Abstract
Iron is an essential micronutritional element for plants. In addition to the iron uptake mechanism Strategy I and Strategy II, the vesicle transport process was also found to participate in iron uptake and homeostasis. Herein, a new iron deficiency induced OsSEC27P gene was isolated and investigated in both its localization and its function in transgenic plants. The vesicle-related protein OsSEC27P may play a potential role in enhancing H+ secretion in roots under the iron deficiency conditions.
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Guerinot M L, Yi Y. Iron: Nutritious, noxious, and not readily available. Plant Physiol, 1994, 104: 815–820
Eide D, Broderius M, Fett J, et al. A novel iron-regulated metal transporter from plants identified by functional expression in yeast. Proc Natl Acad Sci USA, 1996, 93: 5624–5628
Curie C, Panaviene Z, Loulergue C. Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake. Nature, 2001, 409: 346–349
Marschner H, Von Blanckenburg F. Different strategies in higher plants in mobilization and uptake of iron. J Plant Nutr, 1986, 9: 695–713
Yang X, Huang J, Jiang Y, et al. Cloning and functional identification of two members of the ZIP (Zrt, Irt-like protein) gene family in rice (Oryza sativa L.). Mol Biol Rep, 2009, 36: 281–287
Lanquar V, Lelievre F, Bolte S. Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. EMBO J, 2005, 24: 4041–4051
Xiao H H, Yin L P, Xu X F. The iron-regulated transporter, MbNRAMP1, isolated from Malus baccata is involved in Fe, Mn and Cd trafficking. Ann Bot, 2008, 102: 881–889
Duy D, Wanner G, Meda A R, et al. PIC1, an ancient permease in Arabidopsis chloroplasts, mediates iron transport. Plant Cell, 2007, 19: 986–1006
Han J H, Song X F, Li P, et al. Maize ZmFDR3 localized in chloroplasts is involved in iron transport. Sci China Ser C-Life Sci, 2009, 52: 864–871
Negishi T, Nakanishi H, Yazaki J, et al. cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests that polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots. Plant J, 2002, 30: 83–94
Yin L P, Sun T, Li W. Transcripts and proteome analysis and membrane vesicle trafficking in rice roots under Fe-deficient condition. Prog Nat Sci, 2004, 14: 522–527
Li P, Qi J L, Wang L. Functional expression of MxIRT1, from Malus xiaojinensis, complements an iron uptake deficient yeast mutant for plasma membrane targeting via membrane vesicles trafficking process. Plant Sci, 2006, 171: 52–59
Cao Z S, Kang H G, Zou S B. Application of patch-clamp technique into the study of cell secretion. Prog Biochem Biophys, 1992, 19: 14–18
Sun T, Li P, Xu Y, et al. Non-invasive scanning ion-selective electrode technique and its applications into the research of higher plants. Prog Nat Sci, 2007, 17: 265–269
Xu Y, Sun T, Yin L P. Application of non-invasive microsensing system to simultaneously measure both H+ and O2 fluxes around the pollen tube. J Integr Plant Biol, 2006, 48: 823–831
Lam S K, Siu C L, Hillmer S, et al. Rice SCAMP1 defines clathrin-coated, trans-golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells. Plant Cell, 2007, 19: 296–319
Kuhtreiber W M, Jaffe L F. Detection of extracellular calcium gradients with a calcium-specific vibrating electrode. J Cell Biol, 1990, 110: 1565–1573
Kunkel J G, Cordeiro S, Xu Y, et al. The Use of Non-invasive Ion-selective Microelectrode Techniques for the Study of Plant Development: Plant Electrophysiology Theory and Methods. Berlin, Heidelberg: Springer-Verlag, 2005
Reid B, Nuccitelli R, Zhao M. Non-invasive measurement of bioelectric currents with a vibrating probe. Nat Protocal, 2007, 2: 661–669
Walker E L, Connolly E L. Time to pump iron: Iron-deficiency-signaling mechanisms of higher plants. Curr Opin Plant Biol, 2008, 11: 530–535
Waters M G, Serafini T, Rothman J E. ’Coatomer’: A cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles. Nature, 1991, 349: 248–251
Duden R, Hosobuchi M, Hamamoto S, et al. Yeast beta- and beta′-coat proteins (COP) two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem, 1994, 269: 24486–24495
Aridor M, Bannykh S I, Rowe T, et al. Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport. J Cell Biol, 1995, 131: 875–893
Eugster A, Frigerio G, Dale M, et al. The alpha- and beta′-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol Biol Cell, 2004, 15: 1011–1023
Stagg S M, Gurkan C, Fowler D M, et al. Structure of the Sec13/31 COPII coat cage. Nature, 2006, 439: 234–238
Pimpl P, Movafeghi A, Coughlan S, et al. In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell, 2000, 12: 2219–2236
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Yang, G., Ma, F., Wang, Y. et al. Vesicle-related OsSEC27P enhances H+ secretion in the iron deficient transgenic tobacco root. Chin. Sci. Bull. 55, 3298–3304 (2010). https://doi.org/10.1007/s11434-010-4012-8
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DOI: https://doi.org/10.1007/s11434-010-4012-8