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Isolation and characterization of bacteria from the rhizosphere of wheat

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Abstract

In this study, we isolated bacteria from rhizosphere and endorhizophere of wheat crops of the central region of Argentina. The isolates were phenotypically characterized and the restriction patterns of 16S rDNA (ARDRA) using endonuclease AluI were analysed. Representative isolates were used to evaluate the effect of the inoculation on the growth of wheat under greenhouse conditions. The effects of plant growth-promoting bacteria on wheat plants were studied by evaluating shoot fresh and dry weights and root fresh and dry weights. One native strain increased the shoot and root dry biomass by 23% and 45% respectively. Other strains increased the shoot dry biomass. A 1.5 kb fragment of the 16S rRNA gene of one isolate was sequenced. This isolate showed high identity with different species of Pseudomonas.

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Acknowledgments

The authors wish to thank Dr. Mario Aguilar, from the Universidad de La Plata (Argentina) for his cooperation in the ARDRA performance. Sonia Fischer is member of the Scientific Researcher Career-CONICET (National Council of Technological Researchs). This research was supported by SECYT of the Universidad Nacional de Río Cuarto, PICTO-AGENCIA (n° 01 - 11430) y Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina).

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  1. Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36-km 601, Rio Cuarto, Cordoba, 5800, Argentina

    Sonia E. Fischer, Sandra I. Fischer, Soledad Magris & Gladys B. Mori

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  1. Sonia E. Fischer
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  2. Sandra I. Fischer
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  3. Soledad Magris
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  4. Gladys B. Mori
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Correspondence to Sonia E. Fischer.

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Fischer, S.E., Fischer, S.I., Magris, S. et al. Isolation and characterization of bacteria from the rhizosphere of wheat. World J Microbiol Biotechnol 23, 895–903 (2007). https://doi.org/10.1007/s11274-006-9312-4

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  • DOI: https://doi.org/10.1007/s11274-006-9312-4

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