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Prevention of cardiotoxicity of aflatoxin B1 via dietary supplementation of papaya fruit extracts in rats

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Abstract

The aim of the current study was to evaluate the cardioprotective ability of water (WE) and ethanolic (EE) papaya fruits extracts against cardiotoxicity induced by aflatoxin B1 (AFB1) in rats. Forty two female Sprague–Dawley rats were divided into six treatment groups and treated orally for 2 weeks as follow: control group, the group treated with WE (250 mg/kg b.w), the group treated with EE (250 mg/kg b.w), the group treated orally with AFB1 (17 μg/kg b.w) and the groups treated orally with AFB1 plus WE or EE. The results indicated that treatment with AFB1 resulted in oxidative stress in the heart manifested by the marked increase in cardiac malondialdehyde and calcium levels accompanied with a significant decrease in cardiac total antioxidant capacity. Serum nitric oxide and sodium levels, lactate dehydrogenase and creatine kinase isoenzyme activities were significantly increased, whereas, cardiac Na+/K+-ATPase activity and serum potassium were insignificantly affected. Supplementation with WE or EE effectively ameliorated most of the changes induced by AFB1. It could be concluded that both extracts attenuated the oxidative stress induced in heart tissue by AFB1 and WE was more pronounced due to the higher total phenolic contents than in the EE.

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References

  • Abdel-Wahhab MA, Ahmed HH, Hagazi MM (2006) Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts. J Appl Toxicol 26:229–238

    Article  CAS  Google Scholar 

  • Abdel-Wahhab MA, Hassan NS, El-Kady AA, Khadrawy YA, El-Nekeety AA, Mohamed SR, Sharaf HA, Mannaa FA (2010) Red ginseng extract protects against aflatoxin B1 and fumonisins-induced hepatic pre-cancerous lesions in rats. Food Chem Toxicol 48:733–742

    Article  CAS  Google Scholar 

  • Abdulmajeed NA (2011) Therapeutic ability of some plant extracts on aflatoxin B1 induced renal and cardiac damage. Arab J Chem 4:1–10

    Article  CAS  Google Scholar 

  • Barnett RN, Skodon SB, Goldberg NH (1973) Performance of “kits” used for clinical chemical analysis of calcium in serum. Am J Clin Pathol 59:836–845

    CAS  Google Scholar 

  • Basu A, Haldar S (2008) Dietary isothiocyanate mediated apoptosis of human cancer cells is associated with Bcl-xL phosphorylation. Int J Oncol 33:657–663

    CAS  Google Scholar 

  • Berg D, Youdim MB, Riederer P (2004) Redox imbalance. Cell Tissue Res 318:201–213

    Article  Google Scholar 

  • Bernabucci U, Colavecchia L, Danieli PP, Basirico L, Lacetera N, Nardone A, Ronchi B (2011) Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheral blood mononuclear cells. Toxicol In Vitro 25:684–691

    Article  CAS  Google Scholar 

  • Calliste CA, Trouillas P, Allais DP, Simon A, Duroux JL (2001) Free radical scavenging activities measured by electron spin resonance spectroscopy and B16 cell antiproliferative behaviors of seven plants. J Agric Food Chem 49:3321–3327

    Article  CAS  Google Scholar 

  • Ching LS, Mohamed S (2001) Alpha-tocopherol content in 62 edible tropical plants. J Agric Food Chem 49:3101–3105

    Article  CAS  Google Scholar 

  • Choi KC, Chung WT, Kwon JK, Yu JY, Jang YS, Park SM, Lee SY, Lee JC (2010) Inhibitory effects of quercetin on aflatoxin B1-induced hepatic damage in mice. Food Chem Toxicol 48:2747–2753

    Article  CAS  Google Scholar 

  • Danson EJ, Choate JK, Paterson DJ (2005) Cardiac nitric oxide: emerging role of nNOS in regulating physiological function. Pharmacol Ther 106:57–74

