Skip to main content
Log in

Analysis of Flavonoids from Leaves of Cultivated Lycium barbarum L.

  • Original Paper
  • Published:
Plant Foods for Human Nutrition Aims and scope Submit manuscript

Abstract

Leaves of Lycium barbarum are widely used as medicine vegetables and functional tea in China. The main flavonoids present in the leaves were separated and identified by high performance liquid chromatography (HPLC), liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-(APCI) MS) and ultraviolet-visible spectra with shift additives. The predominant flavonoid was identified as rutin. Leaves are the rutin-rich parts (16.03–16.33 mg/g). In the wild and cultivated L. barbarum fruits, contents of rutin were determined very low (0.09–1.38 mg/g). The contents of total flavonoids (21.25 mg/g) of cultivated L. barbarum leaves were much higher than those in the wild L. barbarum leaves (17.86 mg/g), so cultivated barbarum leaves are a suitable source for medicine vegetables and functional tea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. WG (1994) Solanaceae. In: Wu, ZY, PH Raven, eds. Flora of China. Vol. 17 (Verbenaceae through Solanaceae). Science Press, Beijing, and Missouri Botanical Garden Press, USA, pp 300–332

  2. Lin FY, Lai YK, Yu HC, Chen NY, Chang CY, Lo HC, Hsu TH (2008) Effects of Lycium barbarum extract on production and immunomodulatory activity of the extracellular polysaccharopeptides from submerged fermentation culture of Coriolus versicolor. Food Chem 110:446–453

    Article  CAS  Google Scholar 

  3. Ming M, Guanhua L, Zhanhai Y, Guang C, Xuan Z (2009) Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet In Vivo. Food Chem 113:872–877

    Article  Google Scholar 

  4. Qian JY, Liu D, Huang AG (2004) The efficiency of flavonoids in polar extracts of Lycium chinense Mill fruits as free radical scavenger. Food Chem 87:283–288

    Article  CAS  Google Scholar 

  5. Chao JC, Chiang SW, Wang CC, Tsai YH, Wu MS (2006) Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells. World J Gastroenterol 12:4478

    Google Scholar 

  6. Hsu HY, Yang JJ, Ho YH, Lin CC (1999) Difference in the effects of radioprotection between aerial and root parts of Lycium chinense. J Ethnopharmacol 64:101

    Article  CAS  Google Scholar 

  7. Yu MS, Leung SK, Lai SW, Che CM, Zee SY, So KF, Yuen WH, Chang RCC (2005) Neuroprotective effects of anti-aging oriental medicine Lycium barbarum against beta-amyloid peptide neurotoxicity. Exp Gerontol 40(8):716

    Article  Google Scholar 

  8. Gutteridge JM (1993) Free radicals in disease processes: a compilation of cause and consequence. Free Radic Res Commun 19:141–158

    Article  CAS  Google Scholar 

  9. Pérez-Jiménez J, Serrano J, Tabernero M, Arranz S, Díaz-Rubio ME, García-Diz L, Goñi I, Saura-Calixto F (2009) Bioavailability of phenolic antioxidants associated with dietary fiber: plasma antioxidant capacity after acute and long-term intake in humans. Plant Foods Hum Nutr . doi:10.1007/s11130-009-0110-7

    Google Scholar 

  10. Chon SU, Heo BG, Park YS, Kim DK, Gorinstein S (2009) Total phenolics level, antioxidant activities and cytotoxicity of young sprouts of some traditional Korean salad plants. Plant Foods Hum Nutr 64:25–31

    Article  CAS  Google Scholar 

  11. Bystrom LM, Lewis BA, Brown DL, Rodriguez E, Obendorf RL (2009) Phenolics, sugars, antimicrobial and free-radical-scavenging activities of Melicoccus bijugatus Jacq. fruits from the Dominican Republic and Florida. Plant Foods Hum Nutr doi:10.1007/s11130-009-0119-y

  12. Hemwimol S, Pavasant P, Shotipruk A (2006) Ultrasound-assisted extraction of anthraquinones from roots of Morinda citrifolia. Ultrason Sonochem 13:543–548

    Article  CAS  Google Scholar 

  13. Merken HM, Beecher GR (2000) Measurement of food flavonoids by high-performance liquid chromatography: a review. J Agric Food Chem 48:577–599

    Article  CAS  Google Scholar 

  14. Markham KR (1990) Technology of flavonoid identification. Science Press, Beijing

    Google Scholar 

  15. Zhang HF, Yang TS, Li ZZ, Wang Y (2008) Simultaneous extraction of epimedin A, B, C and icariin from Herba Epimedii by ultrasonic technique. Ultrason Sonochem 15:376–385

    Article  CAS  Google Scholar 

  16. Chen ML, Song FR, Guo MQ (2002) Analysis of flavonoids from leaves of Acanthopanax senticosus Harms. Chin J Anal Chem 30:690–694

    CAS  Google Scholar 

  17. Hang L, Yu D (1988) Application of UV spectrometry in organic chemistry. Science Press, Beijing

    Google Scholar 

  18. Fabjan N, Rode J, Kosir IJ, Wang Z, Zhang Z, Kreft I (2003) Tartary buckwheat (Fagopyrum tataricu Gaertn.) as a source of dietary rutin and quercitrin. J Agric Food Chem 51:6452–6455

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This research was partially funded by Knowledge Innovation Project of The Chinese Academy of Sciences (KSCX2-YW-N-030), Major Project of Wuhan Municipal Bureau of Agriculture (200720322099), Special Foundation of President of Chinese Academy of Sciences, 100 Talents Program of Chinese Academy of Sciences, and the CAS/CAFEA International Partnership Program for Creative Research Teams.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Y. Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dong, J.Z., Lu, D.Y. & Wang, Y. Analysis of Flavonoids from Leaves of Cultivated Lycium barbarum L.. Plant Foods Hum Nutr 64, 199–204 (2009). https://doi.org/10.1007/s11130-009-0128-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11130-009-0128-x

Keywords

Navigation