Abstract
A common dietary contaminant, aflatoxin B1 (AFB1), has been shown to be a potent mutagen and carcinogen in humans and many animal species. Since the eradication of AFB1 contamination in agricultural products has been rare, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boron compounds like borax (BX) and boric acid are the major components of industry and their antioxidant role has recently been reported. In the present report, we evaluated the capability of BX to inhibit the rate of micronucleus (MN) and sister chromatid exchange (SCE) formations induced by AFB1. There were significant increases (P < 0.05) in both SCE and MN frequencies of cultures treated with AFB1 (3.12 ppm) as compared to controls. However, co-application of BX (1, 2 and 5 ppm) and AFB1 resulted in decreases of SCE and MN rates as compared to the group treated with AFB1 alone. Borax gave 30–50 % protection against AFB1 induced SCEs and MNs. In conclusion, the support of borax was especially useful in aflatoxin-toxicated blood tissue. Thus, the risk on target tissues of AFB1 could be reduced and ensured early recovery from its toxicity.
References
Adam G, Malgorzata KL (2008) Protective effects of melatonin and N-acetylserotonin on aflatoxin B1-induced lipid peroxidation in rats. Cell Biochem Funct 26:314–319
Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DE, Tice R, Waters MD, Aitio A (2000) IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat Res 463:111–172
Amici M, Cecarini V, Pettinari A, Bonfili L, Angeletti M, Barocci S, Biagetti M, Fioretti E, Maria EA (2007) Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis. Biol Chem 388:107–117
Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516
Bozkurt G, Yuksel M, Karabogaz G, Sut N, Savran FO, Palanduz S, Yigitbasi ON, Algunes C (2003) Sister chromatid exchanges in lymphocytes of nuclear medicine physicians. Mutat Res 535:205–213
Devirian TA, Volpe SL (2003) The physiological effects of dietary boron. Crit Rev Food Sci Nutr 43:219–231
Evans HJ, O’Riordan ML (1975) Human lymphocytes for the analysis of chromosome aberrations in mutagen tests. Mutat Res 31:135–148
Fenech M (1993) The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat Res 285:35–44
Ferguson LR, Philpott M, Karunasinghe N (2004) Dietary cancer and prevention using antimutagens. Toxicology 198:147–159
Fraga CG, Tappel AL (1988) Damage to DNA concurrent with lipid peroxidation in rat liver slices. Biochem J 252:893–896
Geyikoglu F, Turkez H (2005) Protective effect sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1. Braz Arch Biol Technol 48:905–910
Geyikoglu F, Turkez H (2008) Boron compounds reduce vanadium tetraoxide genotoxicity in human lymphocytes. Environ Toxicol Pharmacol 26:342–347
Groopman JD, Kensler TW (1999) The light at the end of the tunnel for chemical- specific biomarkers: daylight or headlight? Carcinogenesis 20:1–11
Guindon KA, Bedard LL, Massey TE (2007) Elevation of 8-hydroxydeoxyguanosine in DNA from isolated mouse lung cells following in vivo treatment with aflatoxin b1. Toxicol Sci 98:57–62
Hedenborg M (1988) Titanium dioxide induced chemiluminescence of human polymorphonuclear leukocytes. Int Arch Occup Environ Health 61:1–6
Hooiveld M, Heederick DJJ, Kogevinas M, Boffetta P, Needham LL, Patterson DG, Bueno-de-Mesquita HB Jr (1998) Second follow-up of a Dutch cohort occupationally exposed to phenoxy herbicides, chlorophenols, and contaminants. Am J Epidemiol 147:891–901
Hunt CD, Idso JP (1999) Dietary boron as a physiological regulator of the normal inflammatory response: a review and current research progress. J Trace Elem Exp Med 12:221–233
Leopold F (1976) Glutathione peroxidase brought into focus. In: Pryor WA (ed) Free radicals in biology. Academic Press, New York, pp 223–254
