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Negadavirga shengliensis gen. nov., sp. nov., a novel member of the family Cyclobacteriaceae isolated from oil-contaminated saline soil

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An Erratum to this article was published on 21 March 2015

Abstract

Four novel Gram-stain-negative, rod-shaped, and non-motile bacterial strains, SLG210-21T, SLG210-4, SLG210-5 and SLG210-14, were isolated from oil-contaminated saline soil in Shengli Oilfield, China. Growth were observed at 25–42 °C (optimum 37 °C), in the presence of 0–10 % (w/v) NaCl (optimum 0–1 %) and at pH 4.0–10.0 (optimum pH 7.6–8.6). All the strains were positive for catalase and α, β-galactosidase activities and nitrogen reduction, and negative for oxidase activity, glucose fermentation and hydrolysis of agar, starch, gelatin, Tween 40, 60 and 80. The DNA G+C contents of the four strains were 41.3–43.0 mol% and the predominant respiratory quinones were all menaquinone-7. The major fatty acids were iso-C15:0, anteiso-C15:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:1 ω5c and summed feature 9 (iso-C17:1 ω9c and/or 10-methyl C16:0), while the polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, glycolipid, two unidentified phospholipids and two unidentified amino lipids. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains clustered together to form a stable branch in the family Cyclobacteriaceae, and were most closely related to the genera Cyclobacterium and Echinicola with the 16S rRNA gene sequence similarities being 88.6–90.3 and 89.6–91.4 %, respectively. DNA–DNA hybridization between SLG210-21T and the other three strains showed the relatedness of 93.8 ± 4.5, 96.2 ± 4.2 and 82.3 ± 4.8 %, respectively. Based on the polyphasic analysis, a novel species in a new genus, Negadavirga shengliensis gen. nov., sp. nov., is proposed with SLG210-21T (=LMG 27734T = CGMCC1.12768T) as the type strain.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31225001) and National High Technology Research and Development Program of China (2012AA02A703).

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Authors and Affiliations

  1. College of Engineering, Peking University, Beijing, 100871, People’s Republic of China

    Bing Hu, Qian Yang, Gui-Fang Zhao & Xiao-Lei Wu

  2. Institute of Microbiology Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Man Cai

  3. College of Architecture and Environment, Sichuan University, Chengdu, 610065, People’s Republic of China

    Yue-Qin Tang

  4. Institute of Engineering (Baotou), College of Engineering, Peking University, Baotou, 014030, People’s Republic of China

    Xiao-Lei Wu

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  1. Bing Hu
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  2. Qian Yang
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  5. Gui-Fang Zhao
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Corresponding author

Correspondence to Xiao-Lei Wu.

Additional information

Bing Hu and Qian Yang have contributed equally to this work.

Sequence deposited: The GenBank accession numbers for the 16S rRNA gene sequences of strains SLG210-21T, SLG210-4, SLG210-5 and SLG210-14 are KF309029, KF309030, KF309028 and KF309027, respectively.

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Hu, B., Yang, Q., Cai, M. et al. Negadavirga shengliensis gen. nov., sp. nov., a novel member of the family Cyclobacteriaceae isolated from oil-contaminated saline soil. Antonie van Leeuwenhoek 107, 663–673 (2015). https://doi.org/10.1007/s10482-014-0361-7

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