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Isoptericola rhizophila sp. nov., a novel actinobacterium isolated from rhizosphere soil

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Abstract

A Gram-positive, yellow pigmented strain, BKS 3-46T was isolated from a soil sample collected from the rhizosphere of Ficus benghalensis (banyan tree) in Bhitarkanika mangrove forest, in the Indian state of Odisha, and subjected to polyphasic taxonomic study. The 16S rRNA gene sequence of the strain was determined and the sequence analysis showed that strain BKS 3-46T should be assigned to the genus Isoptericola. The chemotaxonomic data supported this taxonomic placement i.e. presence of menaquinone MK-9(H4); major fatty acids anteiso C15:0 and iso-C15:0; and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol (PI) as major polar lipids. Further phylogenetic analysis of the 16S rRNA gene sequence confirmed that the strain BKS 3-46T belongs to the genus Isoptericola and is closely related to Isoptericola halotolerans MTCC 11265T (98.6 %) followed by Isoptericola nanjingensis MTCC 11633T (98.4 %) and Isoptericola chiayiensis MTCC 11634T (98.1 %). However, the DNA–DNA hybridization values obtained between strain BKS 3-46T and other related strains were well below the threshold that is required for the proposal of a novel species. The G+C content of the genomic DNA was determined to be 70.4 mol%. The phenotypic and genotypic data showed that the strain BKS 3-46T merits the recognition as a representative of a novel species of the genus Isoptericola. It is proposed that the isolate should be classified in the genus Isoptericola as a novel species, Isoptericola rhizophila sp. nov. The type strain is BKS 3-46T (=MTCC 11080T=JCM 19252T).

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Acknowledgments

We thank Mr. Malkit Singh for his excellent technical assistance. We also thank Mr. Nitin Kumar Singh, Ms. Ishwinder Kaur and Ms. Monu Bala for their assistance in collection and preliminary work. This work was supported by Council of Scientific and Industrial Research and Department of Biotechnology, Government of India. This is IMTECH communication number 58/2013.

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Authors and Affiliations

  1. Microbial Type Culture Collection & Gene Bank (MTCC), CSIR - Institute of Microbial Technology (IMTECH), Sector 39-A, Chandigarh, 160 036, India

    Navjot Kaur, Mathan Kumar Rajendran, Gurwinder Kaur & Mayilraj Shanmugam

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  1. Navjot Kaur
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  2. Mathan Kumar Rajendran
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  3. Gurwinder Kaur
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  4. Mayilraj Shanmugam
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Correspondence to Mayilraj Shanmugam.

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Kaur, N., Rajendran, M.K., Kaur, G. et al. Isoptericola rhizophila sp. nov., a novel actinobacterium isolated from rhizosphere soil. Antonie van Leeuwenhoek 106, 301–307 (2014). https://doi.org/10.1007/s10482-014-0197-1

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  • DOI: https://doi.org/10.1007/s10482-014-0197-1

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