Skip to main content
SpringerLink
Log in

Semen cryopreservation of yellow croaker Larimichthys polyactis

  • Research paper
  • Published:
Reviews in Fish Biology and Fisheries Aims and scope Submit manuscript

Abstract

The effects of various extenders and cryoprotectants on movable spermatozoa ratio (MSR), spermatozoa velocity (SV) and duration of spermatozoa motility (DSM) of post-thawed spermatozoa were examined. The MSR, SV and DSM of post-thawed sperm in artificial seminal plasma (ASP) extender were higher than those in marine fish Ringer’s solution (MFRS) extender (P < 0.01) and was not significantly different from that of fresh sperm. No significant differences were observed in the motility parameters between fresh spermatozoa and frozen-thawed spermatozoa cryopreserved with ASP extender supplement 10% EG (ethylene glycol) cryoprotectant. Using the above method, yellow croaker semen was cryopreserved with extender ASP and 10% EG. As a result, at the spermatozoa/egg ratio of 100,000:1, the fertilization rate and hatching rate of the frozen-thawed spermatozoa cryopreserved for 1 week or 1 year in liquid nitrogen (45.7 ± 3.2% and 27.2 ± 5.0% or 37.5 ± 4.4% and 27.2 ± 5.0%) were similar to that of fresh spermatozoa (51.0 ± 3.1% and 36.7 ± 2.2%). There was a small alternation of shape in cryopreserved spermatozoa compared with fresh spermatozoa. In conclusion, the optimal conditions for yellow croaker semen cryopreservation are ASP extender supplement 10% EG cryoprotectant. This is the first report on semen cryopreservation of yellow croaker Larimichthys polyactis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Cabrita E, Sarasquete C, Martínez-Páramo S, Robles V, Beirão J, Pérez-Cerezales S, Harráez MP (2010) Cryopreservation of fish sperm: applications and perspectives. J Appl Ichthyol 26:623–635

    Article  CAS  Google Scholar 

  • Chang YJ (2001) Milt preservation and juvenile production from cryopreserved sperm in flatfish (Pleuronectiformes). PhD thesis, Pukyong National University, Korea, pp 1–216

  • Chang YJ, Chang YJ (2002) Milt properties of four flatfish species and fine structure of their cryopreserved spermatozoa. J Fish Sci Technol 5:87–96

    Article  Google Scholar 

  • Chang YJ, Choi YH, Lim HK, Kho KH (1999a) Cold storage and cryopreservation of grey mullet Mugil cephalus sperm. J Aquacult 12:57–62 (in Korean)

    Article  Google Scholar 

  • Chang YJ, Lim HK, Chang YJ, Kim HS (1999b) Sperm cryopreservation and fertility of post-thaw sperm in river puffer Takifugu obscrus. J Aquacult 12:1–5 (in Korean)

    Google Scholar 

  • Chao NH, Liao IC (2001) Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture 197:161–189

    Article  Google Scholar 

  • Chao NH, Chen HP, Liao IC (1975) Study on cryogenic preservation of grey mullet sperm. Aquaculture 5:389–406

    Article  CAS  Google Scholar 

  • Chen SL, Tian YS (2005) Cryopreservation of flounder Paralichthys olivaceus embryos by vitrification. Theriogenology 63:1207–1219

    Article  PubMed  CAS  Google Scholar 

  • Chen SL, Ji XS, Yu GC, Tian YS, Sha ZX (2004) Cryopreservation of sperm from turbot Scophthalmus maximus and application to large-sale fertilization. Aquaculture 236:547–556

    Article  Google Scholar 

  • Cloud J, Patton S (2009) Basic principles of fish spermatozoa cryopreservation. In: Cabrita E, Robles V, Herráez P (eds) Methods in reproductive aquaculture: marine and freshwater species. CRC Press, Taylor and Francis Group, pp 237–250

  • Dearnno C, Suquet M, Quemener L, Cosson J, Fierville F, Normant Y, Billard R (1997) Cryopreservation of turbot Scophthamus maximus spermatozoa. Theriogenology 48:589–603

    Article  Google Scholar 

  • Fauvel C, Suquet M, Dreanno C, Zonno V, Menu B (1998) Cryopreservation of sea bass Dicentrarchus labrax spermatozoa in experimental and production simulating conditions. Aquat Living Resour 11:387–394

    Article  Google Scholar 

  • Gwo JC, Arnold CR (1992) Cryopreservation of Atlantic croaker spermatozoa: evaluation of morphological changes. J Exp Zool 264:444–453

    Article  PubMed  CAS  Google Scholar 

  • He SY, Curry Woods III L (2004) Changes in motility, ultrastructure, and fertilization capactity of striped bass Morone saxatilis spermatozoa following cryopreservation. Aquaculture 236:677–686

    Article  CAS  Google Scholar 

  • Jamieson BGM (1991) Fish evolution and systematics: evidssence from spermatozoa. Cambridge University Press, New York

    Google Scholar 

  • Kho KH, Kang KH (2003) Cryopreservation of pointhead flounder Cleisthenes pinetorum herzensteini sperm. Kor J Ichthyol 15:213–218

    Google Scholar 

  • Kim SA, Jung SG, Zhang CI (1997) The effect of seasonal anomalies of seawater temperature and salinity on the fluctuation in yields of yellow croaker Larimichthys polyactis in the Yellow Sea. Fish Ocenogr 6:1–9

