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New polymorphic microsatellite loci for Theobroma cacao: isolation and characterization of microsatellites from enriched genomic libraries

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Biologia Plantarum

Abstract

Seventeen polymorphic microsatellite markers were isolated from enriched genomic libraries for Theobroma cacao, providing additional tools for studying the genetic diversity and map saturation of this species. These markers were characterized in 32 accessions of the T. cacao germplasm collection from the Centro de Pesquisas do Cacau. The number of alleles at each locus varied from 2 to 8, with an average of 4.41 alleles per locus. The polymorphism information content varied from 0.060 to 0.695, with an average of 0.333. The markers characterized in this study will be employed in map saturation studies and diversity assessments of cacao genotypes.

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Abbreviations

CTAB:

cetyltrimethylammonium bromide

HWE:

Hardy-Weinberg equilibrium

LD:

linkage disequilibrium

PCR:

polymerase chain reaction

PIC:

polymorphism information content

SSR:

simple sequence repeat

TBE:

Tris/borate/EDTA

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Authors and Affiliations

  1. Departamento de Estudos Básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Rodovia BR415, Km03, s/n, Itapetinga, 45700-000, Bahia, Brazil

    E. S. L. Santos & C. B. M. Cerqueira-Silva

  2. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Av. Cândido Rondon, 400, CP 6010, Campinas, 13083-875, São Paulo, Brazil

    E. S. L. Santos, C. B. M. Cerqueira-Silva, G. M. Mori & A. P. Souza

  3. Centro de Biotecnologia e Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna Km 16, Ilhéus, 45662-900, Bahia, Brazil

    D. Ahnert & R. X. Corrêa

  4. Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, Campinas, 13083-970, São Paulo, Brazil

    A. P. Souza

Authors
  1. E. S. L. Santos
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  2. C. B. M. Cerqueira-Silva
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  3. G. M. Mori
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  4. D. Ahnert
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  5. R. X. Corrêa
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  6. A. P. Souza
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Corresponding author

Correspondence to A. P. Souza.

Additional information

Acknowledgments: The authors are grateful to Conselho de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, PROCADNF2008) for financial support, to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing graduate fellowships to G.M. Mori and E.S.L. Santos, respectively, and a research fellowship (from CNPq) to A.P. Souza.

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Santos, E.S.L., Cerqueira-Silva, C.B.M., Mori, G.M. et al. New polymorphic microsatellite loci for Theobroma cacao: isolation and characterization of microsatellites from enriched genomic libraries. Biol Plant 56, 789–792 (2012). https://doi.org/10.1007/s10535-012-0134-y

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  • DOI: https://doi.org/10.1007/s10535-012-0134-y

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