McConkey DJ, Hartzell P, Jondal M, Orrenius S (1989) Inhibition of DNA fragmentation in thymocytes and isolated thymocyte nuclei by agents that stimulate protein kinase C. J Biol Chem 264:13399–13402
Meister A (1983) Selective modification of glutathione metabolism. Science 220:472–477
Pawa S, Ali S (2006) Boron ameliorates fulminant hepatic failure by counteracting the changes associated with the oxidative stress. Chem Biol Interact 160:89–98
Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158
Rahman Q, Lohani M, Dopp E, Pemsel H, Jonas L, Weiss DG, Schiffmann D (2002) Evidence that titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environ Health Perspect 110:797–800
Rastogi R, Srivastava AK, Rastogi AK (2001) Long term effect of aflatoxin B(1) on lipid peroxidation in rat liver and kidney: effect of picroliv and silymarin. Phytother Res 15:307–310
Shen HM, Shi CY, Shen Y, Ong CN (1996) Detection of elevated reactive oxygen species level in cultured rat hepatocytes treated with aflatoxin B1. Free Radic Biol Med 21:139–146
Shon MY, Choi SD, Kahng GG (2004) Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem Toxicol 42:659–666
Turkez H (2008) Effects of boric acid and borax on titanium dioxide genotoxicity. J Appl Toxicol 28:658–664
Turkez H, Geyikoglu F (2010) Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood. Cytotechnology 62:157–165
Turkez H, Sisman T (2007) Anti-genotoxic effect of hydrated sodium calcium aluminosilicate on genotoxicity to human lymphocytes induced by aflatoxin B-1. Toxicol Ind Health 23:83–89
Turkez H, Geyikoglu F, Tatar A, Keleş S, Özkan A (2007) Effects of some boron compounds on peripheral human blood. Z Naturforsch C 62:889–896
Turkez H, Tatar A, Hacimuftuoglu A, Ozdemir E (2010a) Boric acid as a protector against paclitaxel genotoxicity. Acta Biochim Pol 57:95–97
Turkez H, Geyikoglu F, Aslan A, Karagöz Y, Türkez O, Anar M (2010b) Antimutagenic effects of lichen Pseudovernia furfuracea (L.) Zoph. extracts against the mutagenicity of aflatoxin B(1) in vitro. Toxicol Ind Health 26:625–631
Turkez H, Geyikoglu F, Colak S (2011) The protective effect of boric acid on aluminum-induced hepatotoxicity and genotoxicity in rats. Turk J Biol 35:293–301
Turkez H, Geyikoglu F, Tatar A, Keles MS, Kaplan İ (2012) The effects of some boron compounds against heavy metal toxicity in human blood. Exp Toxicol Pathol 64:93–101
Umarani M, Shanthi P, Sachdanandam P (2008) Protective effect of Kalpaamruthaa in combating the oxidative stress posed by aflatoxin B1-induced hepatocellular carcinoma with special reference to flavonoid structure–activity relationship. Liver Int 28:200–213
Weitberg AB, Weitzman SA, Destrempes M, Latt SA, Stossel TP (1983) Stimulated human phagocytes produce cytogenetic changes in cultured mammalian cells. N Engl J Med 308:26–30
Xi ZG, Chao FH, Yang DF, Sun YM, Li GX, Zhang HS, Zhang W, Yang YH, Liu HL (2004) The effects of DNA damage induced by acetaldehyde. Huan Jing Ke Xue 25:102–105
Yamashoji S, Isshiki K (2001) Rapid detection of cytotoxicity of food additives and contaminants by a novel cytotoxicity test, menadione-catalyzed H2O2 production assay. Cytotechnology 37:171–178
Acknowledgments
We are grateful to volunteers for the blood samples. The author is grateful to Dr. Savaş Yeşilyurt for his proofreading the article.
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Turkez, H., Geyikoğlu, F., Dirican, E. et al. In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes. Cytotechnology 64, 607–612 (2012). https://doi.org/10.1007/s10616-012-9454-1
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DOI: https://doi.org/10.1007/s10616-012-9454-1