurring on Foeniculum vulgare in Portugal. Fungal Divers 34:111–125

    Google Scholar 

  • Santos JM, Correia VG, Phillips AJL (2010) Primers for mating-typediagnosis in Diaporthe and Phomopsis: their use in teleomorphinduction in vitro and biological species definition. Fungal Biol114:255–270

    Google Scholar 

  • Silva DN, Talhinhas P, Cai L, Manuel L, Gichuru EK, Loureiro A, Várzea V, Paulo OS, Batista D (2012) Host-jump drives rapid and recent ecological speciation of the emergent fungal pathogen Colletotrichum kahawae. Mol Ecol 21:2655–2670. doi:10.1111/j.1365-294X.2012.05557.x

    Article  PubMed  Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Version 4. Sinauer, Sunderland

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882. doi:10.1093/nar/25.24.4876

    Article  Google Scholar 

  • Traverso JB (1906) Flora Italica Cryptogama. Pars 1: Fungi. Pyrenomycetae. Xylariaceae, Valsaceae, Ceratostomataceae. Rocca S Casciano, Italy 1:1–352

  • Traverso GB, Spessa C (1910) La flora micologica del Portogallo, Saggio. Bol Soc Brot 25:26–187

    Google Scholar 

  • Udayanga D, Liu XZ, McKenzie EHC, Chukeatirote E, Bahkali AH, Hyde KD (2011) The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Divers 50:189–225. doi:10.1007/s13225-011-0126-9

    Article  Google Scholar 

  • Udayanga D, Liu XZ, Crows PW, McKenzie EHC, Chukeatirote E, Hyde KD (2012a) A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis). Fungal Divers 56:157–171. doi:10.1007/s13225-012-0190-9

    Article  Google Scholar 

  • Udayanga D, Liu XZ, McKenzie EHC, Chukeatirote E, Hyde KD (2012b) Multi-locus phylogeny reveals three new species of Diaporthe from Thailand. Cryptogam Mycol 33:295–309

    Article  Google Scholar 

  • Uecker FA (1988) A world list of Phomopsis names with notes on nomenclature, morphology and biology. Mycol Mem 13:1–231

    Google Scholar 

  • van der Aa HA, Noordeloos ME, de Gruyter J (1990) Species conceptsin some larger genera of the coelomycetes. StudMycol 32:3–19

    Google Scholar 

  • Van Niekerk JM, Groenewald JZ, Farr DF, Fourie PH, Halleen F, Crous PW (2005) Reassessment of Phomopsis species on grapevines. Australas Plant Pathol 34:27–39. doi:10.1071/AP04072

    Article  Google Scholar 

  • van Rensburg JCJ, Lamprecht SC, Groenewald JZ, Castlebury LA,Crous PW (2006) Characterisation of Phomopsis spp. associatedwith die-back of rooibos (Aspalathus linearis) in South Africa.Stud Mycol 55:65–74

    Google Scholar 

  • Webber JF, Gibbs JN (1984) Colonization of elm bark by Phomopsis oblonga.Trans Br Mycol Soc 82:348–352

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, pp 315–322

    Google Scholar 

  • Xiang MM, Jiang ZD, Qi PK (2003) Six species of Phomopsis from Guangzhou, China. Mycosystema 22:515–519

    Google Scholar 

  • Zhaxybayeva O, Gogarten JP (2002) Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. Genomics 3:1–15. doi:10.1186/1471-2164-3-4

    Google Scholar 

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Acknowledgments

This work was financed by CASKSCX2-YW-Z-1026 and NSFC 31110103906.

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Authors and Affiliations

  1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Ya-Hui Gao, Wei Sun, Yuan-Ying Su & Lei Cai

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  1. Ya-Hui Gao
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  2. Wei Sun
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  3. Yuan-Ying Su
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  4. Lei Cai
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Correspondence to Lei Cai.

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Ya-Hui Gao and Wei Sun contributed equally to this paper.

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Gao, YH., Sun, W., Su, YY. et al. Three new species of Phomopsis in Gutianshan Nature Reserve in China. Mycol Progress 13, 111–121 (2014). https://doi.org/10.1007/s11557-013-0898-2

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