Abstract
The effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l−1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l−1, at D=0.05–0.3 h−1, and from 60–70 g l−1, at D=0.05 and 0.1 h−1·30 g l−1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l−1 h−1, was achieved at D=0.30 h−1 and 60 g l−1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l−1 of feed glycerol and a dilution rate higher than 0.10 h−1; moreover, at D=0.30 h−1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.
Similar content being viewed by others
References
Abbad-Andaloussi S, Dürr C, Raval G, Petitdemange H (1996) Carbon and electron flow in Clostridium butyricum grown in chemostat culture on glycerol and glucose. Microbiology 142:1149–1158
Biebl H, Marten S, Hippe H, Deckwer W-D (1992) Glycerol conversion to 1,3-propanediol by newly isolated clostridia. Appl Microbiol Biotechnol 36:592–597
Biebl H, Menzel K, Zeng A-P, Deckwer W-D (1999) Microbial production of 1,3-propanediol. Appl Microbiol Biotechnol 52:289–297
González-Pajuelo M, Andrade JC, Vasconcelos I (2004) Production of 1,3-propanediol by Clostridium butyricum VPI 3266 using a synthetic medium and raw glycerol. J Ind Microbiol Biotechnol 31:442–446
Hall RH, Stern ES (1950) Acid-Catalysed hydration of acraldehyde. Kinetics of the reaction and isolation of β- Hydroxypropaldehyde. J Chem Soc 1950:490–498
Hartlep M, Hussmann W, Prayitno N, Meynial-Salles I, Zeng AP (2002) Study of two-stage processes for the microbial production of 1,3-propanediol from glucose. Appl Microbiol Biotechnol 60:60–66
Heyndrickx M, de Vos P, Vancanneyt M, de Ley J (1991) The fermentation of glycerol by Clostridium butyricum LMG 1212t2 and 1213t1 and Clostridium pasteurianum LMG 3285. Appl Microbiol Biotechnol 34:637–642
Homann T, Tag C, Biebl H, Deckwer W-D, Schink B (1990) Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citobacter strains. Appl Microbiol Biotechnol 33:121–126
Menzel K, Zeng A-P, Deckwer W-D (1997) High concentration and productivity of 1,3-propanediol from continuous fermentation of glycerol by Klebsiella pneumoniae. Enzyme Microbiol Technol 28:82–86
Michel-Savin D, Marchal R, Vandecasteele JP (1990) Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition. Appl Microbiol Biotechnol 33:127–131
Pflugmacher U, Gottschalk G (1994) Development of an immobilized cell reactor for the production of 1,3-propanediol by Citrobacter freundii. Appl Microbiol Biotechnol 41:313–316
Reimann A, Biebl H, Deckwer W-D (1998) Production of 1,3-propanediol by Clostridium butyricum in continuous culture with cell recycle. Appl Microbiol Biotechnol 49:359–363
Saint-Amans S, Girbal L, Andrade J, Ahrens K, Soucaille P (2001) Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures. J Bacteriol 183:1748–1754
Saint-Amans S, Perlot P, Goma G, Soucaille P (1994) High production of 1,3-propanediol from glycerol by Clostridium butyricum in a simply controlled fed-batch system. Biotechnol Lett 17:211–216
Saint-Amans S, Soucaille P (1995) Carbon and electron flow in Clostridium butyricum VPI 3266 grown in chemostat culture on glucose-glycerol mixtures. Biotecnol Lett 17:211–216
Solobov M, Smiley KL (1960) Metabolism of glycerol by an acrolein-forming lactobacillus. J Bacteriol 79:261–266
Vasconcelos I, Girbal L, Soucaille P (1994) Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol. J Bacteriol 176:1443–1450
Zeng A-P (1996) Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum. Bioprocess Eng 14:169–175
Acknowledgements
This work was financially supported by the European FAIR project (Contract No. FAIR-CT96-1912). M.González-Pajuelo was supported by PRAXIS XXI with a PhD grant (BD/16036/98).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
González-Pajuelo, M., Andrade, J.C. & Vasconcelos, I. Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity. J IND MICROBIOL BIOTECHNOL 32, 391–396 (2005). https://doi.org/10.1007/s10295-005-0012-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10295-005-0012-0