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Molecular identification of Ageratum enation virus, betasatellite and alphasatellite molecules isolated from yellow vein diseased Amaranthus cruentus in India

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Abstract

Natural occurrence of yellow vein disease on Amaranthus cruentus was observed at Lucknow, India in the year 2008. The causal virus was successfully transmitted through whiteflies (Bemisia tabaci) from diseased A. cruentus to healthy seedlings of A. cruentus and other test species which indicated begomovirus infection. The begomovirus DNA-A, betasatellite, and alphasatellite components associated with yellow vein disease were amplified by rolling circle amplification using Ø-29 DNA polymerase from diseased A. cruentus and characterized by their sequence analyses. The begomovirus DNA-A genome contained six ORFs: AV2 and AV1 in virion sense and AC3, AC2, AC1, and AC4 in complementary sense strand; and a non-translated intergenic region having the conserved geminiviral nonanucleotide sequence. The virus isolate showed 97–99 % sequence identities and close phylogenetic relationships with various isolates of Ageratum enation virus (AgEV); therefore, the isolate under study was identified as AgEV. The beta- and alphasatellite molecules were also identified to be associated with the disease based on their high sequence identities and close phylogenetic relationships with the respective molecules reported worldwide. Co-infiltration of agro-infectious clones of AgEV DNA-A and its betasatellite DNA induced leaf curl and enation symptoms after 25–35 days on A. cruentus, Nicotiana benthamiana, and N. glutinosa plants. We report the association of AgEV, betasatellite and alphasatellite components with yellow vein disease of A. cruentus from India.

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Acknowledgments

The authors are thankful to the Director of the CSIR-National Botanical Research Institute, Lucknow for the facilities and the Department of Biotechnology, New Delhi, India, for fellowship to Ashish Srivastava.

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Authors and Affiliations

  1. Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute (NBRI), Rana Pratap Street, Lucknow, 226001, Uttar Pradesh, India

    Ashish Srivastava, Shri Krishana Raj, Susheel Kumar & Sunil Kumar Snehi

  2. Plant Virology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India

    Aditya Kulshreshtha & Vipin Hallan

  3. Rajiv Gandhi Biotechnology Centre, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, 440033, Maharashtra, India

    Sunil Sohanlal Pande

Authors
  1. Ashish Srivastava
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  2. Shri Krishana Raj
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  3. Susheel Kumar
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  4. Sunil Kumar Snehi
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  5. Aditya Kulshreshtha
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Correspondence to Shri Krishana Raj.

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Srivastava, A., Raj, S.K., Kumar, S. et al. Molecular identification of Ageratum enation virus, betasatellite and alphasatellite molecules isolated from yellow vein diseased Amaranthus cruentus in India. Virus Genes 47, 584–590 (2013). https://doi.org/10.1007/s11262-013-0971-6

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