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Fermentative capabilities and volatile compounds produced by Kloeckera/Hanseniaspora and Saccharomyces yeast strains in pure and mixed cultures during Agave tequilana juice fermentation

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Abstract

The fermentative and aromatic capabilities of Kloeckera africana/Hanseniaspora vineae K1, K. apiculata/H. uvarum K2, and Saccharomyces cerevisiae S1 and S2 were studied in pure and mixed culture fermentations using Agave tequila juice as the culture medium. In pure and mixed cultures, Kloeckera/Hanseniaspora strains showed limited growth and sugar consumption, as well as low ethanol yield and productivity, compared to S. cerevisiae, which yielded more biomass, ethanol and viable cell concentrations. In pure and mixed cultures, S. cerevisiae presented a similar behaviour reaching high biomass production, completely consuming the sugar, leading to high ethanol production. Furthermore, the presence of S. cerevisiae strains in the mixed cultures promoted the production of higher alcohols, acetaldehyde and ethyl esters, whereas Kloeckera/Hanseniaspora strains stimulated the production of ethyl acetate and 2-phenyl ethyl acetate compounds.

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Acknowledgments

This research was funded by CONACYT (Mexican National Council of Science and Technology) under the project SEP-CONACYT 24547. Ivonne Wendolyne Gonzalez Robles thanks CONACYT for the scholarships granted. The authors would also like to thank John Dye of Peace Corps Mexico for revising the language of the manuscript.

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Author notes

  1. Ivonne Wendolyne González-Robles

    Present address: Brewing Science Section, Division of Food Sciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, UK

Authors and Affiliations

  1. Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico

    Ivonne Wendolyne González-Robles & Dulce María Díaz-Montaño

  2. Unidad de Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico

    Mirna Estarrón-Espinosa

Authors
  1. Ivonne Wendolyne González-Robles
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  2. Mirna Estarrón-Espinosa
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  3. Dulce María Díaz-Montaño
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Corresponding author

Correspondence to Ivonne Wendolyne González-Robles.

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Dulce María Díaz-Montaño—Research leader retired.

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González-Robles, I.W., Estarrón-Espinosa, M. & Díaz-Montaño, D.M. Fermentative capabilities and volatile compounds produced by Kloeckera/Hanseniaspora and Saccharomyces yeast strains in pure and mixed cultures during Agave tequilana juice fermentation. Antonie van Leeuwenhoek 108, 525–536 (2015). https://doi.org/10.1007/s10482-015-0506-3

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