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Structurally Characterized Zn2+ Selective Ratiometric Fluorescence Probe in 100 % Water for HeLa Cell Imaging: Experimental and Computational Studies

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Abstract

Fluorescence recognition of Zn2+ in 100 % aqueous medium using 2-((1, 3 dihydroxy-2-(hydroxymethyl)propan-2 ylimino) methyl) phenol (SALTM) as ratiometric probe is reported. Moreover, SALTM can discriminate Zn2+ from Cd2+ very effectively. The binding constant and detection limit of the probe for Zn2+ is 2.2 × 104 M-1/2 and 2.79 × 10−8 M respectively. Interestingly, corresponding naphthalene derivative (HNTM) having less water solubility fails to be a ratiometric sensor. SALTM can detect intracellular Zn2+ in HeLa cervical cancer cells under fluorescence microscope. Moreover, DFT and TD-DFT studies support experimental findings.

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Acknowledgments

B. Kumari and S. Lohar are thankful to BU and CSIR for fellowship. Assistance from CAS (UGC) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, The University of Burdwan, Burdwan, West Bengal, India

    Babli Kumari, Sisir Lohar, Milan Ghosh, Sabyasachi Ta & Debasis Das

  2. Department of Zoology, Visva Bharati University, Santiniketan, West Bengal, India

    Archya Sengupta, Prajna Paramita Banerjee & Ansuman Chattopadhyay

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  1. Babli Kumari
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  2. Sisir Lohar
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Correspondence to Debasis Das.

Additional information

CCDC numbers of SALTM and HNTM are 992217 and 993440 respectively.

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Kumari, B., Lohar, S., Ghosh, M. et al. Structurally Characterized Zn2+ Selective Ratiometric Fluorescence Probe in 100 % Water for HeLa Cell Imaging: Experimental and Computational Studies. J Fluoresc 26, 87–103 (2016). https://doi.org/10.1007/s10895-015-1688-9

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  • DOI: https://doi.org/10.1007/s10895-015-1688-9

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