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RETRACTED ARTICLE: Membrane-Bound Mucins and Mucin Terminal Glycans Expression in Idiopathic or Helicobacter pylori, NSAID Associated Peptic Ulcers

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This article was retracted on 28 May 2013

Abstract

Background

The ratio of Helicobacter pylori/NSAID-negative gastric ulcers is increasing. Idiopathic gastric ulcers have unique clinical and endoscopic features, and are associated with more bleeding complications and a higher mortality. Alterations in gastric mucin expression and sialylation pattern may be important in ulcer pathogenesis.

Aims

The purpose of this study was to determine the expression pattern of membrane-bound mucins and side chain sugars in H. pylori associated-, NSAID-, and idiopathic-gastric ulcers.

Methods

We randomly selected 92 patients with H. pylori (group 1, n = 30), NSAID (group 2, n = 18), combined H. pylori and NSAID associated gastric ulcers (group 3, n = 24), and patients with idiopathic gastric ulcers (group 4, n = 20). Immunohistochemistry for T-cell CD4/CD8, MUC1, MUC4, MUC17, and ECA and SNA lectins staining was performed on sections from the ulcer margins. Inflammation score was assessed according to the Sydney system.

Results

Bleeding and mortality rates were significantly higher in group 4. CD4 positive T cell count was higher in H. pylori positive patients (P = 0.009). Staining intensity of MUC17 was higher in group 1 than in group 4, foveola and glands alike, with 11.50 ± 3.47 versus 6.80 ± 4.02, and 9.61 ± 4.26 versus 7.59 ± 3.26, respectively (P < 0.0001). This was a mirror image with MUC1. SNA lectin staining was increased in group 4, in parallel to MUC1 expression, indicating more abundant α2-6 sialylation in that group.

Conclusions

Cytoplasmic MUC17 staining was significantly decreased in the cases with idiopathic ulcer. The opposite was demonstrated for MUC1. This observation might be important, since different mucins with altered sialylation patterns likely differ in their protection efficiency against acid and pepsin.

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Acknowledgments

The work was supported by National Institute of Neurological Disorders and Stroke (grant P30 NS047101; Neurosciences Microscopy Shared Facility, UCSD) and grant *** CSD018 from the G. Harold and Leila Y. Mathers Charitable Foundation (P. Gagneux). Sukhwinder Kaur, Srustidhar Das, and Poonam Sharma performed the MUC4 staining.

Conflict of interest

None declared.

Author information

Authors and Affiliations

  1. Department of Gastroenterology, Rabin Medical Center, Tel Aviv University, 39 Jabotinski Street, 49100, Petach Tikva, Israel

    Yaron Niv, Doron Boltin, Zohar Levi & Alex Vilkin

  2. Department of Pathology, Rabin Medical Center, Hasharon Hospital, Petach Tikva, Israel

    Marisa Halpern

  3. Department of Cellular and Molecular Medicine, Glycobiology Research and Training Center, University of California at San Diego, San Diego, CA, USA

    Miriam Cohen & Pascal Gagneux

  4. Department of Pathology, Rabin Medical Center, Beilinson Hospital, Petach Tikva, Israel

    Sara Morgenstern

  5. Department of Medicine, University of California, San Diego, CA, USA

    Vahig Manugian, Erica St. Lawrence & Samuel B. Ho

  6. VA San Diego Healthcare System, San Diego, CA, USA

    Vahig Manugian, Erica St. Lawrence & Samuel B. Ho

  7. Department of Biochemistry and Molecular Biology, University of Nebraska, Omaha, NE, USA

    Surinder K. Batra

Authors
  1. Yaron Niv
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  2. Doron Boltin
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  5. Zohar Levi
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  9. Erica St. Lawrence
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  12. Samuel B. Ho
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Corresponding author

Correspondence to Yaron Niv.

Additional information

This article has been retracted due to plagiarism of content published in "Gastric mucin expression in Helicobacter pylori-related, nonsteroidal anti-inflammatory drug-related and idiopathic ulcers" by Doron Boltin, Marisa Halpern, Zohar Levi, Alex Vilkin, Sara Morgenstern, Samuel B Ho, Yaron Niv, published online in the World Journal of Gastroenterology on September 7, 2012, DOI: http://dx.doi.org/10.3748/wjg.v18.i33.4597.

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Niv, Y., Boltin, D., Halpern, M. et al. RETRACTED ARTICLE: Membrane-Bound Mucins and Mucin Terminal Glycans Expression in Idiopathic or Helicobacter pylori, NSAID Associated Peptic Ulcers. Dig Dis Sci 57, 2535–2544 (2012). https://doi.org/10.1007/s10620-012-2205-5

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  • DOI: https://doi.org/10.1007/s10620-012-2205-5

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