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Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy

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Abstract

This study was conducted to explore the anti-inflammatory effect of Jungia sellowii (Asteraceae) using a murine model of pleurisy induced by carrageenan (Cg). This plant is used in southern Brazil to treat inflammatory diseases. J. sellowii leaves were extracted with ethanol/water to obtain the crude extract (CE), which was fractionated with different solvents, yielding n-hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH) fractions, and aqueous fraction (Aq). The major compounds succinic acid (SA) and lactic acid (LA) were isolated from Aq fraction, and their structures were determined by 1H and 13C NMR. Pleurisy was induced by Cg (Saleh et al. 1996). The leukocytes, exudation, myeloperoxidase (MPO) and adenosine–deaminase (ADA) activities, metabolites of nitric oxide (NO x ) levels, protein levels and mRNA expression for interleukin 1 beta (IL-1β), tumour necrosis factor alpha (TNF-α), interleukin 17A (IL17A) and inducible of nitric oxide synthase (iNOs), and p65 protein phosphorylation (NF-κB) were analysed 4 h after pleurisy induction. Animals pre-treated with CE, BuOH, Aq, SA, or LA inhibited leukocytes, exudation, MPO and ADA activities, NO x , IL-1β, TNF-α, and IL-17A levels, and the mRNA expression for IL-1β, TNF-α, IL-17A, iNOS, and p65 protein phosphorylation (NF-κB) (p < 0.05). Our study demonstrated that J. sellowii can protect against inflammation induced by Cg by decreasing the leukocytes and exudation. Its effects are related to the decrease of either proinflammatory cytokines and/or NO x . The isolated compounds SA and LA may play an important role in this anti-inflammatory action by inhibiting all the studied parameters. The anti-inflammatory properties of these compounds are due to the downregulation of NF-κB.

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Acknowledgments

This study was supported by grants from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil, and the Conselho Nacional de Pesquisa e Desenvolvimento (CNPq), Brazil.

Conflict of interest

The authors declare that they have no conflicts of interest to disclose.

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Authors and Affiliations

  1. Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Campus Universitário-Trindade, Florianópolis, Santa Catarina, 88040-970, Brazil

    Marina Nader, Geison Vicente, Julia Salvan da Rosa, Eduardo Monguilhott Dalmarco & Tânia Silvia Fröde

  2. Department of Pharmaceutical Sciences, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Campus Universitário-Trindade, Florianópolis, Santa Catarina, 88040-970, Brazil

    Tamires Cardoso Lima, Alyne Machado Barbosa & Maique Weber Biavatti

  3. Department of Chemistry, Centre of Mathematical Sciences, Federal University of Paraná (UFPR), Curitiba, Paraná, 81531990, Brazil

    Alan Diego Conceição Santos & Andersson Barison

Authors
  1. Marina Nader
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  2. Geison Vicente
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  3. Julia Salvan da Rosa
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  4. Tamires Cardoso Lima
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  5. Alyne Machado Barbosa
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  6. Alan Diego Conceição Santos
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  7. Andersson Barison
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  8. Eduardo Monguilhott Dalmarco
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  9. Maique Weber Biavatti
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  10. Tânia Silvia Fröde
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Correspondence to Tânia Silvia Fröde.

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Nader, M., Vicente, G., da Rosa, J.S. et al. Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy. Inflammopharmacol 22, 351–365 (2014). https://doi.org/10.1007/s10787-014-0210-3

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  • DOI: https://doi.org/10.1007/s10787-014-0210-3

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