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Amaranthus leaf mottle virus: 3′-end RNA sequence proves classification as distinct virus and reveals affinities within the genus Potyvirus

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Abstract

Amaranthus leaf mottle virus (AmLMV) was classified as a member of the genus Potyvirus on the basis of its particle morphology, serology, and biological properties (Casetta et al., 1986). Based on these properties, an Amaranthus viridis-infecting virus isolated in Spain, causing mottle and leaf blistering as well as reduced growth has been identified as AmLMV. The 3′ terminal genomic region of this and a reference isolate from Italy has been sequenced and reveals a 95% nucleotide identity between the two isolates. The sequenced part comprises the coat protein with 281 amino acids and 315 nucleotides of the 3′ untranslated region (UTR) preceding a polyadenylated tail. Pairwise comparisons and phylogenetic analysis of the nucleotide and deduced amino acid sequences of the CP and 3′ UTR of the cloned cDNAs with those of other potyviruses shows that AmLMV is a distinct potyvirus closely related to Potato virus Y.

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Acknowledgements

We wish to thank Dr. Esther Giménez-Luque for plant identification and to Drs. Marisol Luis-Arteaga and Antonio Lario for A. deflexus seeds. We also thank Antonia Belmonte and Ana Ortega for their technical assistance.

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Authors and Affiliations

  1. Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (IFAPA-CICE), CIFA La Mojonera, Autovía del Mediterráneo km 420, E-04745, La Mojonera, Almería, Spain

    Eduardo Segundo, Germán Martín, María P. Carmona, Leticia Ruiz, Isabel M. Cuadrado, Leonardo Velasco & Dirk Janssen

  2. Federal Biological Research Center for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11-12, D-38104, Braunschweig, Germany

    Dietrich E. Lesemann

  3. Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politecnica de Valencia, Avenida de los Naranjos s/n, E-46022, Valencia, Spain

    Leticia Ruiz

  4. Seminis Vegetable Seeds Ibérica, PO Box 175, E-04700, El Ejido, Almería, Spain

    Leonardo Velasco

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  1. Eduardo Segundo
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  2. Dietrich E. Lesemann
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  3. Germán Martín
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  4. María P. Carmona
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  5. Leticia Ruiz
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  6. Isabel M. Cuadrado
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Correspondence to Eduardo Segundo.

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Segundo, E., Lesemann, D.E., Martín, G. et al. Amaranthus leaf mottle virus: 3′-end RNA sequence proves classification as distinct virus and reveals affinities within the genus Potyvirus. Eur J Plant Pathol 117, 81–87 (2007). https://doi.org/10.1007/s10658-006-9064-x

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  • DOI: https://doi.org/10.1007/s10658-006-9064-x

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