Molecular and Cellular Biochemistry

, Volume 410, Issue 1, pp 165–174

Telopodes of telocytes are influenced in vitro by redox conditions and ageing

Article

DOI: 10.1007/s11010-015-2548-2

Cite this article as:
Enciu, AM. & Popescu, L.M. Mol Cell Biochem (2015) 410: 165. doi:10.1007/s11010-015-2548-2

Abstract

Telocytes (TCs) are a novel cell type identified among interstitial cells in various organs. TCs are characterized by very long cell processes (tens to hundreds micrometres) named telopodes (Tps) with uneven calibre: dilations (podoms) and very thin segments (podomers). However, little is known about the factors which influence Tps conformation. Recently, extracellular matrix proteins were found to influence Tps extension, adherence and spreading. Here, we show that oxidative stress and ageing influence formation of new Tps of TCs cultivated from human non-pregnant myometrium. Using real-time videomicroscopy, we found that ageing the TCs to passage 21 increased the ratio of Tps/TC number with about 50 %, whereas oxidative stress hindered formation of new Tps in both aged and young TCs (passage 7). Under oxidative stress, newly formed cell processes were up to 25 % shorter. Migration pathway length was decreased by 30–40 % for both young and aged cells in an oxidative stress environment. Contrary, addition of N-acetyl cysteine in cell culture medium shifted TCs morphology to a long and slender profile. In conclusion, we showed that TCs specific morphology in vitro is influenced by oxidative status balance, as well as ageing.

Keywords

Telocytes Telopodes Ageing Oxidative stress Cell prolongations N-acetyl cysteine Cell migration 

Supplementary material

Supplementary material 1 (AVI 33759 kb)

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Supplementary material 2 (TIFF 9133 kb)

Supplementary material 3 (AVI 19696 kb)

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Cellular, Molecular Biology and Histology“Carol Davila” University of Medicine and PharmacyBucharest 5Romania
  2. 2.“Victor Babeş” National Institute of PathologyBucharest 5Romania

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