Abstract
This study presents the first evidence of infection by a novel porcine bocavirus (PBoV) in Chinese swine herds. The PCR detection results showed that PBoV was significantly more prevalent in weanling piglets (69.7%, 69/99) with respiratory tract symptoms than that in other samples (0–13.6%) (P < 0.01). Sequence analysis showed that the partial VP1/2 genes were highly conserved (99–100% identity), and only five frequent nucleotide mutation positions existed in Chinese PBoV strains. These data indicate that PBoV might be an emerging virus for swine respiratory tract diseases. This study could help us to better understand the epidemiology of PBoV.
References
Allan GM, Ellis JA (2000) Porcine circoviruses: a review. J Vet Diagn Invest 12:3–14
Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B (2005) Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA 102:12891–12896
Allander T (2008) Human bocavirus. J Clin Virol 41:29–33
Bastien N, Brandt K, Dust K, Ward D, Li Y (2006) Human bocavirus infection, Canada. Emerg Infect Dis 12:848–850
Blomstrom A, Belak S, Fossum C, McKillen J, Allan G, Wallgren P, Berg M (2009) Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. Virus Res 146:125–129
Chae C (2005) A review of porcine circovirus 2-associated syndromes and diseases. Vet J 169:326–336
Choi C, Chae C (2003) Detection of classical swine fever virus in boar semen by reverse transcription–polymerase chain reaction. J Vet Diagn Invest 15:35–41
Ellis JA, Allan GM, Krakowka S (2008) Effect of coinfection with genogroup 1 porcine torque teno virus on porcine circovirus type 2-associated postweaning multisystemic wasting syndrome in gnotobiotic pigs. Am J Vet Res 69:1608–1614
Foulongne V, Olejnik Y, Perez V, Elaerts S, Rodiere M, Segondy M (2006) Human bocavirus in French children. Emerg Infect Dis 12:1251–1253
Fry AM, Lu X, Chittaganpitch M, Peret T, Fischer J, Dowell SF, Anderson LJ, Erdman D, Olsen SJ (2007) Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis 195:1038–1045
Ha Y, Lee YH, Ahn KK, Kim B, Chae C (2008) Reproduction of postweaning multisystemic wasting syndrome in pigs by prenatal porcine circovirus 2 infection and postnatal porcine parvovirus infection or immunostimulation. Vet Pathol 45:842–848
Kekarainen T, Martinez-Guino L, Segales J (2009) Swine torque teno virus detection in pig commercial vaccines, enzymes for laboratory use and human drugs containing components of porcine origin. J Gen Virol 90:648–653
Kupfer B, Vehreschild J, Cornely O, Kaiser R, Plum G, Viazov S, Franzen C, Tillmann RL, Simon A, Muller A, Schildgen O (2006) Severe pneumonia and human bocavirus in adult. Emerg Infect Dis 12:1614–1616
Madec F, Rose N, Grasland B, Cariolet R, Jestin A (2008) Post-weaning multisystemic wasting syndrome and other PCV2-related problems in pigs: a 12-year experience. Transbound Emerg Dis 55:273–283
Manning A, Russell V, Eastick K, Leadbetter GH, Hallam N, Templeton K, Simmonds P (2006) Epidemiological profile and clinical associations of human bocavirus and other human parvoviruses. J Infect Dis 194:1283–1290
Manteufel J, Truyen U (2008) Animal bocaviruses: a brief review. Intervirology 51:328–334
Ouardani M, Wilson L, Jette R, Montpetit C, Dea S (1999) Multiplex PCR for detection and typing of porcine circoviruses. J Clin Microbiol 37(12):3917–3924
Wolf V, Menossi M, Mourao G, Gatti M (2008) Molecular basis for porcine parvovirus detection in dead fetuses. Genet Mol Res 7(2):509–517
Zhou Y, Yu H, Tian Z, Li G, Hao X, Yan L, Peng J, An T, Xu A, Wang Y, Wei T, Zhang S, Cai X, Feng L, Li X, Zhang G, Zhou L, Tong G (2009) Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008. Virus Res 144:136–144
Acknowledgments
This work was partially supported by grants (2006BAD06A01; 2006BAD06A04; 2010-JB05) to S.Y. by the Ministry of Science and Technology (MOST), PR China, and Shanghai Agricultural Funds for “PRRSV surveillance and epidemiology” by Shanghai Municipal Commission of Agriculture. We thank Dr. Tao Tan graciously providing clinical samples.
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Zhai, S., Yue, C., Wei, Z. et al. High prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in China. Arch Virol 155, 1313–1317 (2010). https://doi.org/10.1007/s00705-010-0698-9
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DOI: https://doi.org/10.1007/s00705-010-0698-9