Abstract
The effect of a two-stage cultivation temperature on the production of pullulan synthesized by Aureobasidium pullulans CGMCC1234 was investigated. Pullulan production was affected by temperature; although the optimum temperature for pullulan production was 26°C, the optimal temperature for cell growth was 32°C. Maximum pullulan production was achieved by growing A. pullulans in a first stage of 32°C for 2 days, and then in a second stage of 26°C for 2 days. Pullulan production using these two-stage temperatures significantly increased: about 27.80% (w/w) compared to constant-temperature fermentation (26°C for 4 days). The morphology of the A. pullulans (CGMCC 1234) was also affected by temperature; the lower temperature (26°C) supported unicellular biomass growth. Results of this study indicate that fermentation using two temperature stages is a promising method for pullulan production.
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Acknowledgments
This research was supported by national high technology research and development program of China (863) (No. 2007AA10Z362), National High Technology R. & D. program of China (Grant no. 2006AA10Z333), Jiangsu Provincial Natural Science Foundation (Grant no. BK2008003) and Research Program of State Key Laboratory of Food Science and Technology, Jiangnan University (Project No. SKLF-MB-200,804).
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Wu, S., Chen, H., Jin, Z. et al. Effect of two-stage temperature on pullulan production by Aureobasidium pullulans . World J Microbiol Biotechnol 26, 737–741 (2010). https://doi.org/10.1007/s11274-009-0231-z
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DOI: https://doi.org/10.1007/s11274-009-0231-z