Skip to main content
Log in

Vesicle-related OsSEC27P enhances H+ secretion in the iron deficient transgenic tobacco root

  • Article
  • Plant Cytology
  • Published:
Chinese Science Bulletin

Abstract

Iron is an essential micronutritional element for plants. In addition to the iron uptake mechanism Strategy I and Strategy II, the vesicle transport process was also found to participate in iron uptake and homeostasis. Herein, a new iron deficiency induced OsSEC27P gene was isolated and investigated in both its localization and its function in transgenic plants. The vesicle-related protein OsSEC27P may play a potential role in enhancing H+ secretion in roots under the iron deficiency conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Guerinot M L, Yi Y. Iron: Nutritious, noxious, and not readily available. Plant Physiol, 1994, 104: 815–820

    Google Scholar 

  2. Eide D, Broderius M, Fett J, et al. A novel iron-regulated metal transporter from plants identified by functional expression in yeast. Proc Natl Acad Sci USA, 1996, 93: 5624–5628

    Article  Google Scholar 

  3. Curie C, Panaviene Z, Loulergue C. Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake. Nature, 2001, 409: 346–349

    Article  Google Scholar 

  4. Marschner H, Von Blanckenburg F. Different strategies in higher plants in mobilization and uptake of iron. J Plant Nutr, 1986, 9: 695–713

    Article  Google Scholar 

  5. Yang X, Huang J, Jiang Y, et al. Cloning and functional identification of two members of the ZIP (Zrt, Irt-like protein) gene family in rice (Oryza sativa L.). Mol Biol Rep, 2009, 36: 281–287

    Article  Google Scholar 

  6. Lanquar V, Lelievre F, Bolte S. Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. EMBO J, 2005, 24: 4041–4051

    Article  Google Scholar 

  7. Xiao H H, Yin L P, Xu X F. The iron-regulated transporter, MbNRAMP1, isolated from Malus baccata is involved in Fe, Mn and Cd trafficking. Ann Bot, 2008, 102: 881–889

    Article  Google Scholar 

  8. Duy D, Wanner G, Meda A R, et al. PIC1, an ancient permease in Arabidopsis chloroplasts, mediates iron transport. Plant Cell, 2007, 19: 986–1006

    Article  Google Scholar 

  9. Han J H, Song X F, Li P, et al. Maize ZmFDR3 localized in chloroplasts is involved in iron transport. Sci China Ser C-Life Sci, 2009, 52: 864–871

    Article  Google Scholar 

  10. Negishi T, Nakanishi H, Yazaki J, et al. cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests that polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots. Plant J, 2002, 30: 83–94

    Article  Google Scholar 

  11. Yin L P, Sun T, Li W. Transcripts and proteome analysis and membrane vesicle trafficking in rice roots under Fe-deficient condition. Prog Nat Sci, 2004, 14: 522–527

    Google Scholar 

  12. Li P, Qi J L, Wang L. Functional expression of MxIRT1, from Malus xiaojinensis, complements an iron uptake deficient yeast mutant for plasma membrane targeting via membrane vesicles trafficking process. Plant Sci, 2006, 171: 52–59

    Article  Google Scholar 

  13. Cao Z S, Kang H G, Zou S B. Application of patch-clamp technique into the study of cell secretion. Prog Biochem Biophys, 1992, 19: 14–18

    Google Scholar 

  14. Sun T, Li P, Xu Y, et al. Non-invasive scanning ion-selective electrode technique and its applications into the research of higher plants. Prog Nat Sci, 2007, 17: 265–269

    Google Scholar 

  15. Xu Y, Sun T, Yin L P. Application of non-invasive microsensing system to simultaneously measure both H+ and O2 fluxes around the pollen tube. J Integr Plant Biol, 2006, 48: 823–831

    Article  Google Scholar 

  16. Lam S K, Siu C L, Hillmer S, et al. Rice SCAMP1 defines clathrin-coated, trans-golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells. Plant Cell, 2007, 19: 296–319

    Article  Google Scholar 

  17. Kuhtreiber W M, Jaffe L F. Detection of extracellular calcium gradients with a calcium-specific vibrating electrode. J Cell Biol, 1990, 110: 1565–1573

    Article  Google Scholar 

  18. Kunkel J G, Cordeiro S, Xu Y, et al. The Use of Non-invasive Ion-selective Microelectrode Techniques for the Study of Plant Development: Plant Electrophysiology Theory and Methods. Berlin, Heidelberg: Springer-Verlag, 2005

    Google Scholar 

  19. Reid B, Nuccitelli R, Zhao M. Non-invasive measurement of bioelectric currents with a vibrating probe. Nat Protocal, 2007, 2: 661–669

    Article  Google Scholar 

  20. Walker E L, Connolly E L. Time to pump iron: Iron-deficiency-signaling mechanisms of higher plants. Curr Opin Plant Biol, 2008, 11: 530–535

    Article  Google Scholar 

  21. Waters M G, Serafini T, Rothman J E. ’Coatomer’: A cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles. Nature, 1991, 349: 248–251

    Article  Google Scholar 

  22. Duden R, Hosobuchi M, Hamamoto S, et al. Yeast beta- and beta′-coat proteins (COP) two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem, 1994, 269: 24486–24495

    Google Scholar 

  23. Aridor M, Bannykh S I, Rowe T, et al. Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport. J Cell Biol, 1995, 131: 875–893

    Article  Google Scholar 

  24. Eugster A, Frigerio G, Dale M, et al. The alpha- and beta′-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol Biol Cell, 2004, 15: 1011–1023

    Article  Google Scholar 

  25. Stagg S M, Gurkan C, Fowler D M, et al. Structure of the Sec13/31 COPII coat cage. Nature, 2006, 439: 234–238

    Article  Google Scholar 

  26. Pimpl P, Movafeghi A, Coughlan S, et al. In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell, 2000, 12: 2219–2236

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to WeiZhong Zhao or LiPing Yin.

Additional information

These authors contributed equally to this work

About this article

Cite this article

Yang, G., Ma, F., Wang, Y. et al. Vesicle-related OsSEC27P enhances H+ secretion in the iron deficient transgenic tobacco root. Chin. Sci. Bull. 55, 3298–3304 (2010). https://doi.org/10.1007/s11434-010-4012-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-010-4012-8

Keywords

Navigation