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Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

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Abstract

In this study, using cumin embryo as explant and manipulating plant growth regulators (PGRs) in regeneration medium, the main in vitro morphogenesis pathways including direct shoot organogenesis, direct somatic embryogenesis, indirect somatic embryogenesis, and indirect shoot organogenesis were obtained. The effects of PGRs, subculture, and light on the induction and progression of different pathways were studied in detail. Direct shoot organogenesis occurred on the meristematic zone, while direct somatic embryogenesis was observed on hypocotyl part of cumin embryo (more differentiated part). Application of BAP (0.1 mgl−1) was the sole triggering factor for induction of callus and indirect regeneration pathways. Exogenous IAA played the central role in the direct somatic embryogenesis pathway; however, the combined effects of IAA and NAA along with the high endogenous cytokinin level resulted in direct shoot organogenesis. Subculturing revealed accelerating effects on direct somatic embryogenesis pathway and callus formation. Conversely, subculturing had negative effect on direct shoot organogenesis pathway. In certain combinations of PGRs, like 0.4 mgl−1 IAA + 0.4 mgl−1 NAA, co-induction and co-regeneration of different pathways were observed. Investigation of genotype dependencies of different pathways showed that direct pathways are more genotype-dependent, stable, and faster than indirect pathways. This research presents the embryo of cumin as a convenient model material for induction and comparison of different morphogenesis pathways.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

Indole-3-acetic acid

NAA:

α-naphthaleneacetic acid

PGR:

Plant growth regulator

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Acknowledgments

We would like to greatly thank Mr. Michael Seymour and Miss Tammana Saiyed (La Trobe university, Bundoora, Australia) for editing the manuscript.

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Authors and Affiliations

  1. Department of Crop Production and Plant Breeding, Faculty of Agriculture, University of Shiraz, Shiraz, Iran

    Esmaeil Ebrahimie

  2. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran

    Mohammad Reza Naghavi, Abdolhadi Hosseinzadeh & Mohammad Reza Behamta

  3. Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran

    Manijeh Mohammadi-Dehcheshmeh

  4. Laboratoire de Biotechnologie, et Amelioration des Plantes, Castanet cedex, France

    Ahmad Sarrafi

  5. Plant Biotechnology Centre, Victorian AgriBiosciences Centre, LaTrobe R&D Park, Bundoora, VIC, Australia

    German Spangenberg

Authors
  1. Esmaeil Ebrahimie
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  2. Mohammad Reza Naghavi
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  3. Abdolhadi Hosseinzadeh
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  4. Mohammad Reza Behamta
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  5. Manijeh Mohammadi-Dehcheshmeh
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  6. Ahmad Sarrafi
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  7. German Spangenberg
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Correspondence to Esmaeil Ebrahimie.

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Ebrahimie, E., Naghavi, M.R., Hosseinzadeh, A. et al. Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material. Plant Cell Tiss Organ Cult 90, 293–311 (2007). https://doi.org/10.1007/s11240-007-9269-5

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  • DOI: https://doi.org/10.1007/s11240-007-9269-5

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