    Article  CAS  Google Scholar 

  • Dixon MF, Nimmo J, Prescott LF (1971) Experimental paracetamol-induced hepatic necrosis: a histopathological study. J Pathol 103:225–229

    Article  CAS  Google Scholar 

  • El-Agamy DS (2010) Comparative effects of curcumin and resveratrol on aflatoxin B1-induced liver injury in rats. Arch Toxicol 84:389–396

    Article  CAS  Google Scholar 

  • Eno AE, Owo OI, Itam EH, Konya RS (2000) Blood pressure depression by the fruit juice of Carica papaya (L.) in renal and DOCA-induced hypertension in the rat. Phytother Res 14:235–239

    Article  CAS  Google Scholar 

  • Fresco P, Borges F, Marques MP, Diniz C (2010) The anticancer properties of dietary polyphenols and its relation with apoptosis. Curr Pharm Des 16:114–134

    Article  CAS  Google Scholar 

  • Galvano F, Piva A, Ritieni T, Galvano G (2001) Dietary strategies to counteract the effects of mycotoxins: a review. J Food Protect 64:120–131

    CAS  Google Scholar 

  • Guindon KA, Bedard LL, Massey TE (2007) Elevation of 8-hydroxydeoxyguanosine in DNA from isolated Mouse lung cells following in vivo treatment with aflatoxin B1. Toxicol Sci 98:57–62

    Article  CAS  Google Scholar 

  • Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochem 55:481–504

    Article  CAS  Google Scholar 

  • Hess DT, Matsumoto A, Kim SO, Marshall HE, Stamler JS (2005) Protein S-nitrosylation: purview and parameters. Nat Rev Mol Cell Biol 6:150–166

    Article  CAS  Google Scholar 

  • Huang KT, Han TH, Hyduke DR, Vaughn MW, Van Herle H, Hein TW, Zhang C, Kuo L, Liao JC (2001) Modulation of nitric oxide bioavailability by erythrocytes. Proc Natl Acad Sci USA 98:11771–11776

    Article  CAS  Google Scholar 

  • IARC (2002) Some traditional herbal medicines, some mycotoxins, naphthalene and styrene, IARC monographs on the evaluation of carcinogenic Risks to Humans. IARC 82:171–300

    Google Scholar 

  • IPCS-WHO (1998) Aflatoxins. In: WHO (ed) International programme on chemical safety, food additives, series 40. World Health Organization, Geneva, pp 1–73

  • Iyer D, Sharma BK, Patil UK (2011) Effect of ether- and water-soluble fractions of Carica papaya ethanol extract in experimentally induced hyperlipidemia in rats. Pharm Biol 49:1306–1310

    Article  CAS  Google Scholar 

  • Jayakumar R, Kanthimathi MS (2011) Inhibitory effects of fruit extracts on nitric oxide-induced proliferation in MCF-7 cells. Food Chem 126:956–960

    Article  CAS  Google Scholar 

  • Jeong JH, Jung H, Lee S, Lee H, Hwang KT, Kim T (2010) Anti-oxidant, anti-proliferative and anti-inflammatory activities of the extracts from black raspberry fruits and wine. Food Chem 123:338–344

    Article  CAS  Google Scholar 

  • Knowles RG, Moncada S (1994) Nitric oxide synthases in mammals. Biochem J 298:249–258

    CAS  Google Scholar 

  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V (2001) Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 54:356–361

    Article  CAS  Google Scholar 

  • Madamanchi NR, Vendrov A, Runge MS (2005) Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol 25:29–38

    Article  CAS  Google Scholar 

  • Mahattanatawee K, Manthey JA, Luzio G, Talcott S, Goodner KL, Baldwin EA (2006) Total antioxidant activity and fiber content of select Florida-grown tropical fruits. J Agric Food Chem 54:7355–7363

    Article  CAS  Google Scholar 

  • Masuda Y, Yano I, Sumida S, Murano T (1975) Studies on the function of cell membrane. 10th report: effects of CCl4 on the marker enzyme activities and fine structures of rat liver plasma membranes and microsomes in vitro. J Pharmacol 25:151–160