    Article  Google Scholar 

  • Kopeika E, Kopeika J, Zhang T (2007) Cryopreservation and freeze-drying protocols. Meth Mol Biol 368:203–217

    Article  CAS  Google Scholar 

  • Kusuda S (2004) Current status and perspective of cryopreservation of sperm and blastomeres in fish. Fish Gen Breed Sci 34:1–25 (in Japanese)

    Google Scholar 

  • Lahnsteiner F (2000) Semen cryopreservation in the Salmonidae and in the Northern pike. Aquacult Res 31:245–258

    Article  Google Scholar 

  • Lahnsteiner F, Weismann T, Patzner RA (1992) Fine structural changes in spermatozoa of the grayling Thymallus thymallus during routine cryopreservation. Aquaculture 103:73–84

    Article  Google Scholar 

  • Lahnsteiner F, Berger B, Weismann T, Patzner RA (1996) Physiological and biochemical determination of rainbow trout Oncorhynchus mykiss semen quality for cryopreservation. J Appl Aquacult 6:47–73

    Article  Google Scholar 

  • Lahnsteiner F, Mansour N, Weismann T (2002) Cryopreservation of spermatozoa of the burbot Lota lota. Cryobiology 45:195–203

    Article  PubMed  CAS  Google Scholar 

  • Le MH (2008) Milt properties and sperm cryopreservation in filefish Thamnaconus modestus. Msc. thesis, Pukyong National University, Korea, pp 1–34

  • Le MH, Lim HK, Min BH, Son MH, Lee JU, Chang YJ (2008) Diluents and cryoprotectants for cryopreservation of filefish Thamnaconus modestus sperm. J Aquacult 21:54–59

    CAS  Google Scholar 

  • Le MH, Lim HK, Min BH, Lee JU, Chang YJ (2011) Semen properties and spermatozoon structure of yellow croaker Larimicthys polyactis. Israeli J Aquacult Bamidgeh 63 (accepted)

  • Lim HK, Chang YJ (1998) Effects of diluents and cryoprotectants on cryoprservation of black porgy Acanthopagrus schlegeli sperm. J Aquacult 11:67–75 (in Korean)

    Google Scholar 

  • Lim HK, An CM, Noh GA, Min BH (2007) Effects of diluents and cryoprotectants on sperm cryopreservation in starry flounder Platichthys stellatus. J Aquacult 20:173–177 (in Korean)

    CAS  Google Scholar 

  • Lim HK, Lee CH, Min BH, Lee JU, Lee CS, Seong KB, Lee SM (2008) Effects of diluents and cryoprotectants on sperm cryopreservation of masou salmon Oncorhynchus masou masou. J Kor Fish Soc 41:267–271 (in Korean)

    CAS  Google Scholar 

  • Lim HK, Le MH, An CM, Kim SY, Park MS, Chang YJ (2010) Reproductive cycle of yellow croaker Larimichthys polyactis in southern waters off Korea. Fish Sci 76:971–980

    Article  CAS  Google Scholar 

  • Richardson GF, Wilson CE, Crim LW, Yao Z (1999) Cryopreservation of yellow tail flounder Pleuronectes platessa semen large straws. Aquaculture 174:89–94

    Article  CAS  Google Scholar 

  • Suquet M, Dorange G, Omnes MH, Normant Y, Le Roux A, Fauvel C (1993) Composition of the seminal fluid and ultrastructure of the spermatozoa of turbot Scophthalmus maximus. J Fish Biol 42:509–516

    Article  Google Scholar 

  • Suquet M, Dreanno C, Fauvel C, Cosson J, Billard R (2000) Cryopreservation of sperm in marine fish. Aquacult Res 31:231–243

    Article  Google Scholar 

  • Yao Z, Crim LW, Richardson GF, Emerson CJ (2000) Motility, fertility and ulstrastructural changes of ocean pout Macrozoarces americanus sperm after cryopreservation. Aquaculture 181:361–375

    Article  Google Scholar 

  • Zhang YZ, Zhang SC, Liu XZ, Xu YY, Wang CL, Sawant MS, Li J, Chen SL (2003) Cryopreservation of flounder Paralichthys olivaceus sperm with a practical methodology. Theriogenology 60:989–996

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Dr. Marc Suquet (IFREMER, France) for his helpful comments, stimulating discussion and critical reading of the manuscript. This research was supported in part by project from National Fisheries Research and Development Institute.

Author information

Authors and Affiliations

  1. Department of Aquaculture, Pukyong National University, Daeyeon-3-Dong, Nam-Gu, Busan, 608-737, Korea

    M. H. Le & Y. J. Chang

  2. Aquaculture Management Division, National Fisheries Research and Development Institute, Busan, 619-902, Korea

    H. K. Lim, B. H. Min & M. W. Park

Authors
  1. M. H. Le
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. K. Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. H. Min
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. W. Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. J. Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. H. Le.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Le, M.H., Lim, H.K., Min, B.H. et al. Semen cryopreservation of yellow croaker Larimichthys polyactis . Rev Fish Biol Fisheries 21, 789–797 (2011). https://doi.org/10.1007/s11160-011-9209-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11160-011-9209-7

Keywords

Search

Navigation