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Bioactivity of Different Enriched Phenolic Extracts of Wild Fruits from Northeastern Portugal: A Comparative Study

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Abstract

Arbutus unedo, Prunus spinosa, Rosa micrantha and Rosa canina are good sources of phenolic compounds, including anthocyanins. These compounds have potent antioxidant properties, which have been related to anticancer activity. Herein, the in vitro antioxidant and antitumor properties of enriched phenolic extracts (non-anthocyanin phenolic compounds enriched extract- PE and anthocyanins enriched extract- AE) of the mentioned wild fruits were evaluated and compared. PE gave higher bioactive properties than the corresponding AE. It was observed a high capacity of A. unedo phenolic extract to inhibit lipid peroxidation in animal brain homogenates (EC50 = 7.21 μg/mL), as also a high antitumor potential against NCI-H460 human cell line (non-small lung cancer; GI50 = 37.68 μg/mL), which could be related to the presence of galloyl derivatives (exclusively found in this species). The bioactivity of the studied wild fruits proved to be more related to the phenolic compounds profile than to the amounts present in each extract, and could be considered in the design of new formulations of dietary supplements or functional foods.

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Abbreviations

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

FBS:

Foetal bovine serum

HBSS:

Hank’s balanced salt solution

SRB:

Sulforhodamine B

TBARS:

Thiobarbituric acid reactive substances

TCA:

Trichloroacetic acid

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Acknowledgments

Foundation for Science and Technology (FCT, Portugal) for PEst-OE/AGR/UI0690/2011, PEst-C/QUI/UI0686/2011, BD/78307/2011 (R. Guimarães), BPD/68344/2010 (R. Calhelha) and researcher contract of L. Barros.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Centro de Investigação de Montanha (CIMO), ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, apartado 1172, 5301-854, Bragança, Portugal

    Rafaela Guimarães, Lillian Barros, Ricardo C. Calhelha, Ana Maria Carvalho & Isabel C. F. R. Ferreira

  2. Centro de Química, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Rafaela Guimarães, Ricardo C. Calhelha & Maria João R. P. Queiroz

Authors
  1. Rafaela Guimarães
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  2. Lillian Barros
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  3. Ricardo C. Calhelha
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  4. Ana Maria Carvalho
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  5. Maria João R. P. Queiroz
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  6. Isabel C. F. R. Ferreira
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Corresponding author

Correspondence to Isabel C. F. R. Ferreira.

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Guimarães, R., Barros, L., Calhelha, R.C. et al. Bioactivity of Different Enriched Phenolic Extracts of Wild Fruits from Northeastern Portugal: A Comparative Study. Plant Foods Hum Nutr 69, 37–42 (2014). https://doi.org/10.1007/s11130-013-0394-5

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  • DOI: https://doi.org/10.1007/s11130-013-0394-5

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