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In-depth proteome analysis of the rubber particle of Hevea brasiliensis (para rubber tree)

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Abstract

The rubber particle is a special organelle in which natural rubber is synthesised and stored in the laticifers of Hevea brasiliensis. To better understand the biological functions of rubber particles and to identify the candidate rubber biosynthesis-related proteins, a comprehensive proteome analysis was performed on H. brasiliensis rubber particles using shotgun tandem mass spectrometry profiling approaches—resulting in a thorough report on the rubber particle proteins. A total of 186 rubber particle proteins were identified, with a range in relative molecular mass of 3.9–194.2 kDa and in isoelectric point values of 4.0–11.2. The rubber particle proteins were analysed for gene ontology and could be categorised into eight major groups according to their functions: including rubber biosynthesis, stress- or defence-related responses, protein processing and folding, signal transduction and cellular transport. In addition to well-known rubber biosynthesis-related proteins such as rubber elongation factor (REF), small rubber particle protein (SRPP) and cis-prenyl transferase (CPT), many proteins were firstly identified to be on the rubber particles, including cyclophilin, phospholipase D, cytochrome P450, small GTP-binding protein, clathrin, eukaryotic translation initiation factor, annexin, ABC transporter, translationally controlled tumour protein, ubiquitin-conjugating enzymes, and several homologues of REF, SRPP and CPT. A procedure of multiple reaction monitoring was established for further protein validation. This comprehensive proteome data of rubber particles would facilitate investigation into molecular mechanisms of biogenesis, self-homeostasis and rubber biosynthesis of the rubber particle, and might serve as valuable biomarkers in molecular breeding studies of H. brasiliensis and other alternative rubber-producing species.

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Abbreviations

REF:

Rubber elongation factor

SRPP:

Small rubber particle protein

CPT:

cis-Prenyl transferase

IPP:

Isopentenyl diphosphate

2-DE:

Two-dimensional electrophoresis

SDS-PAGE:

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

MS:

Mass spectrometry

MS/MS:

Tandem mass spectrometry

SCX:

Strong cation exchange chromatography

MudPIT:

Multidimensional protein identification technology

GeLC–MS:

1D gel and liquid chromatography mass spectrometry

MRM:

Multiple reaction monitoring

LC-MRM-MS:

Liquid chromatography-MRM mass spectrometry

GO:

Gene ontology

Mr:

Relative molecular mass

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of P.R. China (Grant Nos. 31270713 and 30860232), Central Public-interest Scientific Institution Basal Research Fund (Grant Nos. 1630022012020 and 1630022011024) and National Basic Research Program of P.R. China (Grant Nos. 2007CB116203 and 2012CB723005).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou, Hainan, People’s Republic of China

    Longjun Dai, Guijuan Kang, Yu Li, Zhiyi Nie, Cuifang Duan & Rizhong Zeng

  2. State Key Laboratory Incubation Base, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, Hainan, People’s Republic of China

    Longjun Dai, Guijuan Kang, Yu Li, Zhiyi Nie, Cuifang Duan & Rizhong Zeng

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  1. Longjun Dai
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  2. Guijuan Kang
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  3. Yu Li
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  4. Zhiyi Nie
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  5. Cuifang Duan
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Correspondence to Cuifang Duan or Rizhong Zeng.

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Dai, L., Kang, G., Li, Y. et al. In-depth proteome analysis of the rubber particle of Hevea brasiliensis (para rubber tree). Plant Mol Biol 82, 155–168 (2013). https://doi.org/10.1007/s11103-013-0047-y

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