    CAS  Google Scholar 

  • Miean KH, Mohamed S (2001) Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. J Agric Food Chem 49:3106–3112

    Article  CAS  Google Scholar 

  • Negi PS, Jayaprakasha GK, Jena BS (2008) Antibacterial activity of the extracts from the fruit rinds of Garcinia cowa and Garcinia pedunculata against food borne pathogens and spoilage bacteria. LWT-Food Sci Technol 41:1857–1861

    Article  CAS  Google Scholar 

  • Osuna-Torres L, Tapia-Pérez ME, Aguilar-Contreras A (2005) Plantas medicinales de la medicina tradicional mexicana para tratar afecciones gastrointestinales: Estudio etnobotánico fitoquımico y farmacológico. Universidat de Barcelona, Barcelona

    Google Scholar 

  • Otsukia N, Dangb NH, Kumagaia E, Kondoc A, Iwataa S, Morimotoa C (2010) Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. J Ethnopharmacol 127:760–767

    Article  Google Scholar 

  • Park KH, Kim SY, Gul R, Kim BJ, Jang KY, Chung HT, Sohn DH (2008) Fatty acids ameliorate doxorubicin-induced intracellular Ca2+ increase and apoptosis in rat cardiomyocytes. Biol Pharma Bull 31:809–815

    Article  CAS  Google Scholar 

  • Pasha TN, Farooq MU, Khattak FM, Jabbar MA, Khan AD (2007) Effectiveness of sodium bentonite and two commercial products as aflatoxin absorbents in diets for broiler chickens. Anim Feed Sci Technol 132:103–110

    Article  CAS  Google Scholar 

  • Potluri S, Ventura HO, Mulumudi M, Mehra MR (2004) Cardiac troponin levels in heart failure. Cardiol Rev 12:21–25

    Article  Google Scholar 

  • Rajadurai M, Prince PS (2007) Preventive effect of naringin on isoproterenol-induced cardiotoxicity in Wistar rats: an in vivo and in vitro study. Toxicology 232:216–225

    Article  CAS  Google Scholar 

  • Rajkapoor B, Jayakar B, Kavimani S, Murugesh N (2002) Effect of dried fruits of Carica papaya linn on hepatotoxicity. Biol Pharm Bull 25:1645–1646

    Article  CAS  Google Scholar 

  • Rawal S, Kim JE, Coulombe R (2010) Aflatoxin B1 in poultry: toxicology, metabolism and prevention. Res Vet Sci 89:325–331

    Article  CAS  Google Scholar 

  • Ruiz-Larnea MB, Leal AM, Liza M, Lacort M, deGroot H (1994) Antioxidant effects of estradiol and 2-hydroxyestradiol on iron induced lipid peroxidation of rat liver microsome. Steroid 59:383–388

    Article  Google Scholar 

  • Runnie I, Salleh MN, Mohamed S, Head RJ, Abeywardena MY (2004) Vasorelaxation induced by common edible tropical plant extracts in isolated rat aorta and mesenteric vascular. J Ethnopharmacol 92:311–316

    Article  CAS  Google Scholar 

  • Rustom IYS (1997) Aflatoxin in food and feed: occurrence, legislation and inactivation by physical methods. Food Chem 59:57–67

    Article  CAS  Google Scholar 

  • Schinella GR, Tournier HA, Prieto JM, Mordujovich de Buschiazzo P, Rios JL (2002) Antioxidant activity of anti-inflammatory plant extracts. Life Sci 70:1023–1033

    Article  CAS  Google Scholar 

  • Scott MG, Heusel JW, LeGrys VA, Siggard-Anderson O (1999) Renal electrolytes and blood gases. In: Burtis CA, Ashwood ER (eds) Tietz textbook of clinical chemistry. W.B. Sawnders Company, Philadelphia, pp 1056–1092

    Google Scholar 

  • Seigler DS, Pauli GF, Nahrstedt A, Leen R (2002) Cyanogenic allosides and glucosides from Passiflora edulis and Carica papaya. Phytochem 60:873–882

