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Finding loci associated to partial resistance to white pine blister rust in sugar pine (Pinus lambertiana Dougl.).

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Abstract

White pine blister rust (WPBR) is an exotic disease threatening five-needle pines in North America. In spite of its relatively recent introduction, some five-needle pines such as sugar pine (Pinus lambertiana) have developed both complete (major) gene resistance and partial (quantitative) resistance to WPBR. While significant effort has been dedicated to clone and locate the position of the major gene of WPBR resistance in sugar pine, the genetic basis of quantitative resistance remains largely unknown in all Strobus pines. In this work, we took a preliminary approach to identify potential genotype × phenotype associations using the results of long-term survival and symptoms of infection in both experimental and applied breeding populations. Our study found significant associations between several genes and WPBR disease symptoms such as normal active cankers and blights, important symptoms in the development of partial resistance. No significant associations were found with percentage of survival, probably due to the complex inheritance of the disease and long time to infection. With this study, we hope to lay the ground for further genome-wide association studies using large phenotypic data sets in sugar pine and other Strobus pines.

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Acknowledgements

We would like to thank the U.S. Forest Service sugar pine breeding program in California for establishing the Mapping population used in this study. AVL was supported by a UC MEXUS-CONACyT Postdoctoral fellowship.

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Author notes

  1. Alejandra Vázquez-Lobo and Amanda R. De La Torre contributed equally to this work

Authors and Affiliations

  1. Department of Plant Sciences, University of California-Davis, One Shields Avenue, Davis, CA, 95616, USA

    Alejandra Vázquez-Lobo, Amanda R. De La Torre, Pedro J. Martínez-García, Irina Ćalić & David B. Neale

  2. Centro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, 62209, Cuernavaca, Mor, Mexico

    Alejandra Vázquez-Lobo

  3. OD Taxonomy and Phylogeny, Royal Belgian Institute of Natural Sciences, 1000, Brussels, Belgium

    Carl Vangestel

  4. Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT, 06269, USA

    Jill L. Wegzryn

  5. USDA Forest Service, Placerville, Happy Camp, CA, USA

    Deems Burton, Dean Davis, Bohun Kinloch & Detlev Vogler

Authors
  1. Alejandra Vázquez-Lobo
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  2. Amanda R. De La Torre
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Corresponding author

Correspondence to Amanda R. De La Torre.

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The authors declare that they have no conflict of interest.

Additional information

Communicated by S. C. González-Martínez

Data Archiving Statement

SNP sequences will be submitted to Tree Genes database (http://treegenesdb.org) and accession numbers will be supplied once available, prior to final acceptance of the manuscript.

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Vázquez-Lobo, A., De La Torre, A.R., Martínez-García, P.J. et al. Finding loci associated to partial resistance to white pine blister rust in sugar pine (Pinus lambertiana Dougl.).. Tree Genetics & Genomes 13, 108 (2017). https://doi.org/10.1007/s11295-017-1190-4

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