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Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)

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Abstract

Novel porcine parvoviruses showing the genetic characteristics of bocaviruses have recently been identified. The first such porcine bocavirus (PoBoV1), described as boca-like virus (PBo-likeV), was discovered in PMWS affected pigs in Sweden. Later, several other bocaviruses with divergent genomes were reported under various names in domestic pigs. This is the first report of the presence of bocaviruses in European wild boars. 842 wild boar samples originating from the Western region of Romania (Transylvania) were collected during the 2006/2007 and the 2010/2011 hunting seasons and tested for the presence of PoBoV1 by polymerase chain reaction and sequencing. The results showed 12.94% (109/842) overall positivity, with an increasing prevalence from the 2006/2007 (9.14%, 43/470) to the 2010/2011 (17.74%, 66/372) season (P < 0.01). Differences between the prevalence of the virus in 6–12-month-old-animal (77.06%, 84/109) and 12–36-month-old-animal (22.94%, 25/109) (P < 0.01) indicated that the infection occurred mainly in younger pigs. Comparative sequence analysis of partial VP1/2 genes from wild boars and those available in the GenBank showed only minor differences, indicating that PoBoV1 circulating within the wild boar populations and domestic pigs from different geographic regions were highly similar.

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Acknowledgments

This study was supported by CNCSIS-UEFISCDU, PN II-RU PD-5 189/2010 grant. Research studies of Dr. Cságola were funded by the János Bolyai Research Scholarship of the Hungarian Academy of Science. The authors specially thank the Balassi Institute-Hungarian Scholarship Board Office. The authors thank Dr. Shishan Yuan and Dr. Jinxue Long from the Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences for providing a PoBoV1 positive sample. The authors also thank their colleagues from all Veterinary Authorities across Transylvania for providing the wild boar samples.

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Authors and Affiliations

  1. Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Manastur 3-5, P.O. Box 400372, Cluj-Napoca, Romania

    Dániel Cadar, Timea Kiss & Marina Spînu

  2. Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary

    Attila Cságola, Márta Lőrincz, Kata Tombácz & Tamás Tuboly

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  1. Dániel Cadar
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  2. Attila Cságola
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  3. Márta Lőrincz
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  4. Kata Tombácz
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  5. Timea Kiss
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  6. Marina Spînu
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  7. Tamás Tuboly
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Correspondence to Dániel Cadar.

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Cadar, D., Cságola, A., Lőrincz, M. et al. Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa). Virus Genes 43, 376–379 (2011). https://doi.org/10.1007/s11262-011-0650-4

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