    Article  CAS  Google Scholar 

  • Singal PK, Kirshenbaum LA (1990) A relative deficit in antioxidant reserve may contribute in cardiac failure. Can J Cardiol 6:47–49

    CAS  Google Scholar 

  • Stangeland T, Siv F, Remberg SF, Kare A, Lye KA (2009) Total antioxidant activity in 35 Ugandan fruits and vegetables. Food Chem 113:85–91

    Article  CAS  Google Scholar 

  • Steel RG, Torrie GH (1960) Principles and procedures of statistics and biometrical approach, 2nd edn. McGraw-Hill Book Co., New York, pp 71–117

    Google Scholar 

  • Tamer L, Ercan B, Unlu A, Sucu N, Pekdemir H, Eskandari G, Atik U (2002) The relationship between leptin and lipids in atherosclerosis. Indian Heart J 54:692–696

    CAS  Google Scholar 

  • Tian J, Cai T, Yuan Z, Wang H, Liu L, Haas M, Maksimova E, Huang XY, Xie ZJ (2006) Binding of Src to Na+/K+-ATPase forms a functional signaling complex. Mol Biol Cell 17:317–326

    Article  CAS  Google Scholar 

  • Tsakiris S, Angelogianni P, Schulpis KH, Behrakis P (2000) Protective effect of l-cysteine and glutathione on rat brain Na+/K+-ATPase inhibition induced by free radical. Z Naturforsch C 55:271–277

    CAS  Google Scholar 

  • Tsakiris S, Schulpis KH, Marinou K, Behrakis P (2004) Protective effect of l-cysteine and glutathione on the modulated sukling rat brain Na+/K+-ATPase and Mg2+-ATPase activities induced by the in vitro gulactosaemia. Pharmacol Res 49:475–479

    Article  CAS  Google Scholar 

  • Ungvari Z, Gupte SA, Recchia FA, Batkai S, Pacher P (2005) Role of oxidative-nitrosative stress and downstream pathways in various forms of cardiomyopathy and heart failure. Curr Vasc Pharmacol 3:221–229

    Article  CAS  Google Scholar 

  • Van Breemen RB, Pajkovic N (2008) Multitargeted therapy of cancer by lycopene. Cancer Lett 269:339–351

    Article  Google Scholar 

  • Wangikar PB, Dwivedi P, Sinha N, Sharma AK, Telang AG (2005) Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicology 215:237–247

    Article  Google Scholar 

  • WHO (1998) Forty-ninth report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series. WHO, Geneva

    Google Scholar 

  • Wild CP, Gong YY (2010) Mycotoxins and human disease: a largely ignored global health issue. Carcinogenesis 31:71–82

    Article  CAS  Google Scholar 

  • Yee KC, Mukherjee D, Smith DE, Kline-Rogers EM, Fang J, Mehta RH, Almanaseer Y, Akhras E, Cooper JV, Eagle KA (2003) Prognostic significance of an elevated creatine kinase in the absence of an elevated troponin I during an acute coronary syndrome. Am J Cardiol 92:1442–1444

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by National Research Center, Dokki, Cairo, Egypt project # S90402.

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The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Medical Physiology Department, National Research Centre, Dokki, Cairo, Egypt

    Fathia A. Mannaa & Khaled G. Abdel-Wahhab

  2. Food Toxicology and Contaminants Department, National Research Centre, Dokki, Cairo, Egypt

    Mosaad A. Abdel-Wahhab

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  1. Fathia A. Mannaa
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  2. Khaled G. Abdel-Wahhab
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Correspondence to Mosaad A. Abdel-Wahhab.

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Mannaa, F.A., Abdel-Wahhab, K.G. & Abdel-Wahhab, M.A. Prevention of cardiotoxicity of aflatoxin B1 via dietary supplementation of papaya fruit extracts in rats. Cytotechnology 66, 327–334 (2014). https://doi.org/10.1007/s10616-013-9579-x

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