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Assessment of Airway Distribution of Transnasal Solutions in Mice by PET/CT Imaging

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Abstract

Purpose

Transnasal administration is one of the most common routes for allergen challenge in mouse models of airway diseases. Although this technique is widely used, neither the amount of allergen that reaches the lung nor its airway distribution has been well established. We used positron emission tomography (PET) and computed tomography (CT) to examine the anatomical distribution of a solution containing a tracer immediately after transnasal delivery and to determine the possible influence of age and administered volume.

Procedures

Forty-six female BALB/c mice were divided into three groups according to instillation volume and age: (A) 15 μl, 8–10 weeks old (N = 10), (B) 30 μl, 8–10 weeks old (N = 20), and (C) 30 μl, 32 weeks old (N = 16). Anesthetized animals underwent a dynamic scan in a dedicated small-animal PET scanner immediately after transnasal administration of a solution containing 18FDG. Regions of interest were used to obtain quantitative data. Animals were also imaged with a small-animal CT scanner to obtain complementary anatomical information.

Results

Mean ± SD (5.69 ± 4.51%) of the solution administered reached the lungs in group A, 41.84 ± 8.03% in group B, and 36.65 ± 16.15% in group C. A comparable percentage was delivered to the left and right lungs in all the groups. Analysis of variance revealed a significant difference between the groups in the proportion of the solution that reached the lungs depending on the injection volume (P < 0.001), but not depending on animal age.

Conclusions

In this first report on quantitative imaging by PET and CT in small animals, we confirmed the suitability of the transnasal route with an instilled volume of 30 μl delivering fluids into the lower airways, although only about 40% of the dose reaches the lungs.

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Acknowledgments

This work was supported by grants from Ministerio de Ciencia y Tecnología (TEC2004-07052-C02-01), Ministerio de Sanidad y Consumo (CIBER CB06/01/0079 CIBER CB07/09/0031 and FIS 2001/0598), Ministerio de Industria (CDTEAM Project) and Fundación de Investigación Médica Mutua Madrileña.

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Authors and Affiliations

  1. Unidad de Medicina y Cirugía Experimental, Hospital General Universitario Gregorio Marañón, Dr. Esquerdo, 46, 28007, Madrid, Spain

    ML Soto-Montenegro, JJ Vaquero & M Desco

  2. Servicio de Alergia, Hospital General Universitario Gregorio Marañón, Madrid, Spain

    L Conejero, ML Baeza & JM Zubeldia

Authors
  1. ML Soto-Montenegro
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  2. L Conejero
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  3. JJ Vaquero
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  4. ML Baeza
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  5. JM Zubeldia
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  6. M Desco
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Corresponding author

Correspondence to M Desco.

Additional information

L Conejero was with the Hospital General Universitario Gregorio Marañón. She is now with the Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.

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Soto-Montenegro, M., Conejero, L., Vaquero, J. et al. Assessment of Airway Distribution of Transnasal Solutions in Mice by PET/CT Imaging. Mol Imaging Biol 11, 263–268 (2009). https://doi.org/10.1007/s11307-009-0199-y

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  • DOI: https://doi.org/10.1007/s11307-009-0199-y

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